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Antibody-dependent cell-mediated cytotoxicity againstEscherichia coli O antigens

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Abstract

Antibodies againstEscherichia coli O antigen from rabbits immunized with formalin-killed bacteria were tested for cytotoxic capacity in an antibody-dependent cell-mediated cytotoxicity (ADCC) assay with human lymphocytes as effector cells and autologous papainized erythrocytes coated with O antigen as target cells. The cytotoxic titres were compared with the titres obtained with three methods of antibody quantitation. It was found that ADCC recorded antibodies with similar sensitivity as the enzyme-linked immunosorbent assay (ELISA) for IgG, but was much more sensitive than the ammonium sulphate precipitation (ASP) and indirect haemagglutination (IHA) usingβ-mercaptoethanol reduced sera. The ADCC titres were found to correlate very well with the titres obtained with ASP, ELISA and IHA for IgG but not for IgM, which is in accordance with a previous notion that ADCC is primarily mediated via IgG antibodies. ADCC should be considered as a possible immunopathologic mechanism in renal parenchymal damage in connection with urinary tract infections.

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Authors and Affiliations

  1. Department of Clinical Immunology, Institute of Medical Microbiology, University of Göteborg, Guldhedsgatan 10 A, S-413 46, Göteborg, Sweden

    M. Hagberg & L. Hanson

  2. Department of Pediatrics, University of Göteborg, Guldhedsgatan 10 A, S-413 46, Göteborg, Sweden

    M. Hagberg & L. Hanson

  3. Pharmacia Diagnostics AB, Box 17, S-751 03, Uppsala, Sweden

    S. Ahlstedt

Authors
  1. M. Hagberg
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  2. S. Ahlstedt
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  3. L. Hanson
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Hagberg, M., Ahlstedt, S. & Hanson, L. Antibody-dependent cell-mediated cytotoxicity againstEscherichia coli O antigens. Eur. J, Clin. Microbiol. 1, 59–65 (1982). https://doi.org/10.1007/BF02014142

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