Abstract
Removal of the soluble proteoglycans from slices of bovine costal cartilage by extraction in 4 M guanidinium hydrochloride permitted the visualization of abundant amounts of dispersed and disaggregated collagen in the matrix. Proteoglycans which are resistant to extraction are seen as small granules which are concentrated in the perilacunar regions. Large proteoglycan granules appear to originate in the chondrocyte. As they come to occupy positions in the matrix distant from the chondrocyte, the granules become smaller. A non-granular, “amorphous” component masks the collagen fibers so that they cannot be readily seen in the intact cartilage.
Résumé
L'élimination de protéoglycans solubles de coupes de cartilage costal de boeuf, par extraction dans une solution de 4M d'hydrochlorure de guanidinium, permet de mettre en évidence des quantités abondantes de collagène dispersé et désagrégé dans la matrice. Les protéoglycanes, résistants à l'extraction, sont visibles sous forme de granules concentrés dans les régions périlacunaires. Les granulations plus importants des protéoglycanes semblent venir du chondrocyte. Dans la matrice, éloignée des chondrocytes, ces granules deviennent plus étroites. Un composant non granulaire “amorphe” masque les fibres de collagène, de telle sorte qu'elles sont difficilement visibles dans le cartilage intact.
Zusammenfassung
Die löslichen Proteoglycane wurden mittels Extraktion in 4 M Guanidinhydrochlorid aus Rippenknorpelschnitten des Rindes entfernt. Dies erlaubte die Sichtbarmachung von großen Mengen von verstreuten und auseinandergerissenen Collagen in der Matrix. Die Protoglycane, welche sich nicht extrahieren lassen, erscheinen als kleine, in den perilacunären Regionen konzentrierte Körnchen. Die großen Proteoglycan-Körner scheinen in den Chondrocyten zu entstehen. Sobald sie sich in die Matrix, außerhalb der Chondrocyten, verlagern, werden die Körner kleiner. Ein nicht-granulärer, „amorpher” Bestandteil verhüllt die Collagenfasern, so daß diese im intakten Knorpel nicht deutlich gesehen werden können.
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References
Anderson, H. C., Sajdera, S. W.: The fine structure of bovine nasal cartilage. Extraction as a technique to study proteoglycans and collagen in cartilage matrix. J. Cell Biol.49, 650–663 (1971).
Cameron, D. A.: The fine structure of bone and calcified cartilage. A critical review of the contribution of electron microscopy to the understanding of osteogenesis. Clin. Orthop.26, 199–228 (1963).
Campo, R. D., Bielen, R. J., Hetherington, J.: Metabolic studies on the protein-polysaccharides of cartilage. Biochim. biophys Acta (Amst.)261, 136–142 (1972).
Campo, R. D., Tourtellotte, C. D.: The composition of bovine cartilage and bone. Biochim. biophys Acta (Amst.)141, 614–624 (1967).
Campo, R. D., Tourtellotte, C. D., Bielen, R. J.: The proteinpolysaccharides of articular, epiphyseal plate and costal cartilages. Biochim. biophys Acta (Amst.)177, 501–511 (1969).
Dische, Z.: A new specific color reaction of hexuronic acids. J. biol. Chem.167, 189–198 (1947).
Eisenstein, R., Sorgente, N., Kuettner, K. E.: Organization of extracellular matrix in epiphyseal growth plate. Amer. J. Path.65, 515–528 (1971).
Godman, G. C., Porter, K. R.: Chondrogenesis, studied with the electron microscope. J. biophys. biochem. Cytol.8, 719–760 (1960).
Geol, S. C.: Electron microscopic studies on developing cartilage. J. Embryol. exp. Morph.23, 169–184 (1970).
Khan, T. A., Overton, J.: Lanthanum staining of developing chick cartilage and reaggregating cartilage cells. J. Cell Biol.44, 433–438 (1970).
Knese, K. H.: Cytologische Beobachtungen an Skeletzellen über die Bildung der Kohlenhydrat-protein-komplexe. Z. mikr.-anat. Forsch.81, 233–294 (1969).
Kuettner, K. E.: Discussant in the comparative Molecular biology of extracellular matrices, ed. by H. C. Slavkin, p. 36–37. New York and London: Academic Press 1972).
Malawista, I., Schubert, M.: Chondromucoprotein: new extraction method and alkaline degradation. J. biol. Chem.230, 535–544 (1958).
Mathews, M. B.: The interactionof collagen and acid mucopolysaccharides. Biochem. J.96, 710–716 (1965).
Matukas, V. J., Panner, B. J., Orbison, J. L.: Studies on the ultrastructure, identification and distribution of protein-polysaccharides in cartilage matrix. J. Cell. Biol.32, 365–378 (1967).
Pal, S., Schubert, M.: The action of hydroxylamine on the proteinpolysaccharides of cartilage. J. biol. Chem.240, 3245–3248 (1965).
Reed, R., Wood, M. J., Keech, M. K.: Helical nature of the collagen fibril. Nature (L9nd,)177, 697–699 (1956).
Rosenberg, L., Johnson, B., Schubert, M.: The proteinpolysaccharides of human costal cartilage. J. clin. Invest.48, 543–552 (1969).
Sajdera, S. W., Hascall, V. C.: Proteinpolysaccharide complex from bovine nasal cartilage. A comparison of low and high shear extractive procedures. J. biol. Chem.244, 77–87 (1969).
Szirmai, J. A.: Structure of cartilage, in aging of Connective and skeletal tissue, ed. By A. Engel and T. Larsson, p. 163–184. Nordiska Bokhandelns Förlag Stockholm: 1969.
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Campo, R.D., Phillips, S.J. Electron microscopic visualization of proteoglycans and collagen in bovine costal cartilage. Calc. Tis Res. 13, 83–92 (1973). https://doi.org/10.1007/BF02015399
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DOI: https://doi.org/10.1007/BF02015399