European Journal of Clinical Microbiology

, Volume 6, Issue 2, pp 147–151 | Cite as

Ability of enzyme-linked immunosorbent assays to detect early immunoglobulin G antibodies toToxoplasma gondii

  • C. Verhofstede
  • L. Sabbe
  • L. Van Renterghem


Two ELISA procedures, one using sonicated antigen coated with carbonate buffer and the other formalin fixed trophozoites with dry coating, differ in their ability to detect early antibodies in toxoplasmosis. In order to identify factors responsible for this difference, seven ELISA systems differing from each other in antigen used and/or coating procedure were compared. Both fixation of the trophozoites with formalin and air-drying of the antigen in the microtiterplate were important factors determining the ability of the assay to detect IgG antibodies in the early stage of infection. Differences in the results of the two ELISA procedures can be used to distinguish between the acute and chronic stages of infection.


Internal Medicine Formalin Immunosorbent Assay Toxoplasmosis Carbonate Buffer 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Voller, A., Bidwell, D. E., Bartlett, A., Fleck, D. G., Perkins, M., Dadehin, B.: A microplate enzymeimmunoassay for toxoplasma antibody. Journal of Clinical Pathology 1976, 29: 150–153.PubMedGoogle Scholar
  2. 2.
    Walls, K. W., Bullock, S. L., English, D. K.: Enzyme-linked immunosorbent assay and its microadaption for the serodiagnosis of toxoplasmosis. Journal of Clinical Microbiology 1977, 5: 273–277.PubMedGoogle Scholar
  3. 3.
    Camargo, M. E., Ferreira, A. W., Mineo, J. R., Takiguti, C. K., Nakahara, C. S.: Immunoglobulin G and immunoglobulin M enzyme-linked immunosorbent assay and defined toxoplasmosis serological patterns. Infection and Immunity 1978, 21: 55–58.PubMedGoogle Scholar
  4. 4.
    Lin, T. M., Halbert, S. P., O'Connor, G. R.: Standardized quantitative enzyme-linked immunoassay for antibodies toToxoplasma gondii. Journal of Clinical Microbiology 1980, 11: 675–681.PubMedGoogle Scholar
  5. 5.
    Van Loon, A. M., Van Der Veen, J.: Enzyme-linked immunosorbent assay for quantitation of toxoplasma antibodies in human sera. Journal of Clinical Pathology 1980, 33: 635–639.PubMedGoogle Scholar
  6. 6.
    Woodward, B. C.: Evaluation of an enzyme-linked immunosorbent assay specific for human immunoglobulin G as a screening test for detecting anti-toxoplasma antibodies. Journal of Clinical Microbiology 1982, 16: 367–372.PubMedGoogle Scholar
  7. 7.
    Violand, S. A., Mitchell, T. G., Kleeman, K. T.: Comparison of an enzyme-linked immunoassay and a quantitative indirect fluorescent-antibody test with the conventional indirect fluorescent-antibody test for detecting antibodies toToxoplasma gondii. Journal of Clinical Microbiology 1982, 16: 341–344.PubMedGoogle Scholar
  8. 8.
    Picher, O., Aspöck, H., Auer, H., Hermentin, K.: Enzyme-linked immunosorbent assay with whole trophozoites ofToxoplasma gondii. Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene (A) 1982, 253: 397–401.Google Scholar
  9. 9.
    Van Knapen, F.: Immunodiagnosis of toxoplasmosis. Thesis, University of Amsterdam, 1984.Google Scholar
  10. 10.
    Braveny, I., Winter, W., Disko, R.: A method of mass cultivation ofToxoplasma gondii in cell culture. Tropical Medicine and Parasitology 1978, 29: 432–434.Google Scholar
  11. 11.
    Naot, Y., Guptill, D. R., Mullenax, J., Remington, J. S.: Characterization ofToxoplasma gondii antigens that react with human immunoglobulin M and immunoglobulin G antibodies. Infection and Immunity 1983, 41: 331–338.PubMedGoogle Scholar
  12. 12.
    Lew, A. M.: The effect of epitope density and antibody affinity on the ELISA as analysed by monoclonal antibodies. Journal of Immunological Methods 1984, 72: 171–176.PubMedGoogle Scholar
  13. 13.
    Desmonts, G., Remington, J. S.: Direct agglutination test for diagnosis of toxoplasma infection: method for increasing sensitivity and specificity. Journal of Clinical Microbiology 1980, 11: 562–568.PubMedGoogle Scholar
  14. 14.
    Pettersen, E. K.: Recency ofToxoplasma gondii infections correlated with results obtained in dye test and enzyme-linked immunosorbent assay. Acta Pathologica, Microbiologica et Immunologica Scandinavica (B) 1981, 89: 407–410.Google Scholar

Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1987

Authors and Affiliations

  • C. Verhofstede
    • 1
  • L. Sabbe
    • 1
  • L. Van Renterghem
    • 1
  1. 1.Department of Medical MicrobiologyUniversity HospitalGhentBelgium

Personalised recommendations