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Pyridinium 2-azo-p-dimethylaniline chromophore, a chromogenic reagent for beta-lactamase testing compared to nitrocefin

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Abstract

Pyridinium-2-azo-p-dimethylaniline chromophore was evaluated as a test tube, filter paper and spectrophotometric assay for detection ofβ-lactamases from grampositive and gram-negative organisms. Although useful for detection of TEMβ-lactamases inHaemophilus influenzae andNeisseria gonorrhoeae, it was a poor agent for detecting TEM, OXA and PSE enzymes inEnterobacteriaceae. It also proved poor for detecting cephalosporinases inPseudomonas aeruginosa andEnterobacteriaceae, and penicillinases inStaphylococcus aureus when compared to nitrocefin. As a spectrophotometric substrate it was equivalent to nitrocefin and cephaloridine with variousβ-lactamases.

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Authors and Affiliations

  1. Division of Infectious Diseases, Department of Medicine, College of Physicians & Surgeons, Columbia University, 630 West 168 Street, 10032, New York, New York, USA

    T. Barlam & H. C. Neu

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  1. T. Barlam
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  2. H. C. Neu
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Barlam, T., Neu, H.C. Pyridinium 2-azo-p-dimethylaniline chromophore, a chromogenic reagent for beta-lactamase testing compared to nitrocefin. Eur. J, Clin. Microbiol. 3, 185–189 (1984). https://doi.org/10.1007/BF02014875

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  • DOI: https://doi.org/10.1007/BF02014875

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