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Use of the enzyme-linked immunosorbent assay for detection of antibodies toChlamydia trachomatis

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Abstract

An enzyme-linked immunosorbent assay (ELISA) using a soluble antigen prepared from the D strain ofChlamydia trachomatis was used for titration of IgG antibodies toChlamydia trachomatis in 153 sera from 126 patients with non-specific genital infection and from 27 healthy subjects. The results were compared to those obtained with the micro-immunofluorescence method and were in complete agreement in 143 of the 153 sera. The ELISA proved to be reproducible and as sensitive as the micro-immunofluorescence method.

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References

  1. Schachter, J.: Chlamydial infections. New England Journal of Medicine 1978, 298: 428–435.

    PubMed  Google Scholar 

  2. Grayston, J. L., Wang, S. P.: New knowledge of chlamydiae and the diseases they cause. Journal of Infectious Diseases 1975, 132: 87–105.

    PubMed  Google Scholar 

  3. Philip, R. N., Lackman, D. B., Franck, F. W., Morrisson, J. D., Casper, E. A., Greaves, A. B.: Serologic reactions to specific complement fixing antigens from microorganisms of the psittacosislymphogranuloma venereum trachoma group. American Journal of Ophtalmology 1967, 63: 1499–1502.

    Google Scholar 

  4. Wang, S. P., Grayston, J. T.: Immunologic relationship between genital TRIC, lymphogranuloma venereum, and related organisms in a new microtiter indirect immunofluorescence test. American Journal of Ophtalmology 1970, 70: 367–374.

    Google Scholar 

  5. Wang, S. P., Grayston, J. T.: Human serology inChlamydia trachomatis infection with microimmunofluorescence. Journal of Infectious Diseases 1974, 130: 388–397.

    PubMed  Google Scholar 

  6. Treharne, J. D., Darougar, S., Jones, B. R.: Modification of the microimmunofluorescence test to provide a routine serodiagnostic test for chlamydial infection. Journal of Clinical Pathology 1977, 30: 510–517.

    PubMed  Google Scholar 

  7. Thomas, B. J., Reeve, P., Oriel, J. D.: Simplified serological test for antibodies toChlamydia trachomatis. Journal of Clinical Microbiology 1976, 4: 6–10.

    PubMed  Google Scholar 

  8. Thomas, D., Orfila, J., Bissac, E., Corbel, C., Herve, M.: Comparaison de deux techniques d'immunofluorescence pour la détection d'anticorps anti-chlamydiens. Journal of Biological Standardization 1982, 10: 311–318.

    PubMed  Google Scholar 

  9. Engvall, E., Perlmann, P.: Enzyme-linked immunosorbent assay. Quantitative assay of immunoglobulin G. Immunochemistry 1971, 8: 871–874.

    Article  PubMed  Google Scholar 

  10. Van Weemen, B. K., Schuurs, A. H. W. M.: Immunoassay using antigen-enzyme conjugate. FEBS Letters 1971, 15: 232–237.

    Article  PubMed  Google Scholar 

  11. Avrameas, S., Guilbert, B.: Enzyme immunoassay for the measurement of antigen conjugates. Biochimie 1972, 54: 837–842.

    PubMed  Google Scholar 

  12. Lewis, V. J., Thacker, W. L., Mitchell, S. H.: Enzyme-linked immunosorbent assay for chlamydial antibodies. Journal of Clinical Microbiology 1977, 6: 507–510.

    PubMed  Google Scholar 

  13. Goldstein, F. W., Duc-Goiran, P., Acar, J. F.: Les uretrites à germes banals. Gazette Medicale de France 1979, 39: 4563–4570.

    Google Scholar 

  14. Jones, B. R., Collier, L. H., Smith, C. H.: Isolation of virus from inclusion blennorrhoea. Lancet 1959, i: 902–905.

    Article  Google Scholar 

  15. Wang, S. P., Grayston, J. T.: Classification of TRIC and related strains with microimmunofluorescence. In: Nichols, R. L. (ed.): Trachoma and related disorders caused by chlamydial agents. Excerpta Medica, Amsterdam, 1971, p. 305–321.

    Google Scholar 

  16. Howard, L., Orenstein, N. S., King, N. W.: Purification on renografin density gradients ofChlamydia trachomatis grown in the yolk sac of eggs. Applied Microbiology 1974, 27: 102–106.

    PubMed  Google Scholar 

  17. Furness, G., Granham, D. M., Reeve, P.: The titration of trachoma and inclusion blennorrhoea viruses in cell cultures. Journal of General Microbiology 1960, 23: 613–619.

    PubMed  Google Scholar 

  18. Caldwell, H. D., Kuo, C. C., Kenny, G. E.: Antigenic analysis of chlamydiae by two-dimensional immuno-electrophoresis. I. Antigenic heterogeneity betweenChlamydia trachomatis andChlamydia psittaci. Journal of Immunology 1975, 115: 963–968.

    Google Scholar 

  19. Lowry, O. H., Rosebrough, N. J., Farr, A. L., Randall, R. S.: Protein measurements with the folin-phenol reagent. Journal of Biological Chemistry 1951, 193: 265–274.

    PubMed  Google Scholar 

  20. Schmitz, H., Doerr, H. W., Kampa, D., Vogt, A.: Solid-phase enzyme immunoassay for immunoglobulin M antibodies to cytomegalovirus. Journal of Clinical Microbiology 1977, 5: 629–634.

    PubMed  Google Scholar 

  21. Plotz, C. M., Singer, J. M.: The latex fixation test. II. Results in rheumatoid arthritis. American Journal of Medicine 1956, 21: 893–896.

    Article  PubMed  Google Scholar 

  22. Walls, K. W., Bullock, S. L., English, D. K.: Use of the enzyme-linked immunosorbent assay and its microadaption for the serodiagnosis of toxoplasmosis. Journal of Clinical Microbiology 1977, 5: 273–277.

    PubMed  Google Scholar 

  23. Woodward, B. C.: Evaluation of an enzymelinked immunosorbent assay specific for human immunoglobulin G as a screening test for detecting anti-toxoplasma antibodies. Journal of Clinical Microbiology 1982, 16: 367–372.

    PubMed  Google Scholar 

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Authors and Affiliations

  1. Institut National de la Santé et de la Recherche Médicale U.43, Hôpital St. Vincent de Paul, 74 Avenue Denfert Rochereau, F-75014, Paris

    P. Duc-Goiran, J. Raymond & J. B. Léauté

  2. CHU d'Amiens, Place Victor Pauchet, B.P. 363, F-80000, Amiens

    J. Orfila

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  1. P. Duc-Goiran
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  2. J. Raymond
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  3. J. B. Léauté
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  4. J. Orfila
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Duc-Goiran, P., Raymond, J., Léauté, J.B. et al. Use of the enzyme-linked immunosorbent assay for detection of antibodies toChlamydia trachomatis . Eur. J, Clin. Microbiol. 2, 32–38 (1983). https://doi.org/10.1007/BF02019920

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