Skip to main content
SpringerLink
Log in

Protection against fatalPseudomonas aeruginosa sepsis by immunization with smooth and rough lipopolysaccharides

  • Published:
European Journal of Clinical Microbiology Aims and scope Submit manuscript

Abstract

The protective capacity of various native and mutant lipopolysaccharide antigens against fatalPseudomonas aeruginosa burn wound sepsis was evaluated. Immunization with O-polysaccharide-deficient lipopolysaccharides derived fromEscherichia coli J5 orSalmonella typhi Ty 21a afforded substantial protection against only one of fivePseudomonas aeruginosa challenge strains of various serotypes. Immunization with both lipopolysaccharide antigens evoked antibody of the immunoglobulin G class which recognized lipopolysaccharide isolated from the challenge strain against which protection was noted. This was not seen for the remaining four challenge strains. Attempts to demonstrate cross-serotype protection using O-antigen-deficient and core-deficientPseudomonas aeruginosa lipopolysaccharide antigens was, for the most part, unsuccessful. In contrast, high levels of protection against all six serotypes of challenge strains were seen following immunization with homologous lipopolysaccharide.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Cross, A., Burke, J., Ducel, G., Harris, A., John, J., Johnson, D., Lew, M., MacMillan, B., Meers, P., Skalova, R., Wenzel, R., Tenney, J.: Nosocomial infections due toPseudomonas aeruginosa: Review of recent trends. Reviews of Infectious Diseases 1983, 5: 5837–5845.

    Google Scholar 

  2. Bodey, G. P., Bolivar, R., Fainstein, V., Jadeja, L.: Infections caused byPseudomonas aeruginosa. Reviews of Infectious Diseases 1983, 5: 279–313.

    PubMed  CAS  Google Scholar 

  3. Kreger, B. E., Craven, D. E., Carting, P. C., McCabe, W. R.: Gram-negative bacteremia. III. Reassessment of etiology, epidemiology and ecology in 612 patients. American Journal of Medicine 1980, 68: 332–343.

    Article  PubMed  CAS  Google Scholar 

  4. McGowan, J. E., Jr., Barnes, M. W., Finland, M.: Bacteremia at Boston City Hospital: occurrence and mortality during 12 selected years (1935–1972), with special reference to hospital-acquired cases. Journal of Infectious Diseases 1975, 132: 316–353.

    PubMed  Google Scholar 

  5. Bryan, C. S., Reynolds, K. L., Brennet, E. R.: Analysis of 1,186 episodes of gram-negative bacteremia in nonuniversity hospitals: the effects of antimicrobial therapy. Reviews of Infectious Diseases 1983, 4: 629–638.

    Google Scholar 

  6. Flick, M. R., Cluff, L. E.:Pseudomonas bacteremia. Review of 108 cases. American Journal of Medicine 1976, 60: 501–508.

    Article  PubMed  CAS  Google Scholar 

  7. Cryz, S. J., Jr.:Pseudomonas aeruginosa infections. In: Germanier, R. (ed.): Bacterial vaccines. Academic Press, New York, 1984, p. 317–351.

    Google Scholar 

  8. Young, L. S., Pollack, M.: Immunologic approaches to the prophylaxis and treatment ofPseudomonas aeruginosa infection. In: Sabbath, L. D., (ed.):Pseudomonas aeruginosa: the organism, diseases it causes, and their treatment. Hans Huber, Berne, 1980, p. 119–132.

    Google Scholar 

  9. Cryz, S. J., Jr., Fürer, E., Germanier, R.: Protection againstPseudomonas aeruginosa infection in a m urine burn wound sepsis model by passive transfer of antitoxin A, antielastase and anti-lipopolysaccharide. Infection and Immunity 1983, 39: 1072–1079.

    PubMed  CAS  Google Scholar 

  10. Cryz, S. J. Jr., Fürer, E., Germanier, R.: Protection against fatalPseudomonas aeruginosa bum wound sepsis by immunization with lipopolysaccharide and high-molecular-weight polysaccharide. Infection and Immunity 1984, 43: 795–799.

    PubMed  CAS  Google Scholar 

  11. Kazmierowski, J. A., Reynolds, H. Y., Kauffmann, J. C., Durbin, W. A., Graw, R. G., Jr., Devlin, H. B.: Experimental pneumonia due toPseudomonas aeruginosa in leukopenic dogs: prolongation of survival by combined treatment with passive antibody toPseudomonas and granulocyte transfusions. Journal of Infectious Diseases 1977, 135: 438–446.

    PubMed  CAS  Google Scholar 

  12. Sadoff, J. C., Futrovsky, S. L., Sidbery, H. F., Iglewski, B. H., Seid, R. C., Jr.: Detoxified lipopolysaccharideprotein conjugates. In: Robbins, J. B., Hill, J. C., Sadoff, J. C. (eds.): Bacterial vaccines. Thieme-Stratton, New York, 1982, p. 346–354.

    Google Scholar 

  13. Fisher, M. W.: A polyvalent human α-globulin immune toPseudomonas aeruginosa: passive protection of mice against lethal infection. Journal of Infectious Diseases 1977, 136 (Supplement): 181–185.

    Google Scholar 

  14. Crowder, J. G., Fisher, M. W., White, A.: Type-specific immunity in pseudomonas diseases. Journal of Laboratory and Clinical Medicine 1972, 79: 47–54.

    PubMed  CAS  Google Scholar 

  15. Habs, I.: Untersuchungen über die O-Antigene vonPseudomonas aeruginosa. Zeitschrift für Hygiene und Infektionskrankheiten 1957, 144: 218–228.

    PubMed  CAS  Google Scholar 

  16. Mikkelsen, O. S.: Serotyping ofPseudomonas aeruginosa. 2. Results of an O group classification. Acta Pathologica Microbiologica Scandinavia (B) 1970, 78: 163–175.

    CAS  Google Scholar 

  17. Ullmann, U., Schmulling, R. M.: Distribution ofPseudomonas aeruginosa serotypes in bacteriologic specimens and their aminoglycoside resistance. Zentralblatt für Bakteriologie (A) 1980, 247: 241–247.

    CAS  Google Scholar 

  18. Pier, G. B., Thomas, D. M.: Lipopoiysaccharide and high-molecular-weight polysaccharide serotypes ofPseudomonas aeruginosa. Journal of Infectious Diseases 1982, 145: 217–223.

    PubMed  CAS  Google Scholar 

  19. Ziegler, E. J., McCutchan, J. A., Douglas, H., Braude, A. I.: Prevention of lethalPseudomonas bacteremia with epimerase-deficientEscherichia coli antiserum. Transactions of the Association of American Physicians 1975: 101–108.

  20. Pennington, J. E., Hickey, W. F., Blackwood, L. L., Arnaut, M. A.: Active immunization with lipopoiysaccharidePseudomonas antigen for chronicPseudomonas bronchopneumonia in guinea pigs. Journal of Clinical Investigation 1981, 68: 1140–1148.

    Article  PubMed  CAS  Google Scholar 

  21. Meadow, P. M.: Wall and membrane structures in the genusPseudomonas. In: Clarke, P. H., Richmond, M. H. (eds.): Genetics and biochemistry ofPseudomonas. John Wily, London, 1975, p. 67–98.

    Google Scholar 

  22. Rowe, P. S. N., Meadow, P. M.: Structure of the core oligosaccharide from the lipopolysaccharide ofPseudomonas aeruginosa PAC 1R and its defective mutants. European Journal of Biochemistry 1983, 132: 329–337.

    Article  PubMed  CAS  Google Scholar 

  23. Koval, S. F., Meadow, P. M.: The isolation and characterization of lipopolysaccharide-defective mutants ofPseudomonas aeruginosa PAC 1. Journal of General Microbiology 1977, 98: 387–398.

    PubMed  CAS  Google Scholar 

  24. Cryz, S. J., Jr., Pitt, T. L., Furer, E., Germanier, R.: Role of lipopolysaccharide in virulence ofPseudomonas aeruginosa. Infection and Immunity 1984, 44: 508–513.

    PubMed  CAS  Google Scholar 

  25. Meadow, P. M., Wells, P. L.: Receptor sites for R-type pyocins and bacteriophage E79 in the core part of the lipopolysaccharide ofPseudomonas aeruginosa PAC 1. Journal of General Microbiology 1978, 108: 339–343.

    CAS  Google Scholar 

  26. Elbein, A. D., Heath, E. C.: The biosynthesis of cellwall lipopolysaccharide inEscherichia coli. I. The biochemical properties of a uridine diphosphate galactose 4-epimeraseless mutant. Journal of Biological Chemistry 1965, 240: 1919–1928.

    PubMed  CAS  Google Scholar 

  27. Germanier, R., Fürer, E.: Isolation and characterization ofGal E mutant Ty 21a ofSalmonella typhi: A candidate strain for a live oral typhoid vaccine. Journal of Infectious Diseases 1975, 131: 553–558.

    PubMed  CAS  Google Scholar 

  28. Björn, M. J., Iglewski, B. H., Ives, S. K., Sadoff, J. C., Vasil, M. J.: Effect of iron on yields of exotoxin A in cultures ofPseudomonas aeruginosa PA-103. Infection and Immunity 1978, 19: 785–791.

    PubMed  Google Scholar 

  29. Chester, I. R., Meadow, P. M., Pitt, T. L.: The relationship between the O-antigenic lipopolysaccharides and serological specificity in strains ofPseudomonas aeruginosa of different O-serotypes. Journal of General Microbiology 1973, 78: 305–318.

    PubMed  CAS  Google Scholar 

  30. Stieritz, D. D., Holder, I. A.: Experimental studies of the pathogenesis of infections due toPseudomonas aeruginosa: description of a burned mouse model. Journal of Infectious Diseases 1975, 131: 688–691.

    PubMed  CAS  Google Scholar 

  31. Mutharia, L. M., Crockford, G., Bogard, W. C., Jr., Hancock, R. E. W.: Monoclonal antibodies specific forEscherichia coli J5 lipopolysaccharide: cross-reaction with other gram-negative bacterial species. Infection and Immunity 1984, 45: 631–636.

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Swiss Serum and Vaccine Institute, P.O. Box 2707, 3001, Berne, Switzerland

    S. J. Cryz Jr., E. Fürer & R. Germanier

  2. Department of Biochemistry, University College London, London, UK

    P. M. Meadow

Authors
  1. S. J. Cryz Jr.
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. P. M. Meadow
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. E. Fürer
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. R. Germanier
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Cryz, S.J., Meadow, P.M., Fürer, E. et al. Protection against fatalPseudomonas aeruginosa sepsis by immunization with smooth and rough lipopolysaccharides. Eur. J, Clin. Microbiol. 4, 180–185 (1985). https://doi.org/10.1007/BF02013594

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02013594

Keywords

Search

Navigation