Automated bacteriuria screening using the Berthold LB 950 luminescence analyser
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The Berthold LB950 Automatic Luminescence Analyser was used to estimate bacterial adenosine triphosphate in urine. The system provided a rapid (15min) and fully automated screening test for bacteriuria at the 105CFU/ml level. Bioluminescence results for 1040 urines were compared with viable counts using two reference culture methods and frequency distributions of bacterial counts and adenosine triphosphate levels were calculated. With a specificity of 79% the automated method showed a sensitivity of 84% using a pour plate reference count and 91 % using a standard loop reference count. When contaminated urines were excluded the sensitivity improved to 98%. The automated bioluminescence test, though expensive, was shown to work well with good quality specimens.
KeywordsScreening Test Culture Method Automate Method Bacterial Count Bacteriuria
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- 1.Chappelle, E. W., Picciolo, G. L.: Bacterial adenosine triphosphate (ATP) as a measure of urinary tract infection. NASA publication TSPI-10051. 1971. Technology Utilization Office, Goddard Space Flight Center. National Aeronautics and Space Administration, Greenbelt, MD, USA.Google Scholar
- 10.Johnston, H. H., Curtis, G. D. W.: Detection of bacteria by bioluminescence — problems in removal of non-bacterial ATP. In: Schram, E., Stanley, P. (ed.): International symposium on analytical applications of bioluminescence and chemiluminescence. State Printing and Publishing, Inc., Westlake Village, California, 1979, p. 446–447.Google Scholar
- 11.Galen, R. S., Gambino, S. R.: Beyond normality, the predictive value and efficiency of medical diagnoses. John Wiley and Sons, Inc., New York, 1975, p. 13.Google Scholar
- 15.Nichols, W. W., Curtis, G. D. W., Johnston, H. H.: Analysis of the disagreement between automated bioluminescence-based and culture methods for detecting significant bacteriuria with proposals for standardising evaluations of bacteriuria detection methods. Journal of Clinical Microbiology 1982, 15: 802–809.PubMedGoogle Scholar