Microbial Ecology

, Volume 4, Issue 3, pp 249–261 | Cite as

Methanogenic fermentation of benzoate in an enrichment culture

  • Eylon R. Shlomi
  • A. Lankhorst
  • R. A. Prins


Enrichment cultures inoculated with black mud fermented benzoate according to the stoichiometric equation: 4 C6H5CO2H+18 H2O → 15 CH4+13 CO2.Trans-2-hydroxycyclohexanecarboxylate, 2-oxo-cyclohexanecarboxylate, pimelate, caproate, butyrate, acetate, and molecular hydrogen were shown to be regular components of the culture fluid occurring in low concentrations. Inhibition of methanogenesis by chloroform, 4-chlorobutyrate, or 2-bromooctanoate resulted in a cessation of the benzoate breakdown after all intermediates had accumulated. It is proposed that benzoate is fermented via a direct reductive pathway to butyrate, acetate, H2, and CO2, whereafter butyrate is converted to acetate and H2, and the latter substrates are fermented to CH4 and CO2 by methane producers.


Methane Fermentation Chloroform Butyrate Methane Producer 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Springer-Verlag New York Inc 1978

Authors and Affiliations

  • Eylon R. Shlomi
    • 1
  • A. Lankhorst
    • 1
  • R. A. Prins
    • 1
  1. 1.Laboratory of Veterinary BiochemistryState University of UtrechtUtrechtThe Netherlands

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