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Serum-induced lysis ofPseudomonas aeruginosa

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Abstract

The sensitivity of 12Pseudomonas aeruginosa strains (5 mucoid and 7 non-mucoid strains) to serum and the interaction of these strains with the complement system was studied. Five strains (4 mucoid and 1 non-mucoid strains) were lysed in 20 % normal serum as measured by the release of rediolabelled material from3H-adenine labelled bacteria. Three of these strains were also lysed in MgEGTA chelated serum. All strains activated complement via the classical pathway, and six strains were able to activate the alternative complement pathway as well. Slime production did not interfere with bacteriolysis and complement consumption.

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Authors and Affiliations

  1. Microbiology Laboratory, State University of Utrecht, Catharijnesingel 59, 3511, GG Utlecht, The Netherlands

    T. Meshulam, H. Verbrugh & J. Verhoef

  2. Department of Microbiology, Rambam Hospital Center, Bat Galim, Haifa, Israel

    T. Meshulam

Authors
  1. T. Meshulam
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  2. H. Verbrugh
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  3. J. Verhoef
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Additional information

This paper is part of a D.Sc. thesis to be submitted to the Senate of the Technion Israel Institute of Technology, Haifa, Israel.

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Meshulam, T., Verbrugh, H. & Verhoef, J. Serum-induced lysis ofPseudomonas aeruginosa . Eur. J, Clin. Microbiol. 1, 1–6 (1982). https://doi.org/10.1007/BF02014132

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