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Calcified Tissue Research

, Volume 5, Issue 1, pp 85–90 | Cite as

Cytochrome oxidase activities of various cartilage tissues during growth and development

  • Albert S. Fine
  • Philip Person
Short Communications

Abstract

As rabbits increase in size from 165 grams to 2,205 grams body weight, cytochrome oxidase activity of epiphyseal cartilage homogenates does not change in manometric and spectrophotometric enzyme assays, whereas the oxidase activities of xiphisternal and ear cartilage homogenates decline. Light absorption spectra of cartilage tissues were made for the first time, using strips of rabbit ear and pieces of chick embryo cartilage. Such spectra reveal characteristic absorption maxima for the terminal respiratory complex of mitochondria, including b, c and oxidase components and flavoprotein.

Key words

Cartilage Electron transport Cytochrome oxidase 

Résumé

Lorsque des lapins augmentent pondéralement de 165 à 2,205 grammes, l'activité en cytochrome oxydase de cartilage épiphysaire homogénéisé n'est pas modifiée, en utilisant des tests enzymatiques manométriques et spectrophotométriques; alors que l'activité en oxydase du cartilage xyphoide du sternum et de l'oreille homogénéisé décroit. Des spectres d'absorption optique des tissus cartilagineux, réalisés pour la première fois à l'aide de fragments d'oreilles de lapin et de cartilage embryonnaire de poulets, montrent un maximum d'absorption caractéristique pour le système respiratoire final des mitochondries, y compris les composés b, c et oxydasiques ainsi que les flavoprotéines.

Zusammenfassung

Während Kaninchen ihr Körpergewicht von 165 auf 2205 g erhöhen, erfolgt keine Veränderung in der Cytochrom-Oxydase-Aktivität der epiphysären Knorpel-Homogenate in manometrischen und spectrophotometrischen Enzymversuchen, jedoch vermindern sich die Oxydase-Aktivitäten von xiphisternalen und von Ohrknorpel-Homogenaten. Lichtabsorptionsspektren von Knorpelgewebe wurden zum ersten Mal gemacht, indem Streifen von Kaninchenohrstücken und Stücke von Kükenembryoknorpel verwendet wurden. Solche Spektren zeigen charakteristische Absorptionsmaxima für den terminalen Atmungskomplex der Mitochondrien, einschließlich b, c und Oxydasekomponenten sowie Flavoprotein.

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Copyright information

© Springer-Verlag 1970

Authors and Affiliations

  • Albert S. Fine
    • 1
  • Philip Person
    • 1
  1. 1.Special Research Laboratory for Oral Tissue MetabolismVeterans Administration HospitalBrooklynU.S.A.

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