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European Journal of Clinical Microbiology

, Volume 2, Issue 4, pp 327–334 | Cite as

Antibiotic-induced modification ofBacteroides fragilis and its susceptibility to phagocytosis by human polymorphonuclear leukocytes

  • C. G. Gemmell
  • P. K. Peterson
  • D. Schmeling
  • J. Mathews
  • P. G. Quie
Originals Articles

Abstract

Bacteroides fragilis grown in the presence of sub-inhibitory concentrations of clindamycin was shown to be altered its degree of encapsulation and susceptibility to phagocytosis by human polymorphonuclear leukocytes. Little polysaccharide capsule could be demonstrated either by light or transmission electron microscopy when the bacteria were grown anaerobically for four hours in the presence of 1/2 MIC of clindamycin. Such clindamycin-grown cells could be opsonized by normal human serum, and although less complement was consumed in the process, were more effectively taken up by the leukocytes than bacteria grown in the absence of the drug (45 % versus 24 %). It was also shown that drug treatment caused significant cellular leakage in the presence of serum, the3H-label appearing extracellularly. In addition there was greater loss of viability of the bacterial cells grown in the presence of the drug and subsequently exposed to the leukocytes for 60 min.

Keywords

Electron Microscopy Transmission Electron Microscopy Internal Medicine Polysaccharide Drug Treatment 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1983

Authors and Affiliations

  • C. G. Gemmell
    • 1
  • P. K. Peterson
    • 2
  • D. Schmeling
    • 1
  • J. Mathews
    • 3
  • P. G. Quie
    • 2
  1. 1.Department of BacteriologyUniversity of Glasgow, Royal InfirmaryGlasgowUK
  2. 2.Departments of Pediatrics and MedicineUniversity of MinnesotaMinneapolisUSA
  3. 3.Upjohn International Inc.KalamazooUSA

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