Fasting or dexamethasone treatment reduce protease content in rat lung mast cells and modulation of histamine synthesis by H3 receptors
The sensitivity of mast cells to H3-receptor modulation was studied in rat lung under various hormonal conditions. The heterogeneity of mast cell subpopulations in rat lung was assessed by the tissue content of rat mast cell protease I (RMCP I) and rat mast cell protease II (RMCP II). After 24 h fasting, concentrations of RMCP I were unchanged whereas the concentration of RMCP II was significantly reduced by 49%. The [3H]histamine (HA) synthesis was concomitantly decreased by 35%. In addition, the modulation of [3H]HA, synthesis by the H3 receptor agonist, (R)α-methylHA and by the antagonist, thioperamide, observed in control rats, was lost in fasted rats. Single and repeated, administrations of dexamethasone did not influence RMCPI concentrations, but decreased the concentrations of RMCP II with a parallel decrease in [3H]HA synthesis. The inhibitory effect of (R)α-methylHA on [3H]HA synthesis was also reduced.
These results suggest that a subpopulation of RMCP II-containing mast cells, very sensitive to environmental factors, could be the mast cells synthesizing HA in an H3-receptor-dependant manner.
Key wordsRat mast cell proteases Histamine presynaptic receptor Dexamethasone
Unable to display preview. Download preview PDF.
- P. J. Barnes,Histamine receptors in the respiratory tract. In:The Histamine Receptor. Vol. 16 (Eds. J.-C. Schwartz and H. L. Haas) pp. 253–270. Wiley Liss Inc, New York, 1992.Google Scholar
- G. Bertaccini and G. Coruzzi,Histamine receptors in the digestive system. In:The Histamine Receptor. Vol. 16 (Eds. J.-C. Schwartz and H.L. Haas) pp. 193–230, Wiley Liss Inc., New York 1992.Google Scholar
- J.-C. Schwartz, J. M. Arrang, M. Garbarg, J. Moreau and H. Pollard,A third histamine receptor subtype: Pharmacology and functions. In:Mast Cell and Basophil Differentiation, and Function in Health and Disease. (Eds. S. J. Galli and K. F. Austen) pp. 285–294, Raven Press Ltd., New York 1989.Google Scholar
- L. Enerback,Mast cell heterogeneity: The evolution of the concept of a specific mucosal mast cell. In:Mast cell Differentiation and Heterogeneity. (Eds. A. D. Befus, J. Bienenstock and J. A. Denburg). pp. 1–26, Raven Press, New York (1986).Google Scholar
- H. R. P. Miller, J. F. Huntley, G. F. J. Newlands and J. Irvine,Granule chymases and the characterization of mast cell phenotype and function in rat and mouse. In:Neutral Proteases of Mast Cells. Monographs in Allergy. Vol. 27. (Ed. L. B. Schwartz). pp. 1–30, Karger, Basel 1990.Google Scholar
- J. F. Huntley, A. Mackellar, G. F. J. Newlands, J. Irvine and H. R. P. Miller,Mapping of the rat mast cell granule proteinases RMCP I and II by enzyme linked immunosorbant assay and paired immunofluorescence. APMIS28, 933–944 (1990).Google Scholar
- W. M. Hunter and F. C. Greenwood,Preparation of iodine-131 labelled human growth hormone of high specific activity. Nature194, 494–496 (1962).Google Scholar
- M. Garbarg, H. Pollard, T. T. Quach and J.-C. Schwartz,Methods in brain histamine research. In:Methods in Biogenic Amines Research (Eds S. Parvez, T. Nagatsu, I. Nagatsu and H. Parvez) pp. 623–662, Elsevier, Amsterdam 1983.Google Scholar
- R. P. Schleimer,The effects of anti-inflammatory steroids on mast cells. In:The Mast Cell in Health and Disease, Vol. 62. (Eds. M. A. Kaliner and D. D. Metcalfe). pp. 483–511, Marcel Dekker, Basel 1993.Google Scholar
- K. Soda, S. Kawabori, N. Kanai, J. Bienenstock and M. H. Perdue,Steroid-induced depletion of mucosal mast cells and eosinophils in intestine of athymic nude rats. Int. Arch. Allerg. Immunol.101, 39–46 (1993).Google Scholar
- H. Onoue, K. Maeyama, S. Nomura, T. Kasugai, H. Tei, H. M. Kim, T. Watanabe and Y. Kitamura,Absence of immature mast cells in the skin of Ws/Ws rats with a small deletion tyrosine kinase domain of the c-kit gene. Am. J. Physiol.142 1001–1007 (1993).Google Scholar
- T. Kasugai, M. Odaka, M. Morimoto, N. Arizono, K. Maeyama, M. Yamada, H. Tei, K. Dohmae, H. Onoue, G. F. J. Newlands, T. Watanabe, Y. Nishimune, H. R. P. Miller and Y. Kitamura,Infection of Nippostrongylus brasiliensis, induces normal increase of basophils in mast cell deficient Ws/Ws rats with a small deletion at the kinase domain of c-kit. Blood81, 2521–2529 (1993).PubMedGoogle Scholar
- I. N. M. Day,The diffuse neuroendocrine system: A molecular perspective. Mol. and Cell. Probes1, 275–295 (1987).Google Scholar
- V. Dimitriadou, A. Rouleau, M. Dam Trung Tuong, G. J. F. Newlands, H. R. P. Miller, G. Luffau, J.-C. Schwartz and M. Garbarg. Clinical Science, in press.Google Scholar