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Early diagnosis of Q fever: Detection of immunoglobulin M by radioimmunoassay and enzyme immunoassay

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Abstract

The use of a radioimmunoassay and an enzyme immunoassay for early diagnosis of Q fever is described, both of which are based on the IgM antibody-capture principle. A commercially available phase II antigen and a labeled, purified anti-Q-IgG of human origin were employed. With these tests Q fever antibodies were detected earlier in the course of infection than with the complement fixation test.

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References

  1. Hunter, W. M., Greenwood, F. C.: Preparation of iodine-131 labelled human growth hormone of high specific activity. Nature 1962, 194: 495–496.

    PubMed  Google Scholar 

  2. Wilson, M. B., Nakane, P. K.: Recent developments in the peroxidase methods of conjugating horse radish peroxidase (HRPO) to antibodies. In: Knapp, W. (ed.): Immunofluorescence and related techniques. Elsevier, Amsterdam, 1978, p. 215–224.

    Google Scholar 

  3. Flehmig, B., Ranke, M., Berthold, H., Gerth, H. J.: A solid-phase radioimntunoassay for detection of IgM antibodies to hepatitis A virus. Journal of Infectious Diseases 1979, 140: 169–175.

    PubMed  Google Scholar 

  4. Lennette, E. H., Schmidt, N. J.: Diagnostic procedures for viral and rickettsial infections. American Public Health Association, New York, 1969, p. 52–58.

    Google Scholar 

  5. Döller, G., Flehmig, B., Schmitz, H.: A comparison of enzyme immunoassay and radioimmunoassay for the detection of hepatitis A virus and antibodies against hepatitis A virus. Journal of Biological Standardization 1984, 12: 47–59.

    PubMed  Google Scholar 

  6. Hunt, J. G., Field, P. R., Murphy, A. M.: Immunoglobulin responses toCoxiella burnetii (Q-Fever): single-serum diagnosis of acute infection, using an immunofluorescence technique. Infection and Immunity 1983, 39: 977–981.

    PubMed  Google Scholar 

  7. Pecock, M. G., Philip, R. N., Williams, J. C., Faulkner, R. S.: Serological evaluation of Q fever in humans: enhanced phase I titers of immunoglobulins G and A are diagnostic for Q-fevet endocarditis. Infection and Immunity 1983, 41: 1089–1089.

    PubMed  Google Scholar 

  8. Field, P. R., Hunt, J. G., Murphy, A. M.: Detection and persistence of specific IgM antibodies toCoxiella burnetii by enzyme-linked immunosorbent assay: a comparison with immunofluorescence and complement fixation tests. Journal of Infectious Diseases 1983, 148: 477–487.

    PubMed  Google Scholar 

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Authors and Affiliations

  1. Abteilung für Medizinische Virologie, Hygiene-Institut der Universität, Silcherstraße 7, 7400, Tübingen, FRG

    G. Döller, P. C. Döller & H. -J. Gerth

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  1. G. Döller
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  2. P. C. Döller
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  3. H. -J. Gerth
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Döller, G., Döller, P.C. & Gerth, H.J. Early diagnosis of Q fever: Detection of immunoglobulin M by radioimmunoassay and enzyme immunoassay. Eur. J, Clin. Microbiol. 3, 550–553 (1984). https://doi.org/10.1007/BF02013617

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  • DOI: https://doi.org/10.1007/BF02013617

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