Calcified Tissue Research

, Volume 13, Issue 1, pp 27–40 | Cite as

Influence of pyrophosphate on the exchange of calcium and phosphate ions on hydroxyapatite

  • A. Jung
  • S. Bisaz
  • P. Bartholdi
  • H. Fleisch
Original Papers

Abstract

The effect of pyrophosphate on the distribution and the rates of exchange of calcium and phosphate in hydroxyapatite crystals was studied. Analysis of disappearance of45Ca,32P [Pi] and32P [PPi] from the solution onto the crystals was performed using a compartmental model. The disappearance curves were described by the sum of 4 exponentials, which was interpreted as a four-compartment model in series. Pyrophosphate was present in all the four pools of the crystals. Treatment of crystal with pyrophosphate led to a large decrease of exchangeable phosphate, and two of the four compartments disappeared completely. The quantitative data suggest a replacement of 2 moles of orthophosphate by 1 mole of pyrophosphate. Pyrophosphate had no appreciable effect on the distribution and the fluxes of calcium. The implications of these results for the inhibitory effects of pyrophosphate on the formation and dissolution of apatite crystals are discussed.

Key words

Pyrophosphate Calcium Phosphate Kinetics Hydroxyapatite 

Résumé

L'effet du pyrophosphate sur la distribution et les vitesses d'échange du calcium et du phosphate dans les cristaux d'hydroxyapatite a été étudié. Les analyses de disparition du45Ca, du32P [Pi] et du32P [PPi] de la solution vers les cristaux ont été réalisées en utilisant un modèle de compartimenst. Les courbes de disparition sont formées par la somme de 4 exponentielles, correspondant à un modèle de 4 compartiments en séries. Le pyrophosphate est présent dans les 4 compartiments du cristal. Le traitement du cristal avec le pyrophosphate provoque une diminution nette du phosphate échangeable et deux des quatre compartiments disparaissent totalement. Les données quantitatives suggèrent une substitution de 2 moles d'orthophosphate par mole de pyrophosphate. Le pyrophosphate n'a pas d'effet net sur la distribution et les échanges de calcium. Les conséquences de ces résultats sur les effets d'inhibition du pyrophosphate sur la formation et la dissolution des cristaux d'apatite sont discutées.

Zusammenfassung

Die Wirkung von Pyrophosphat auf die Verteilung und die Austauschrate von Calcium und Phosphat in Hydroxyapatitkristallen wurde untersucht. Das Verschwinden von45Ca,32P [Pi] und32P [PPi] aus der Lösung und die Anlagerung dieser Ionen auf den Kristallen wurde anhand eines Kompartiment-Modelles studiert. Die Verschwinde-Kurven wurden durch die Summe von 4 Exponentiellen beschrieben und mittels einem Modell von 4 Kompartimenten in Serie interpretiert. Wurde der Lösung Pyrophosphat zugesetzt, so war es in allen 4 Kompartimenten vorhanden. Ferner entstand eine starke Verminderung des austauschbaren Phosphates, wobei 2 der 4 Kompartimente vollständig verschwanden und 2 Mode Orthophosphat mit 1 Mol Pyrophosphat ausgetauscht wurden. Pyrosphosphat hatte keine nennenswerte Wirkung auf die Verteilung und die Flüsse des Calciums. Diese Befunde werden im Zusammenhang mit der Hemmwirkung des Pyrophosphates bei der Bildung und Auflösung von Apatitkristallen besprochen.

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Copyright information

© Springer-Verlag 1973

Authors and Affiliations

  • A. Jung
    • 3
    • 1
    • 2
  • S. Bisaz
    • 3
    • 1
    • 2
  • P. Bartholdi
    • 3
    • 1
    • 2
  • H. Fleisch
    • 3
    • 1
    • 2
  1. 1.ObservatoryUniversity of GenuaGenua
  2. 2.Laboratory for Experimental SurgeryDavos
  3. 3.Pathophysiological InstituteUniversity of BerneBerneSwitzerland

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