European Journal of Clinical Microbiology

, Volume 1, Issue 2, pp 97–101 | Cite as

Effect of subinhibitory antibiotic concentrations on the phagocytosis ofStaphylococcus aureus

  • D. Milatović
Article

Abstract

The effect of subinhibitory antibiotic concentrations on the phagocytosis ofStaphylococcus aureus was studied by pretreating3H-thymidine labelled bacteria with one-third the minimal inhibitory concentration of clindamycin, doxycyclin, cefotiam, vancomycin, piperacillin and penicillin G, respectively. Pretreatment with clindamycin and doxycyclin resulted in enhanced uptake of the bacteria by polymorphonuclear leukocytes compared to the untreated control. The augmented phagocytosis was still observed at 1/32 the MIC of clindamycin and 1/64 the MIC of doxycyclin, and when the serum was diluted to a concentration of 1 %. Pretreatment of the bacteria with penicillin, cefotiam, piperacillin and vancomycin had no effect on phagocytosis. Inhibitors of bacterial protein synthesis induce alterations ofStaphylococcus aureus leading to increased phagocytosis, whereas antibiotics acting on cell wall synthesis are without effect.

Keywords

Cell Wall Penicillin Protein Synthesis Minimal Inhibitory Concentration Vancomycin 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Lorian, V.: Some effects of subinhibitory concentrations of antibiotics on bacteria. Bulletin of the New York Academy of Medicine 1975, 51: 1046–1053.PubMedGoogle Scholar
  2. 2.
    Alexander, J. W., Good, R. A.: Effect of antibiotics on the bactericidal activity of human leukocytes. Journal of Laboratory and Clinical Medicine 1968, 71: 971–983.PubMedGoogle Scholar
  3. 3.
    Hoeprich, P. D., Martin, C. H.: Effect of tetracycline, polymyxin B and rifampin on phagocytosis. Clinical Pharmacology and Therapeutics 1970, 11: 418–422.PubMedGoogle Scholar
  4. 4.
    Pruul, H., McDonald, P. J.: Enhancement of antibacterial activity of human leukocytes againstEscherichia coli pretreated with chloramphenicol. In: Nelson, J. D., Grassi, C. (ed.): Current chemotherapy and infectious disease, Volume II. American Society for Microbiology, Washington D.C., 1980, p. 796–797.Google Scholar
  5. 5.
    Welch, W. D., Davis, D., Thrupp, L. D.: Effect of antimicrobial agents on human polymorphonuclear leukocyte microbicidal function. Antimicrobial Agents and Chemotherapy 1981, 20: 15–20.PubMedGoogle Scholar
  6. 6.
    Forsgren, A., Gnarpe, H.: Tetracyclines and hostdefense mechanism. Antimicrobial Agents and Chemotherapy 1973, 3: 711–715.PubMedGoogle Scholar
  7. 7.
    Forsgren, A., Schmeling, D., Quie, P. G.: Effect of tetracycline on the phagocytic function of human leukocytes. Journal of Infectious Diseases 1974, 130: 412–415.PubMedGoogle Scholar
  8. 8.
    Melby, K., Midtvedt, T.: Effects of some antibacterial agents on the phagocytosis of32P-labelledEscherichia coli by human polymorphonuclear cells. Acta Pathologica et Microbiologica Scandinavica (B) 1980, 88: 103–106.Google Scholar
  9. 9.
    Gemmell, C. G., Abdul-Amir, M. K.: Antibiotic-induced changes in streptococci with respect to their interaction with human polymorphonuclear leukocytes. In: Nelson, J. D., Grassi, C. (ed.): Current chemotherapy and infectious disease, Volume II. American Society for Microbiology, Washington D.C., 1980, p. 810–812.Google Scholar
  10. 10.
    Root, R. K., Isturiz, R., Molavi, A., Metcalf, J. A., Malech, H. L.: Interactions between antibiotics and human neutrophils in the killing of staphylococci. Journal of Clinical Investigation 1981, 67: 247–259.PubMedGoogle Scholar
  11. 11.
    Friedman, H., Warren, G. H.: Enhanced susceptibility of penicillin-resistant staphylococci to phagocytosis after in vitro incubation with low doses of nafcillin. Proceedings of the Society for Experimental Biology and Medicine 1974, 146: 707–711.PubMedGoogle Scholar
  12. 12.
    Nishida, M., Mine, Y., Nonoyama, S., Yokota, Y.: Effect of antibiotics on the phagocytosis and killing ofPseudomonas aeruginosa by rabbit polymorphonuclear leukocytes. Chemotherapy 1976, 22: 203–210.PubMedCrossRefGoogle Scholar
  13. 13.
    Braveny, I., Dickert, H., Machka, K.: Antibakterielle Aktivität von Cefotaxim im Vergleich mit sieben Cephalosporinen. Infection 1979, 7: 231–236.CrossRefPubMedGoogle Scholar
  14. 14.
    Verhoef, J., Peterson, P. K., Quie, P. G.: Kinetics of staphylococcal opsonization, attachment, ingestion and killing by human polymorphonuclear leukocytes: a quantitative assay using3H-thymidine labelled bacteria. Journal of Immunological Methods 1977, 14: 303–311.CrossRefPubMedGoogle Scholar
  15. 15.
    Lorian, V.: Effects of subminimum inhibitory concentrations of antibiotics on bacteria. In: Lorian, V. (ed.): Antibiotics in laboratory medicine. Williams and Wilkins, Baltimore, 1980, p. 342–408.Google Scholar
  16. 16.
    Gemmell, C. G., Peterson, P. K., Schmeling, D., Kim, Y., Mathews, J., Wannamaker, L., Quie, P. G.: Potentiation of opsonisation and phagocytosis ofStreptococcus pyogenes following growth in the presence of clindamycin. Journal of Clinical Investigation 1981, 67: 1249–1256.PubMedCrossRefGoogle Scholar

Copyright information

© Vieweg Publishing 1982

Authors and Affiliations

  • D. Milatović
    • 1
  1. 1.Institute of Medical MicrobiologyTechnical University MunichMunich 80FRG

Personalised recommendations