Summary
Approximately 600 different samples of the broad bean collection have been evaluated for crude protein content of the seeds.
111 samples were also evaluated for S-aminoacids. The values ranged from 19.5 to 34.0 for protein content and 0.97 to 1.45 for half-cystine and 0.62 to 0.99 for methionine. Protein content was negatively correlated with seed weights, highest values been found in the small seeded samples of Ethiopia and Afghanistan. Mediterranean samples exibited quite large variability. S-aminoacid per protein unit were negatively correlated with the protein content and determination coefficients had intermediate values. Sulphur aminoacids expressed as % of dry seed weight were positively correlated with protein content, but determination values were rather low. Analyses of protein and sulphur aminoacids in seeds from a 10×10 diallel cross scheme gave information on genetic mechanisms involved in the character's control; additive and non additive components of variance were significant, indicating difficulties in practical breeding programmes.
Zusammenfassung
Fast 600 Sortimentsnummern der Ackerbohnenkollektion wurden auf den Rohproteingehalt der Samen untersucht, 111 auch auf den auf Gehalt an schwefelhaltigen Aminosäuren. Der Rohproteingehalt schwankt von 19,5 bis 34,0%, der Gehalt an Cystein von 0,97 bis 1,45% und der an Methionin von 0,62 bis 0,99%. Der Rohproteingehalt war negativ mit dem Samengewicht korreliert, die höchsten Werte wurden bei den kleinsamigen Sippen aus Äthiopien und Afghanistan gefunden. Die Sippen aus dem Mittelmeergebiet zeigen eine sehr große Variabilität. Der relative Gehalt an S-haltigen Aminosäuren war negativ mit dem Rohproteingehalt korreliert und das Bestimmtheitsmaß hatte mittlere Werte. Der absolute Gehalt an S-haltigen Aminosäuren war positiv mit dem Rohproteingehalt korreliert, aber das Bestimmtheitsmaß war niedriger.
Die Analysen des Rohproteingehaltes sowie des Gehaltes an schwefelhaltigen Aminosäuren der Samen (von F1-Pflanzen) aus einem 10×10 Diallel-Kreuzungsschema ergaben Informationen über genetische Mechanismen in der Kontrolle der Merkmale; die additive und die nicht additive Varianzkomponente waren signifikant und offenbaren die Schwierigkeiten in den praktischen Züchtungsprogrammen.
Краткое содержание
Около 600 образцов колле кции конских бобов исследовалось на сод ержание сырого протеина в сем енах. 111 образцов анализиров ались и на содержание аминокислот, содержа щих серу (С-аминокислоты). С одержание сырого про теина колебалось от 19,5 до 34,0%; содержание цистеин а — от 0,97 до 1,45%, а метионина — от 0,62 до 0,99%. Содержание протеин а показало отрицател ьную корреляцию с весом семян; самое высокое с одержание было отмеч ено у мелкосемянных форм и з Эфиопии и Афганистан а. Средиземноморские формы оказались очень изме нчивыми. Относительное содер жание С-аминокислот п оказало отрицательную корреляцию с содержа нием сырого протеина, хотя коэффициент детерми нации был невысоким. Однако абсолютное содержан ие С-аминокислот имело п озитивную корреляцию с содержа нием сырого протеина, но коэффициент детерми нации был ниже.
Анализы содержания с ырого протеина и соде ржания С-аминокислот в семенах растений f1 (по схеме диаллельных ск рещиваний 10× 10) дали информацию о генетических механ измах, контролирующи х эти признаки; аддитивная и неаддитивная компо ненты вариансы были статистически досто верны, что говорит о сложнос ти проведения селекц ионных программ.
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Lafiandra, D., Polignano, G.B., Filippetti, A. et al. Genetic variability for protein content and S-aminoacids in broad-beans (Vicia faba L.). Die Kulturpflanze 29, 115–127 (1981). https://doi.org/10.1007/BF02014742
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DOI: https://doi.org/10.1007/BF02014742