Die Kulturpflanze

, Volume 30, Issue 2, pp 155–165 | Cite as

Untersuchungen zur Kopplung zwischen induzierten Translokationen und Genen für männliche Sterilität bei Gerste

  • Gottfried Künzel
  • Friedrich Scholz
Originalarbeiten

Zusammenfassung

Untersucht werden die Ergebnisse von F3-Kopplungstests an insgesamt 111 Kombinationen zwischen 47 verschiedenen Translokationen und 5 Genen für männliche Sterilität:msg1 undmsg4 (Chromosom 5),msg6 (Chromosom 6),msg24 undmsg25 (Chromosom 4).

Die 5msg-Gene zeigen entweder mit allen, mit nur einem Teil oder mit keiner der geprüften Translokationen enge Kopplung. Ihre Eignung für Systeme der Hybridsaatguterzeugung ist daher sehr unterschiedlich. Die Ergebnisse erlauben den Schluß, daßmsg24, msg6 und auchmsg1 in der zentromernahen Region ihrer Chromosomen liegen. Dagegen habenmsg4 undmsg25 sicherlich zentromerferne Positionen.

Durch cytologische Analysen von BTT-Formen konnte für 3 Gene bestimmt werden, auf welchem Chromosomenarm sie lokalisiert sind:msg4 auf dem langen Arm,msg6 undmsg24 auf den kurzen Armen. Darüber hinaus wird in zwei Fällen (msg6 undmsg24) eine markante Differenz zwischen rekombinativer und cytologischer Distanz zum Zentromer nachgewiesen.

Linkage studies with induced translocations and genes for male sterility in barley

Summary

Results of F3 linkage tests were analysed for 111 combinations between 47 different translocations and 5 genes for male sterility:msg1 andmsg4 (chromosome 5),msg6 (chromosome 6),msg24 andmsg25 (chromosome 4).

The 5 genes show close linkage with all, or with a part only, or even with not any of the translocations tested. Therefore, they are differently useful for systems of hybrid seed production. The results indicate thatmsg24, msg6, and alsomsg1 are located in or close to the centromere regions of their chromosomes. On the other hand,msg4 andmsg25 are certainly in distant positions from the centromere regions.

Cytological analyses of BTT stocks ascertained the respective chromosome arms for 3 genes:msg4 on the long arm,msg6 andmsg24 on the short arms. Moreover, marked differences can be demonstrated between genetic and cytological centromere distances in two cases (msg6 andmsg24); this is obviously caused by high heterochromatin content of the chromosome arms involved.

Краткое содержание Исследования о сцепл ении между индуциров анными транслокацпями и ген ами

мужской стерильност и у ячменя

Сообщаетсяорезульт атах анализа сцеплен ия в F3 у 111 комбинаций между 47 раз личными транслокациями и пят ью генами мужской сте рильности:msg 1 иmsg4 (хромосома 5),msg6 (хромосо ма 6),msg24 и msg25 (хромосома 4). Эти пятьmsg-генов обнар ужили тесное сцеплен ие или со всеми, или только с частью, или ни с одной т ранслокацией. Для сис тем получения гибридног о посевного материала ячменя их п ригодность, таким обр азом, оказывается весьма р азличной. Наши результаты позв оляют сделать заключ ение о том, чтоmsg24, msg6 иmsg1 расположены в облас ти центромеров их хро мосом, в то время как геныmsg4 иmsg25 должны быть локали зированы в отдалении от центромеров. При помощи цитологич еского анализа форм В ТТ, для трех генов удалось впервы е установить их локали зацию на определенны х плечах хромосомы:msg4 — на длинном плече, amsg6 иmsg24 — н а коротких. Кроме того, в двух случаях (msg6 иmsg24) можно было показать существенную разниц у между рекомбинативной и цитологической дист анциями генов от цент ромера.

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Copyright information

© Akademie-Verlag 1982

Authors and Affiliations

  • Gottfried Künzel
    • 1
  • Friedrich Scholz
    • 1
  1. 1.Zentralinstitut für Genetik und Kulturpflanzenforschung der Akademie der Wissenschaften der DDRGaterslebenDDR

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