Abstract
A blood-free medium for the recovery of thermophilic Campylobacter was compared with Butzler Medium Virion during a one-year study using 2,893 human feces samples. Ninety Campylobacter strains (3.1 %) were isolated after incubation for 48 h at 42 ‡C in a candle jar atmosphere. Three strains of Campylobacter jejuni were isolated on the blood-free medium only and one on Butzler Medium Virion only. Fecal flora was equally well inhibited on both media except for gram-positive organisms, which were completely inhibited only on the blood-free medium.
Similar content being viewed by others
References
Blaser, M. J., Berkowitz, I. D., LaForce, F. M., Cravens, J., Reller, L. B., Wang, W. L.: Campylobacter enteritis: clinical and epidemiological features. Annals of Internal Medicine 1979, 91: 179–185.
Butzler, J. P., Skirrow, M. B.: Campylobacter enteritis. Clinical Gastroenterology 1979, 8: 737–765.
Skirrow, M. B.: Campylobacter enteritis: a “new” disease. British Medical Journal 1977, ii: 9–11.
Bolton, F. J., Robertson, L.: A selective medium for isolatingCampylobacter jejuni/coli. Journal of Clinical Pathology 1982, 35: 462–467.
Karmali, M. A., Simor, A. E., Roscoe, M., Fleming, P. C., Smith, S. S., Lane, J.: Evaluation of a blood-free, charcoal-based, selective medium for the isolation of campylobacter organisms from feces. Journal of Clinical Microbiology 1986, 23: 456–459.
George, H. A., Huffman, P. S., Smibert, R. M., Krieg, N. R.: Improved media for growth and aerotolerance ofCampylobacter fetus. Journal of Clinical Microbiology 1978, 8: 36–41.
Huffman, P. S., George, H. A., Krieg, N. R., Smibert, R. M.: Studies on the microaerophilic nature ofCampylobacter fetus subsp.jejuni. II. Role of exogenous superoxide anions and hydrogen peroxide. Canadian Journal of Microbiology 1979, 25: 8–16.
Goossens, H., DeBoeck, M., Butzler, J. P.: A new selective medium for the isolation ofCampylobacter fetus subsp.jejuni from human feces. European Journal of Clinical Microbiology 1983, 2: 389–394.
Goossens, H., De Boeck, M., Van Landuyt, H. W., Butzler, J. P.: Isolation ofCampylobacter jejuni from human feces. In: Butzler, J. P. (ed.): Campylobacter infection in man and animals. CRC Press, Boca Raton, FA, 1984, p. 39–50.
Morris, G. K., Patton, C. M.:Campylobacter. In: Lennette, E. H., Balows, A., Hausler, W. J., Shadomy, H. J. (ed.): Manual of clinical microbiology. American Society for Microbiology, Washington, DC, 1985, p. 302–308.
Grant, I. H., Richardson, N. J., Bokkenheuser, V. D.: Broiler chickens as potential source of Campylobacter infections in humans. Journal of Clinical Microbiology 1980, 11: 508–510.
Lai-King, N. G., Stiles, M. E., Taylor, D. E.: Comparison of basal media for culturingCampylobacter jejuni andCampylobacter coli. Journal of Clinical Microbiology 1985, 21: 226–229.
Lai-King, N. G., Stiles, M. E., Taylor, D. E.: Inhibition ofCampylobacter coli andCampylobacter jejuni by antibiotics used in selective growth media. Journal of Clinical Microbiology 1985, 22: 510–514.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Van Landuyt, H.W., Fossépré, J.M. & Gordts, B. A blood-free medium for isolation of thermophilicCampylobacter species. Eur. J, Clin. Microbiol. 6, 201–203 (1987). https://doi.org/10.1007/BF02018214
Issue Date:
DOI: https://doi.org/10.1007/BF02018214