Enzyme-linked immunosorbent assay for detection of immunoglobulin G and M antibodies to teichoic acid in intravascular staphylococcal disease
An enzyme-linked immunosorbent assay (ELISA) for detection of IgG and IgM antibodies to cell-wall teichoic acids ofStaphylococcus aureus and three defined coagulase-negative staphylococci was tested using serum samples from 11 cases of intravascular coagulasenegative staphylococcal infections, 13 cases ofStaphylococcus aureus endocarditis, and 24 patients with no evidence of infection. IgG antibody titers to all four teichoic acids in the 13 patients withStaphylococcus aureus endocarditis were significantly different from those in noninfected control patients (p<0.0001). In contrast, IgG antibody titers in serum from 11 cases of intravascular coagulase-negative Staphylococcal infection were not significantly different from those in control sera. There were no differences in IgM antibody titers of the three groups. Although the ELISA was sensitive in detectingStaphylococcus aureus endocarditis, it was not reliable in the detection of intravascular coagulasenegative Staphylococcal infections, even when tested with specific teichoic acid.
KeywordsInternal Medicine Serum Sample Antibody Titer Endocarditis Control Patient
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- 3.Tuazon, C. U., Sheagren, J. N., Choa, M. S., Marcus, D., Austin, J. A.:Staphylococcus aureus bacteremia: relationship between teichoic acid antibody formation and the development of metastatic abscesses. Journal of Infectious Diseases 1978, 131: 57–62.Google Scholar
- 4.Oeding, P.: GenusStaphylococcus. In: Bergen, J., Norris, J. (ed.): Methods in microbiology. Volume 12. Academic Press, New York, 1978, p. 127–176.Google Scholar
- 6.Engvall, E., Perlman, P.: Enzyme-linked immunosorbent assay, ELISA. III. Quantitation of specific antibodies by enzyme-labelled anti-immunoglobulin in antigen-coated tubes. Journal of Immunology 1972, 109: 129–135.Google Scholar
- 10.Bayston, R.: Serological indication ofStaphylococcus albus infection in children with colonized shunts. Developmental Medicine and Child Neurology 1971, 25, Supplement 13: 135–136.Google Scholar
- 12.Bayston, R.: Serological surveillance of children with CSF shunting devices. Developmental Medicine and Child Neurology 1975, 35, Supplement 17: 104–110.Google Scholar
- 13.Inman, R. D., Gallegos, K. V., Brause, B. D., Redecha, P. B., Christian, C. L.: Clinical and microbial features of prosthetic joint infection. American Journal of Medicine 1984, 77: 47–53.Google Scholar