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Role of exoenzyme S in chronicPseudomonas aeruginosa lung infections

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Abstract

Exoenzyme S is an extracellular ADP-ribosyltransferase enzyme produced byPseudomonas aeruginosa. Mutants ofPseudomonas aeruginosa deficient in this enzyme have been shown to have reduced virulence in infections of burned mice. The contribution of exoenzyme S to the pathogenesis of chronic lung infections with this organism was evaluated by examining the incidence of exoenzyme S production byPseudomonas aeruginosa strains isolated from cystic fibrosis patients and comparing an exoenzyme S deficient mutant and its exoenzyme S producing parent in a rat chronic lung infection model. Of 51 isolates examined, 43 % produced detectable levels of exoenzyme S. While both the exoenzyme S deficient mutant and its parent strain were equally capable of colonizing and persisting in rat lungs, the exoenzyme S producing parent elicited a greater degree of lung damage. These data suggest that exoenzyme S contributes to the pathogenesis of chronic lung infections.

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Authors and Affiliations

  1. Department of Microbiology and Immunology, Oregon Health Sciences University, 97201, Portland, Oregon, USA

    T. I. Nicas, D. W. Frank, J. D. Lile & B. H. Iglewski

  2. Department of Pathology, Oregon Health Sciences University, 97201, Portland, Oregon, USA

    P. Stenzel

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  1. T. I. Nicas
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  2. D. W. Frank
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  3. P. Stenzel
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  4. J. D. Lile
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  5. B. H. Iglewski
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Nicas, T.I., Frank, D.W., Stenzel, P. et al. Role of exoenzyme S in chronicPseudomonas aeruginosa lung infections. Eur. J, Clin. Microbiol. 4, 175–179 (1985). https://doi.org/10.1007/BF02013593

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