European Journal of Clinical Microbiology

, Volume 6, Issue 3, pp 275–280 | Cite as

Automated bacteriuria screening using the Berthold LB 950 luminescence analyser

  • G. D. W. Curtis
  • H. H. Johnston
  • A. R. Hack
Article

Abstract

The Berthold LB950 Automatic Luminescence Analyser was used to estimate bacterial adenosine triphosphate in urine. The system provided a rapid (15min) and fully automated screening test for bacteriuria at the 105CFU/ml level. Bioluminescence results for 1040 urines were compared with viable counts using two reference culture methods and frequency distributions of bacterial counts and adenosine triphosphate levels were calculated. With a specificity of 79% the automated method showed a sensitivity of 84% using a pour plate reference count and 91 % using a standard loop reference count. When contaminated urines were excluded the sensitivity improved to 98%. The automated bioluminescence test, though expensive, was shown to work well with good quality specimens.

Keywords

Screening Test Culture Method Automate Method Bacterial Count Bacteriuria 

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Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1987

Authors and Affiliations

  • G. D. W. Curtis
    • 1
  • H. H. Johnston
    • 1
  • A. R. Hack
    • 1
  1. 1.Bacteriology Department and Regional Public Health LaboratoryJohn Radcliffe HospitalOxfordUK

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