Calcified Tissue Research

, Volume 5, Issue 1, pp 85–90 | Cite as

Cytochrome oxidase activities of various cartilage tissues during growth and development

  • Albert S. Fine
  • Philip Person
Short Communications


As rabbits increase in size from 165 grams to 2,205 grams body weight, cytochrome oxidase activity of epiphyseal cartilage homogenates does not change in manometric and spectrophotometric enzyme assays, whereas the oxidase activities of xiphisternal and ear cartilage homogenates decline. Light absorption spectra of cartilage tissues were made for the first time, using strips of rabbit ear and pieces of chick embryo cartilage. Such spectra reveal characteristic absorption maxima for the terminal respiratory complex of mitochondria, including b, c and oxidase components and flavoprotein.

Key words

Cartilage Electron transport Cytochrome oxidase 


Lorsque des lapins augmentent pondéralement de 165 à 2,205 grammes, l'activité en cytochrome oxydase de cartilage épiphysaire homogénéisé n'est pas modifiée, en utilisant des tests enzymatiques manométriques et spectrophotométriques; alors que l'activité en oxydase du cartilage xyphoide du sternum et de l'oreille homogénéisé décroit. Des spectres d'absorption optique des tissus cartilagineux, réalisés pour la première fois à l'aide de fragments d'oreilles de lapin et de cartilage embryonnaire de poulets, montrent un maximum d'absorption caractéristique pour le système respiratoire final des mitochondries, y compris les composés b, c et oxydasiques ainsi que les flavoprotéines.


Während Kaninchen ihr Körpergewicht von 165 auf 2205 g erhöhen, erfolgt keine Veränderung in der Cytochrom-Oxydase-Aktivität der epiphysären Knorpel-Homogenate in manometrischen und spectrophotometrischen Enzymversuchen, jedoch vermindern sich die Oxydase-Aktivitäten von xiphisternalen und von Ohrknorpel-Homogenaten. Lichtabsorptionsspektren von Knorpelgewebe wurden zum ersten Mal gemacht, indem Streifen von Kaninchenohrstücken und Stücke von Kükenembryoknorpel verwendet wurden. Solche Spektren zeigen charakteristische Absorptionsmaxima für den terminalen Atmungskomplex der Mitochondrien, einschließlich b, c und Oxydasekomponenten sowie Flavoprotein.


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  1. Barbieri, E., Ronchetti, G. C.: Adenosine triphosphate in the metaphyseal cartilage during skeletal growth as compared with other tissues. Atti Soc. lombarda Sci. med.-Biol.13, 321–324 (1958).Google Scholar
  2. Bassett, C. A.: Environmental and cellular factors regulating osteogenesis. In: Bone biodynamics (H. M. Frost, ed.), p. 233–244. Boston: Little, Brown & Co. 1964.Google Scholar
  3. Eichel, B., Wainio, W. W., Person, P., Cooperstein, S. J.: Partial separation and characterization of cytochrome oxidase and cytochrome b. J. biol. Chem.183, 89–103 (1950).Google Scholar
  4. Fang, M., Rasmussen, H.: Parathyroid hormone and mitochondrial respiration. Endocrinology75, 434–445 (1964).PubMedGoogle Scholar
  5. Fine, A., Person, P.: Terminal respiration of cartilage tissues. Biochim. biophys. Acta (Amst.)78, 729–732 (1963).Google Scholar
  6. Kirk, J. E., From Hansen, P.: Metabolism of avascularized tissues and changes associated with ageing. In: Cowdry's problems of ageing, 3rd edit. (A. I. Lansing, ed), p. 730–763. Baltimore: Williams & Wilkins Co. 1952.Google Scholar
  7. Lowry, O., Rosenbrough, N. J., Farr, A. L., Randall, R. J.: Protein measurements with Folin phenol reagent. J. biol. Chem.193, 265–273 (1951).PubMedGoogle Scholar
  8. Person, P., Fine, A.: cytochrome oxidase and succinoxidase activity ofLimulus gill cartilage. Arch. Biochem.84, 123–133 (1959).PubMedGoogle Scholar
  9. ——: Multiple reflectance filter paper spectrophotometry of microvolumes of turbid cytochrome preparations. Biochim. biophys. Acta (Amst.)90, 168–170 (1964).Google Scholar
  10. — Wainio, W. W., Eichel, B.: The prosthetic groups of cytochrome oxidase and cytochrome b. J. biol. Chem.202, 369–381 (1953).PubMedGoogle Scholar
  11. Rasmussen, H.: Mitochondrial ion transport: mechanisms and physiological significance. Fed. Proc.25, 903–911 (1966).PubMedGoogle Scholar
  12. Stern, B., Glimcher, M. J., Goldhaber, P.: The effect of various oxygen tensions on the synthesis and degradation of bone collagen in tissue culture. Proc. Soc. exp. Biol. (N.Y.)121, 869–872 (1966).Google Scholar

Copyright information

© Springer-Verlag 1970

Authors and Affiliations

  • Albert S. Fine
    • 1
  • Philip Person
    • 1
  1. 1.Special Research Laboratory for Oral Tissue MetabolismVeterans Administration HospitalBrooklynU.S.A.

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