Abstract
An improved cell culture method for the recovery of Chlamydia trachomatis from clinical samples was evaluated. Freshly trypsinized HeLa 229 cells were infected in suspension culture and compared to a standard monolayer culture method. Among 1085 specimens evaluated, 84 were positive by both methods, 978 were negative by both methods, 2 were positive by the monolayer method only, and 21 were positive by the suspension method only. Inclusion counts were two-fold higher in the suspension culture method than in the monolayer method (p<.001). It is concluded that freshly trypsinized HeLa 229 cells infected in suspension culture are more susceptible to infection with Chlamydia trachomatis than standard monolayer cells.
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Oyelese, A.O., Brunham, R.C., McDowell, J. et al. Enhanced susceptibility of trypsinized HeLa cells toChlamydia trachomatis infection. Eur. J, Clin. Microbiol. 6, 594–596 (1987). https://doi.org/10.1007/BF02014259
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DOI: https://doi.org/10.1007/BF02014259