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European Journal of Clinical Microbiology

, Volume 6, Issue 2, pp 147–151 | Cite as

Ability of enzyme-linked immunosorbent assays to detect early immunoglobulin G antibodies toToxoplasma gondii

  • C. Verhofstede
  • L. Sabbe
  • L. Van Renterghem
Articles

Abstract

Two ELISA procedures, one using sonicated antigen coated with carbonate buffer and the other formalin fixed trophozoites with dry coating, differ in their ability to detect early antibodies in toxoplasmosis. In order to identify factors responsible for this difference, seven ELISA systems differing from each other in antigen used and/or coating procedure were compared. Both fixation of the trophozoites with formalin and air-drying of the antigen in the microtiterplate were important factors determining the ability of the assay to detect IgG antibodies in the early stage of infection. Differences in the results of the two ELISA procedures can be used to distinguish between the acute and chronic stages of infection.

Keywords

Internal Medicine Formalin Immunosorbent Assay Toxoplasmosis Carbonate Buffer 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1987

Authors and Affiliations

  • C. Verhofstede
    • 1
  • L. Sabbe
    • 1
  • L. Van Renterghem
    • 1
  1. 1.Department of Medical MicrobiologyUniversity HospitalGhentBelgium

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