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Use of transposon mutants to assess the role of exoenzyme S in chronic pulmonary disease due toPseudomonas aeruginosa

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Abstract

Among the potential virulence factors produced byPseudomonas aeruginosa there are two distinct ADP-ribosyl transferases, exotoxin A and exoenzyme S. The role of exoenzyme S inPseudomonas aeruginosa infection was studied using the rat chronic pulmonary infection model.Pseudomonas aeruginosa strain DG1 and an isogenic mutant of DG1 differing only in its capacity to produce exoenzyme S were employed in the study. BothPseudomonas aeruginosa strains tested established a chronic pulmonary infection in this model and organisms recovered from lung homogenates were phenotypically unaltered with respect to exoenzyme S production in vitro. The extent of the observed pathology was markedly greater with the strain producing exoenzyme S, indicating that exoenzyme S may play a role in the progressive pathology observed in chronic lung disease due toPseudomonas aeruginosa.

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Authors and Affiliations

  1. Department of Microbiology and Infectious Diseases, Faculty of Medicine, University of Calgary Health Sciences Centre, 3330 Hospital Drive N.W., T2N 4N1, Calgary, Alberta, Canada

    D. E. Woods & P. A. Sokol

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  1. D. E. Woods
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  2. P. A. Sokol
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Woods, D.E., Sokol, P.A. Use of transposon mutants to assess the role of exoenzyme S in chronic pulmonary disease due toPseudomonas aeruginosa . Eur. J, Clin. Microbiol. 4, 163–169 (1985). https://doi.org/10.1007/BF02013591

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