Abstract
Six monoclonal antibodies raised againstEscherichia coli O111 and against its rough mutant J5 (chemotype Rc) were studied. One IgG2A, one IgM anti-J5, and one IgG2A anti-O111 monoclonal antibody did not bind to lipopolysaccharides of the homologous strain, but cross-reacted with heterologous gram-negative rods in an enzyme-linked immunosorbent assay. These three monoclonal antibodies activated complement when incubated with homologous or heterologous strains, but were opsonic neither in the presence nor in the absence of complement. The other three monoclonal antibodies were directed against lipopolysaccharide of the homologous strain, but showed no cross-reactivity. The IgG3 and one IgM anti-J5 monoclonal antibodies activated complement and were opsonic only in the presence of complement. The IgM anti-O111 monoclonal antibody activated complement and was opsonic both in the presence and absence of complement. Thus, the outcome of the interaction between bacteria, antibodies, and complement is influenced primarily by whether antibodies are directed against lipopolysaccharides or against other cell wall components.
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Lüderitz, O., Staub, A. M., Westphal, O.: Immunochemistry of O and R antigens ofSalmonella and relatedEnterobacteriaceae. Bacteriological Reviews 1966, 30: 192–255.
Braude, A. I., Douglas, H.: Passive immunization against the local Schwartzman reaction. Journal of Immunology 1972, 108: 505–512.
Davis, C. E., Ziegler, E. J., Arnold, K. F.: Neutralization of meningococcal endotoxin by antibody to core glycolipid. Journal of Experimental Medicine 1978, 147: 1007–1017.
Johns, M., Skehill, A., McCabe, W. R.: Immunization with rough mutants ofSalmonella minnesota. IV. Protection by antisera to O and rough antigens against endotoxin. Journal of Infectious Diseases 1983, 147: 57–67.
Marks, M. I., Ziegler, E. J., Douglas, H., Corbeil, L. B., Braude, A. I.: Induction of immunity against lethalHaemophilus influenzae type b infection byEscherichia coli core lipopolysaccharide. Journal of Clinical Investigation 1982, 69: 742–749.
McCabe, W. R.: Immunization with R mutants ofSalmonella minnesota. I. Protection against challenge with heterologous gram-negative bacilli. Journal of Immunology 1972, 108: 601–610.
Young, L. S., Stevens, P., Ingram, J.: Functional role of antibody against “core” glycolipid ofEnterobacteriaceae. Journal of Clinical Investigation 1975, 56: 850–861.
Ziegler, E. J., Douglas, H., Sherman, J. E., Davis, C. E., Braude, A. I.: Treatment ofE. coli andKlebsiella bacteremia in agranulocytic animals with antiserum to a UDP-gal epimerase-deficient mutant. Journal of Immunology 1973, 111: 433–438.
Ziegler, E. J., McCutchan, J. A., Fierer, J., Glauser, M. P., Sadoff, J. C., Douglas, B. S., Braude, A. I.: Treatment of gram-negative bacteremia and shock with human antiserum to a mutantEscherichia coli. New England Journal of Medicine 1982, 307: 1225–1230.
Wolff, S. M.: The treatment of gram-negative bacteremia and shock. Editorial. New England Journal of Medicine 1982, 307: 1267–1268.
Kirkland, T. N., Ziegler, E. J.: An immunoprotective monoclonal antibody to lipopolysaccharide. Journal of Immunology 1984, 132: 2590–2592.
Nelles, M. J., Niswander, C. A.: Mouse monoclonal antibodies reactive with J5 lipopolysaccharide exhibit extensive serological cross-reactivity with a variety of gram-negative bacteria. Infection and Immunity 1984, 46: 677–681.
Ivanoff, B., André, C., Fontanges, R., Jourdan, G.: Secondary immune response to oral and nasal rough mutant strains ofSalmonella typhimurium. Annales d'Immunologie (L'Institut Pasteur) 1982, 133 D: 61–70.
Shulman, M.: A better cell line for making hybridomas secreting specific antibodies. Nature 1978, 276: 269–270.
Köhler, G., Milstein, C.: Continuous culture of fused cells secreting antibody of predefined specificity. Nature 1975, 256: 495–497.
Fazekas de St. Groth, S., Scheidegger, D.: Production of monoclonal antibodies: strategy and tactics. Journal of Immunological Methods 1980, 35: 1–21.
Westphal, O., Jann, K.: Bacterial lipopolysaccharides. Methods of Carbohydrate Research 1965, 5: 83–91.
Galanos, C., Lüderitz, O., Westphal, O.: A new method for the extraction of R lipopolysaccharides. European Journal of Biochemistry 1969, 9: 245–249.
Bos, E. S., van der Doelen, A. A., van Rooy, N., Schuurs, A. H. W. M.: 3,3′,5,5′-Tetramethylbenzidine as an ames test negative chromogen for horse-radish-peroxidase in enzyme-immunoassay. Journal of Immunoassay 1981, 2: 187–204.
Klerx, J. P. A. M., Beukelman, C. J., van Dijk, H., Willers, J. M. N.: Microassay for colorimetric estimation of complement activity in guinea pig, human and mouse serum. Journal of Immunological Methods 1983, 63: 215–220.
Böyum, A.: Isolation of mononuclear cells and granulocytes from human blood. Scandinavian Journal of Clinical Investigation 1968, 97: S77–89.
Verbrugh, H. A., Peters, R., Peterson, P. K., Verhoef, I.: Phagocytosis and killing of staphylococci by human polymorphonuclear and mononuclear leukocytes. Journal of Clinical Pathology 1978, 31: 539–545.
Verhoef, J., Peterson, P. K., Quie, P. G.: Kinetics of staphylococcal opsonization, attachment, ingestion and killing by human polymorphonuclear leukocytes: a quantitative assay using3H-thymidine labeled bacteria. Journal of Immunological Methods 1977, 14: 303–311.
Rozenberg-Arska, M., Salters, M. E. C., van Strijp, J. A. G., Geuze, J. J., Verhoef, J.: Electron microscopic study of phagocytosis ofEscherichia coli by human polymorphonuclear leukocytes. Infection and Immunity 1985, 50: 852–859.
Couderc, J., Kazatchkine, M. D., Ventura, M., Thien Duc, H., Maillet, F., Thobie, N., Liacopoulos, P.: Activation of the human classical complement pathway by a mouse monoclonal hybrid IgG1-2A monovalent anti-TNP antibody bound to TNP-conjugated cells. Journal of Immunology 1985, 134: 486–491.
Betz, I., Isliker, H.: Antibody-independent interactions betweenEscherichia coli J5 and human complement components. Journal of Immunology 1981, 127: 1748–1754.
Tenner, A. J., Ziccardi, R. J., Cooper, N. R.: Antibody-independent Cl activation byE. coli. Journal of Immunology 1984, 133: 886–891.
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Vreede, R.W., Leuvenink, J., Bouter, A.S. et al. Complement activating and opsonic capacity of monoclonal antibodies raised againstEscherichia coli O111 and its rough mutant J5. Eur. J, Clin. Microbiol. 5, 141–147 (1986). https://doi.org/10.1007/BF02013969
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DOI: https://doi.org/10.1007/BF02013969