European Journal of Clinical Microbiology

, Volume 3, Issue 6, pp 546–549 | Cite as

Niacin, nitrate and pyrazinamide studies using Middlebrook 7H10 Broth

  • J. J. Damato
  • M. T. Collins
  • J. K. McClatchy
Short Reports


An innovative and rapid method for testing mycobacteria was developed using Middlebrook 7H10 Tween Broth in place of conventional media. Niacin production, nitrate reduction and the breakdown of pyrazinamidase were determined in 198 mycobacteria isolates. Less than nine days were required to obtain positive test results, and the correlation of tween broth with conventional test methods exceeded 98 %.


Nitrate Internal Medicine Niacin Positive Test Rapid Method 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Vieweg Publishing 1984

Authors and Affiliations

  • J. J. Damato
    • 1
  • M. T. Collins
    • 2
  • J. K. McClatchy
    • 3
  1. 1.Department of PathologyFitzsimons Army Medical CenterAuroraUSA
  2. 2.Department of Pathobiological Sciences, School of Veterinary MedicineUniversity of WisconsinMadisonUSA
  3. 3.Clinical LaboratoriesNational Jewish Hospital and Research CenterDenverUSA

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