Abstract
Providencia stuartii has emerged as a significant nosocomial urinary tract pathogen. An increase in the number ofProvidencia isolates from urine cultures prompted an investigation into the possibility of an outbreak due to this organism. A high proportion of patients studied had urinary devices. Four wards were screened at two time periods to ascertain the prevalence ofProvidencia stuartii in urine cultures. Biotype, serotype, antibiogram and plasmid content were determined for eachProvidencia isolate. Of 129 patients initially sampled 22.5 % were found to harborProvidencia stuartii. Biotyping, serotyping and antibiograms indicated an epidemic strain was not present. Similar results were obtained when the wards were screened a second time, with 25.4% of urine cultures found to containProvidencia stuartii. By plasmid analysis the isolates could be grouped into one of ten profiles. A correlation could be made between urease activity and the presence of a large plasmid. No association however could be made between a particular plasmid profile and antibiogram. The data indicate that an epidemic strain ofProvidencia stuartii was not present. The source(s) of the endemicProvidencia stuartii strains remain unknown.
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References
Overturf, G. D., Wilkins, J., Ressler, R.: Emergence or resistance ofProvidencia stuartii to multiple antibiotics: speciation and biochemical characterization ofProvidencia. Journal of Infectious Disease 1974, 129: 353–357.
Keane, C. T., English, L. F., Wise, R.:Providencia stuartii infections. Lancet 1975, ii: 1045.
Klastersky, J., Bogaertes, A. M., Noterman, J., VanLaer, E., Daneau, D., Mouawad, E.: Infections caused by providence bacilli. Scandinavian Journal of Infectious Disease 1974, 6: 153–160.
Milstoc, M., Steinberg, P.: Fatal septicemia due to providence group bacilli. Journal of the American Geriatric Society 1973, 21: 159–163.
Solberg, C. O., Matsen, J. M.: Infections with providence bacilli. A clinical and bacteriologic study. American Journal of Medicine 1975, 50: 241–246.
Curreri, P. W., Bruck, H. M., Lindberg, R. B., Mason, A. D., Pruitt, B. A.:Providencia stuartii sepsis: a new challenge in the treatment of thermal injury. Annals of Surgery 1973, 177: 133–138.
Wenzel, R. P., Hunting, K. J., Osterman, C. A., Sande, M. A.:Providencia stuartii, a hospital pathogen: potential factors for its emergence and transmission. American Journal of Epidemiology 1976, 104: 170–180.
Whiteley, G. R., Penner, J. L., Stewart, I. O., Stokan, P. C., Hinton, N. A.: Nosocomial urinary tract infections caused by two O-serotypes ofProvidencia stuartii in one hospital. Journal of Clinical Microbiology 1977, 6: 551–554.
Fierer, J., Ekstrom, M.: An outbreak ofProvidencia stuartii urinary tract infections. Patients with condom catheters are a reservoir of the bacteria. Journal of the American Medical Association 1981, 17: 1553–1554.
Washington, J. A., Senjem, D. H., Haldorson, A., Schutt, A. H., Martin, W. J.: Nosocomially acquired bacteriuria due toProteus rettgeri andProvidencia stuartii. American Journal of Clinical Pathology 1973, 60: 836–838.
Kocka, F. E., Srinivasan, S., Mowjood, M., Kantor, H. S.: Nosocomial multiply resistantProvidencia stuartii: a long-term outbreak with multiple biotypes and serotypes at one hospital. Journal of Clinical Microbiology 1980, 11: 167–169.
Penner, J. C., Hinton, N. A., Hamilton, L. J., Hennessy, J. N.: Three episodes of nosocomial urinary tract infections caused by one O-serotype ofProvidencia stuartii. Journal of Urology 1981, 125: 668–671.
Hawkey, P. M., Penner, J. L., Potten, M. R., Stephens, M., Barton, L. J., Speller, D. C. E.: Prospective survey of fecal, urinary tract, and environmental colonization byProvidencia startii in two geriatric wards. Journal of Clinical Microbiology 1982, 16: 422–426.
Penner, J. L., Hinton, N. A., Whitely, G. R., Hennessy, J. N.: Variation in urease activity of endemic hospital strains ofProteus rettgeri andProvidencia stuartii. Journal of Infectious Disease 1976, 134: 370–376.
National Committee for Clinical Laboratory Standards. Tentative Standard M2–M3. Performance standards for antimicrobial disk susceptibility tests. National Committee for Clinical Laboratory Standards, Villanova, PA 1983.
Penner, J. L., Hinton, N. A., Hennessy, J. N., Whitely, G. R.: Reconstitution of the somatic (O-) antigenic scheme forProvidencia and preparation of O-typing antisera. Journal of Infectious Diseases 1976, 133: 283–292.
Kado, C. I., Liu, S. T.: Rapid procedure for detection and isolation of large and small plasmids. Journal of Bacteriology 1981, 145: 1365–1373.
Macrina, F. L., Kopecko, D. J., Jones, K. R., Ayers, D. J., McCawen, S. M.: A multiple plasmid containingEscherichia coli strain: convenient source of size reference plasmid molecules. Plasmid 1978, 1: 417–420.
Christensen, W. B.: Urea decomposition as a means of differentiatingProteus and paracolon cultures from each other and fromSalmonella andShigella types. Journal of Bacteriology 1946, 52: 461–466.
Standfast, S. J., Michelsen, P. B., Balten, A. L., Smith, R. P., Latham, E. K., Spellacy, A. B., Venezia, R. A., Andritz, M. H.: A prevalence survey of infections in a combined acute and long-term care hospital. Infection Control 1984, 5: 177–184.
McHale, P. J., Keane, C. T., Corigan, G.: Antibiotic resistance inProvidencia stuartii isolated in hospitals. Journal of Clinical Microbiology 1981, 13: 1099–1104.
Grant, R. B., Penner, J. L., Hennessy, J. N., Jackowski, B. J.: Transferable urease activity inProvidencia stuartii. Journal of Clinical Microbiology 1981, 13: 561–565.
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Hollick, G.E., Nolte, F.S., Calnan, B.J. et al. Characterization of endemicProvidencia stuartii isolates from patients with urinary devices. Eur. J, Clin. Microbiol. 3, 521–525 (1984). https://doi.org/10.1007/BF02013611
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DOI: https://doi.org/10.1007/BF02013611