Abstract
Sera from 48 cystic fibrosis patients from two hospitals were screened for antibody against rods, non-toxic macromolecular structures which share antigenic determinants withPseudomonas aeruginosa exotoxin A. A solid-phase radioimmunoassay employing (125I)-staphylococcal protein A was used to detect anti-rod IgG. Antibodies recognizing rods, exotoxin A, or both antigens, were demonstrated using a competitive radioimmunoassay in cystic fibrosis patient sera, and in sera from animals immunized with exotoxin A, rods, or infected withPseudomonas aeruginosa. Anti-rod titers of cystic fibrosis patients (1.07 to 14 x control serum levels) inversely correlated with aggregate clinical evaluation scores, and in most instances, with X-ray scores. Since rods are non-toxic and cross-reactive with exotoxin A, they may represent therapeutically useful antigens for producing immunity to exotoxin A.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Hoiby, N., Schoitz, P.: Pulmonary infections in cystic fibrosis. Acta Paediatrica Scandinavica 1982, Supplement 301: 1–132.
Iglewski, B. H., Kabat, D.: NAD-dependent inhibition of protein synthesis byPseudomonas aeruginosa toxin. Proceedings of the National Academy of Sciences of the United States of America 1975, 72: 2284–2288.
Woods, D. E., Iglewski, B. H.: Toxins ofPseudomonas aeruginosa: New perspectives. Reviews of Infectious Diseases 1983, 5, Supplement 4: S715-S721.
Middlebrook, J. L., Dorland, R. B.: Bacterial toxins: Cellular mechanisms of action. Microbiological Reviews 1984, 48: 199–221.
Pollack, M.: The virulence ofPseudomonas aeruginosa. Reviews of Infectious Diseases 1984, 6, Supplement 3: S617-S626.
Cross, A. S., Sadoff, J. C., Iglewski, B. H., Sokol, P. A.: Evidence for the role of toxin A in the pathogenesis of infection withPseudomonas aeruginosa in humans. Journal of Infectious Diseases 1980, 142: 538–546.
Cash, H. A., Straus, D. C., Bass, J. A.:Pseudomonas aeruginosa exoproducts as pulmonary virulence factors. Canadian Journal of Microbiology 1983, 29: 448–456.
Pollack, M., Longfield, R. N., Karney, W. W.: Clinical significance of serum antibody responses to exotoxin A and type-specific lipopolysaccharides in patients withPseudomonas aeruginosa infections. American Journal of Medicine 1983, 74: 980–987.
Klinger, J. D., Straus, D. C., Hilton, C. B., Bass, J. A.: Antibodies to proteases and exotoxin A ofPseudomonas aeruginosa in patients with cystic fibrosis: Demonstration by radioimmunoassay. Journal of Infectious Diseases 1978, 138: 49–58.
Klinger, K. W., Shuster, C. W.: A macromolecular structure produced byPseudomonas aeruginosa is recognized by antibody to exotoxin A. Infection and Immunity 1984, 43: 912–919.
Klinger, K. W., Klinger, J. D.: Cross-protective immunization againstPseudomonas aeruginosa challenge in mice. Cystic Fibrosis Club Abstracts 1982, 23: 15.
Taylor, N., Pollack, M.: Purification ofPseudomonas aeruginosa exotoxin A by affinity chromatography. Infection and Immunity 1978, 19: 66–70.
Doershuk, C. F., Matthews, L. W., Tucker, A. S., Nudelman, H., Eddy, G., Wise, M., Spector, S.: A five year clinical evaluation of a therapeutic program for patients with cystic fibrosis. Journal of Pediatrics 1964, 65: 677–693.
Cukor, G., Blacklow, N. R., Nowak, N. A., Rich, C. M., Braverman, L. E., Fischer, R. A.: Comparative analysis of serum antibody responses toPseudomonas aeruginosa exotoxin A by cystic fibrosis and intensive care unit patients. Journal of Clinical Microbiology 1983, 18: 457–462.
Bryan, L. E., Kureishi, A., Rabin, H. R.: Detection of antibodies toPseudomonas aeruginosa alginate extracellular polysaccharide in animals and cystic fibrosis patients by enzyme linked immunosorbent assay. Journal of Clinical Microbiology 1983, 18: 276–282.
Hancock, R. E. W., Mouat, E. C. A., Speert D. P.: Quantitation and identification of antibodies to outer-membrane proteins ofPseudomonas aeruginosa in sera of patients with cystic fibrosis. Journal of Infectious Diseases 1984, 149: 220–226.
Jagger, K. S., Robinson, D. L., Franz, M. N., Warren, R. L.: Detection by enzyme-linked immunosorbent assays of antibody specific forPseudomonas proteases and exotoxin A from cystic fibrosis patients. Journal of Clinical Microbiology 1982, 15: 1054–1058.
Pavlovskis, O. R., Edman, D. C., Leppla, S. H., Wretlind, B., Lewis, L. R., Martin, K. E.: Protection against experimentalPseudomonas aeruginosa infection in mice by active immunization with exotoxin A toxoids. Infection and Immunity 1981, 32: 681–689.
Cryz, S. J.:Pseudomonas aeruginosa infections. In: Germanier, R. (ed.): Bacterial vaccines. Academic Press, Inc., Orlando, FL, 1984, p. 317–351.
Bjorn, M. J., Vasil, M., Sadoff, J. C., Iglewski, B. H.: Incidence of exotoxin production byPseudomonas species. Infection and Immunity 1977, 16: 362–366.
Doring, G., Hoiby, N.: Longitudinal study of immune response toPseudomonas aeruginosa antigens in cystic fibrosis. Infection and Immunity 1983, 42: 197–201.
Hoiby, N.:Pseudomonas aeruginosa infection in cystic fibrosis. Acta Pathologica et Microbiologica Scandinavica (C) 1977, Supplement 262: 1–96.
Matthews, W. J., Jr., Williams, M., Oliphint, B., Geha, R., Colten, H. R.: Hypogammaglobinemia in patients with cystic fibrosis. New England Journal of Medicine 1980, 302: 245–249.
Klinger, J. D., Cash, H. A., Wood, R. E., Miler, J. J.: Protective immunization against chronicPseudomonas aeruginosa pulmonary infection in rats. Infection and Immunity 1983, 39: 1377–1384.
Diem, K., Lentner, C.: Scientific Tables. Ciba-Geigy, Ltd., Basel, 1970, p. 61.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Klinger, K.W., Shuster, C.W. & Klinger, J. Reaction of antibody in sera from cystic fibrosis patients with nontoxic forms ofPseudomonas aeruginosa exotoxin A. Eur. J, Clin. Microbiol. 4, 201–206 (1985). https://doi.org/10.1007/BF02013598
Issue Date:
DOI: https://doi.org/10.1007/BF02013598