Abstract
Radiometric detection of facultative gram-positive cocci, particularly streptococci, has been reported to be suboptimal. A recently marketed anaerobic Bactec bottle (7C) with purportedly improved capability to detect facultative gram-positive cocci in blood was compared with the previously available bottle in a study of simulated bacteremia. The Bactec system detected all betahemolytic streptococci and enterococci tested. Detection of alpha-hemolytic streptococci was improved in general. However, detection ofStreptococcus mutans, Streptococcus bovis andStreptococcus pneumoniae remained suboptimal under anaerobic conditions. A metabolic index unit threshold for positivity of 15 is recommended when employing the anaerobic Bactec bottle.
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Finegold, S. M., White, M. L., Ziment, I., Winn, W. R.: Rapid diagnosis of bacteremia. Applied Microbiology 1969, 18: 458–453.
Hall, M., Warren, E., Washington, J. A.: Detection of bacteremia with liquid media containing sodium polyanethol-sulfonate. Applied Microbiology 1974, 27: 187–191.
Randall, E. L.: Long-term evaluation of a system for radiometric detection of bacteremia. In: Schlessinger, D. (ed.): Microbiology-1975. American Society for Microbiology, Washington D.C., 1975.
Sonnenwirth, A. C.: Bacteremia — extent of the problem. In: Sonnenwirth, A. C. (ed.): Bacteremia — laboratory and clinical aspects. Charles C. Thomas, Springfield, 1973.
Washington, J. A.: Comparison of two commercially available media for detection of bacteremia. Applied Microbiology 1971, 22: 604–607.
Kiani, D., Quinn, E. L., Burch, K. H., Madhavan, T., Saravolatz, L. D., Neblett, T. R.: The increasing importance of polymicrobial bacteremia. Journal of the American Medical Association 1979, 242: 1044–1047.
Reik, H., Rubin, S. J.: Evaluation of the buffy coat smear for rapid detection of bacteremia. Journal of the American Medical Association 1981, 245: 357–359.
Werner, A. S., Cobbs, C. G., Kaye, D., Hook, E. W.: Studies on the bacteremia of bacterial endocarditis. Journal of the American Medical Association 1967, 202: 199–203.
Strauss, R. R., Throm, R., Friedman, H.: Radiometric detection of bacteremia: requirements for terminal subcultures. Journal of Clinical Microbiology 1977, 5: 145–148.
Beckwith, D. G.: Detection of group D and viridans streptococci in blood by radiometric methods. Journal of Clinical Microbiology 1979, 9: 20–22.
Facklam, R. R.: Physiological differentiation of viridans streptococci. Journal of Clinical Microbiology 1977, 5: 185–201.
Facklam, R. R.: Streptococci and aerococci. In: Lenette, E. H., Balows, A., Hausler, W. J., Truant, J. P. (ed.): Manual of clinical microbiology. American Society for Microbiology, Washington D.C., 1980.
Kloos, W. E., Smith, P. B.: Staphylococci. In: Lennette, E. H., Balows, A., Hausler, W. J., Truant, J. P. (ed.): Manual of clinical microbiology. American Society for Microbiology, Washington D.C., 1980.
Rosner, R.: A quantitative evaluation of three different blood culture systems. In: Sonnenwirth, A. C. (ed.): Bacteremia — clinical and laboratory aspects. Charles C. Thomas, Springfield, 1973.
Adeniyi-Jones, C. C., Stevens, D. L., Rasquinha, E. S.: False no-growth blood cultures in pneumococcal pneumonia. Journal of Clinical Microbiology 1980, 12: 572–575.
Gross, D. C., Houghton, M. P., Roberts, R. B.: Evaluation of blood culture media for isolation of pyridoxal-dependentStreptococcus mitior (mitis). Journal of Clinical Microbiology 1981, 14: 266–272.
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Beckwith, D.G., Conyers, W.C. & Etowski, D.C. Anaerobic radiometric detection of facultative gram-positive cocci in blood. Eur. J, Clin. Microbiol. 1, 212–216 (1982). https://doi.org/10.1007/BF02019710
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DOI: https://doi.org/10.1007/BF02019710