Calcified Tissue Research

, Volume 5, Issue 1, pp 49–55 | Cite as

Parathyroid hormone as a stimulus to mast cell accumulation in bone

  • S. David Rockoff
  • John D. ArmstrongJr.
Original Papers

Abstract

Low doses of parathyroid hormone were administered over a 30-day period to Sprague-Dawley weanlings rats. In 7 of 12 animals, mast cell hyperplasia occurred in the proximal tibial metaphyseal marrow, without an associated fall in serum phosphorus or rise in serum calcium. Further, no alteration occurred in body weight or skeletal ash weight relative to the control population. A third group, on a calcium-deficient diet containing adequate vitamin D, showed marked bone marrow mast cell hyperplasia in all animals.

Key words

Bone Marrow Mast cell Parathyroid Calcium Deficiency 

Résumé

Des doses faibles d'hormone parathyroidienne sont administrées pendant 30 jours à des rats sevrés de l'espèce Sprague-Dawley. Chez 7 des 12 animaux utilisés, une hyperplasie des mastocytes est observée dans la moëlle tibiale métaphysaire proximale, sans chute associée du phosphore sérique ou élévation du calcium sérique. En outre, aucun changement de poids corporel ou du poids des cendres squelettiques n'est observé par rapport aux témoins. Un troisième groupe, soumis à une alimentation pauvre en calcium, mais contenant une quantité suffisante de vitamine D, présente une hyperplasie mastocytaire marquée au niveau de la moëlle osseuse de tous les animaux.

Zusammenfassung

Entwöhnte Sprague-Dawley-Ratten erhiclten während einer Periode von 30 Tagen niedrige Dosen von Parathyroidhormon. 7 von 12 Tieren zeigten eine Mastzell-Hyperplasie im proximalen Teil des Tibiametaphysenmarks, ohne gleichzeitige Erniedrigung des Serumphosphates oder Anstieg des Serumcalciums. Femer traten im Vergleich zur Kontrollgruppe keine Veränderungen im Körpergewicht oder im Gewicht des Aschenrückstandes des Skelets auf. Eine dritte Gruppe, die eine Calcium-arme, entsprechend mit Vitamin D angereicherte Nahrung erhielt, zeigte eine ausgeprägte Mastzell-Hyperplasie im Knochenmark aller Tiere.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Asher, J. D., Nichols, G., Jr.: Heparin stimulation of bone collagenase activity (abstract). Fed. Proc.24, 211 (1965).Google Scholar
  2. Bordier, P.: Discussion of paper by B. Frame (1969).Google Scholar
  3. Burkhart, J. M., Jowsey, J.: Parathyroid and thyroid hormones in the development of immobilization osteoporosis. Endocrinology81, 1053–1062 (1967).PubMedGoogle Scholar
  4. Frame, B.: Bone marrow factors in osteoporosis. Presented at the Internat. Symposium on Osteoporosis, June 26, 1969, Montifiore Hospital, New York City.Google Scholar
  5. —, Nixon, R. K.: Bone marrow mast cells in osteoporosis of aging. New Engl. J. Med.279, 626–631 (1968).PubMedGoogle Scholar
  6. Goldhaber, P.: Heparin enhancement of factors stimulating bone resorption in tissue culture. Science147, 407–408 (1965a).PubMedGoogle Scholar
  7. Goldhaber, P.: Bone resorption factors, cofactors, and giant vacuole osteoclasts in tissue culture. In: The Parathyroid glands: Ultrastructure, secretion and function, ed. P. J. Gaillard, R. V. Talmage and A. M. Budy, p. 153–171. Chicago: Univ. Chicago Press 1965b.Google Scholar
  8. Griffith, G. C., Nichols, G., Jr., Asher, J. D., Flanagan, B.: Heparin osteoporosis. J. Amer. med. Ass.193, 91–94 (1965).Google Scholar
  9. Harrison, M., Fraser, R.: The parathyroid glands and calcium-deficiency in the rat. J. Endocr.21, 207–211 (1960).PubMedGoogle Scholar
  10. Jaffe, M. D., Willis, P. W., III: Multiple fractures associated with longterm sodium heparin therapy. J. Amer. med. Ass.193, 158–160 (1965).Google Scholar
  11. Lascano, E. F.: Mast cells in human tumors. Cancer (Philad.)11, 1110–1114 (1958).Google Scholar
  12. Lindholm, R., Lindholm, S., Liukko, P.: Fracture healing and mast cells. I. The periosteal callus in rats. Acta orthop. scand.38, 115–122 (1967).PubMedGoogle Scholar
  13. ———, Paasimäki, J., Isokääntä, S., Rossi, S., Autio, E., Tamminen, E.: The mast cell as a component of callus in healing fractures. J. Bone Surg.51B, 148–155 (1969).Google Scholar
  14. Miller, R. G.: Simultaneous statistical inference, p. 81–91. New York: McGraw-Hill 1966.Google Scholar
  15. Miller, W. E., deWolfe, V. G.: Osteoporosis resulting from heparin therapy. Report of a case. Cleveland Clin. Quart.33, 31–34 (1966).Google Scholar
  16. Pak, C. Y. C., Zisman, E., Evens, R., Jowsey, J., Delea, C. S., Bartter, F. C. Treatment of osteoporosis with calcium infusions. Amer. J. Med.47, 7–22 (1969).PubMedGoogle Scholar
  17. Riley, J. F.: Functional significance of histamine and heparin in tissue mast cells. In: Mast cells and basophils, ed. J. Padwer, p. 151–163. New York: Academy of Sciences 1963.Google Scholar
  18. Robertis, E. de: The cytology of the parathyroid and thyroid glands of rats with experimental rickets. Anat. Rec.79, 417–433 (1941).Google Scholar
  19. Rockoff, S. D., Bravo, A., Kaye, H., Spencer, R. P.: Rapid increase in fraction of cardiac output to bone in experimental calcium deficiency. Calc. Tiss. Res.3, 17–29 (1969).Google Scholar
  20. Severson, A. R.: Mast cells in areas of experimental bone resorption and remodelling. Brit. J. exp. Path.50, 17–21 (1969).PubMedGoogle Scholar
  21. Stoerk, H. C., Carnes, W. H.: The relation of the dietary Ca: P ratio to serum Ca and to parathyroid volume. J. Nutr.29, 43–50 (1945).Google Scholar
  22. Urist, M. R., McLean, F. C.: Accumulation of mast cells in endosteum of bones of calciumdeficient rats. Arch. Path.63, 239–251 (1957).Google Scholar
  23. White, W. L., Erickson, M. M., Stevens, S. C.: Practical automation for the clinical laboratory. St. Louis, Mo.: C. V. Mosby Co. 1968.Google Scholar

Copyright information

© Springer-Verlag 1970

Authors and Affiliations

  • S. David Rockoff
    • 1
  • John D. ArmstrongJr.
    • 1
  1. 1.Department of RadiologyWashington University School of MedicineSt. Louis

Personalised recommendations