Skip to main content
Log in

Anaerobic radiometric detection of facultative gram-positive cocci in blood

  • Original Articles
  • Published:
European Journal of Clinical Microbiology Aims and scope Submit manuscript

Abstract

Radiometric detection of facultative gram-positive cocci, particularly streptococci, has been reported to be suboptimal. A recently marketed anaerobic Bactec bottle (7C) with purportedly improved capability to detect facultative gram-positive cocci in blood was compared with the previously available bottle in a study of simulated bacteremia. The Bactec system detected all betahemolytic streptococci and enterococci tested. Detection of alpha-hemolytic streptococci was improved in general. However, detection ofStreptococcus mutans, Streptococcus bovis andStreptococcus pneumoniae remained suboptimal under anaerobic conditions. A metabolic index unit threshold for positivity of 15 is recommended when employing the anaerobic Bactec bottle.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  1. Finegold, S. M., White, M. L., Ziment, I., Winn, W. R.: Rapid diagnosis of bacteremia. Applied Microbiology 1969, 18: 458–453.

    PubMed  Google Scholar 

  2. Hall, M., Warren, E., Washington, J. A.: Detection of bacteremia with liquid media containing sodium polyanethol-sulfonate. Applied Microbiology 1974, 27: 187–191.

    PubMed  Google Scholar 

  3. Randall, E. L.: Long-term evaluation of a system for radiometric detection of bacteremia. In: Schlessinger, D. (ed.): Microbiology-1975. American Society for Microbiology, Washington D.C., 1975.

    Google Scholar 

  4. Sonnenwirth, A. C.: Bacteremia — extent of the problem. In: Sonnenwirth, A. C. (ed.): Bacteremia — laboratory and clinical aspects. Charles C. Thomas, Springfield, 1973.

    Google Scholar 

  5. Washington, J. A.: Comparison of two commercially available media for detection of bacteremia. Applied Microbiology 1971, 22: 604–607.

    PubMed  Google Scholar 

  6. Kiani, D., Quinn, E. L., Burch, K. H., Madhavan, T., Saravolatz, L. D., Neblett, T. R.: The increasing importance of polymicrobial bacteremia. Journal of the American Medical Association 1979, 242: 1044–1047.

    PubMed  Google Scholar 

  7. Reik, H., Rubin, S. J.: Evaluation of the buffy coat smear for rapid detection of bacteremia. Journal of the American Medical Association 1981, 245: 357–359.

    PubMed  Google Scholar 

  8. Werner, A. S., Cobbs, C. G., Kaye, D., Hook, E. W.: Studies on the bacteremia of bacterial endocarditis. Journal of the American Medical Association 1967, 202: 199–203.

    PubMed  Google Scholar 

  9. Strauss, R. R., Throm, R., Friedman, H.: Radiometric detection of bacteremia: requirements for terminal subcultures. Journal of Clinical Microbiology 1977, 5: 145–148.

    PubMed  Google Scholar 

  10. Beckwith, D. G.: Detection of group D and viridans streptococci in blood by radiometric methods. Journal of Clinical Microbiology 1979, 9: 20–22.

    PubMed  Google Scholar 

  11. Facklam, R. R.: Physiological differentiation of viridans streptococci. Journal of Clinical Microbiology 1977, 5: 185–201.

    Google Scholar 

  12. Facklam, R. R.: Streptococci and aerococci. In: Lenette, E. H., Balows, A., Hausler, W. J., Truant, J. P. (ed.): Manual of clinical microbiology. American Society for Microbiology, Washington D.C., 1980.

    Google Scholar 

  13. Kloos, W. E., Smith, P. B.: Staphylococci. In: Lennette, E. H., Balows, A., Hausler, W. J., Truant, J. P. (ed.): Manual of clinical microbiology. American Society for Microbiology, Washington D.C., 1980.

    Google Scholar 

  14. Rosner, R.: A quantitative evaluation of three different blood culture systems. In: Sonnenwirth, A. C. (ed.): Bacteremia — clinical and laboratory aspects. Charles C. Thomas, Springfield, 1973.

    Google Scholar 

  15. Adeniyi-Jones, C. C., Stevens, D. L., Rasquinha, E. S.: False no-growth blood cultures in pneumococcal pneumonia. Journal of Clinical Microbiology 1980, 12: 572–575.

    PubMed  Google Scholar 

  16. Gross, D. C., Houghton, M. P., Roberts, R. B.: Evaluation of blood culture media for isolation of pyridoxal-dependentStreptococcus mitior (mitis). Journal of Clinical Microbiology 1981, 14: 266–272.

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Beckwith, D.G., Conyers, W.C. & Etowski, D.C. Anaerobic radiometric detection of facultative gram-positive cocci in blood. Eur. J, Clin. Microbiol. 1, 212–216 (1982). https://doi.org/10.1007/BF02019710

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02019710

Keywords

Navigation