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Evaluation of novel antipseudomonal drugs using the serum bactericidal activity test

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Abstract

Serum bactericidal activity against Pseudomonas aeruginosa was determined in six volunteers 1 and 4 h after administration of 2 g ceftazidime, 4 g piperacillin, 500 mg imipenem, 80 mg tobramycin and four combinations of these agents. Ceftazidime produced the highest serum bactericidal titers, killing 100% and 86% of the 50 Pseudomonas aeruginosa strains tested after 1 and 4 h respectively at a serum dilution of 1∶8. Imipenem had lower serum bactericidal titers than ceftazidime, killing 88 % of the isolates after 1 h at a serum dilution of 1∶8. The combination showed only slightly higher titers. Killing curves were determined for nine strains of Pseudomonas aeruginosa using undiluted volunteer serum drawn 1 h after administration of the antibiotics. The combinations ceftazidime/tobramycin and piperacillin/ tobramycin exhibited higher killing activity than the single drugs. As the activity of the aminoglycosides could be underestimated on the basis of their low serum bactericidal titers, it is concluded that determination of these titers is inappropriate for evaluating the efficacy of the aminoglycosides.

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References

  1. Andriole, V. T.:Pseudomonas bacteremia: can antibiotic therapy improve survival? Journal of Laboratory Clinical Medicine 1979, 94: 196–200.

    Google Scholar 

  2. Baltch, A. L., Grifin, P. A.:Pseudomonas aeruginosa bacteremia: a clinical study of 75 patients. American Journal of Medical Science 1977, 274: 119–126.

    Google Scholar 

  3. Bodey, G. P., Jadeja, L., Elting, L.:Pseudomonas bacteremia: retrospective analysis of 410 episodes. Archives of Internal Medicine 1985, 145: 1621–1629.

    PubMed  Google Scholar 

  4. Machka, K., Dickert, H., Braveny, I.: In vitro activity of piperacillin compared with that of amipicillin, ticarcillin, azlocillin and mezlocillin. Arzneimittelforschung-Drug Research 1980, 30: 304–307.

    Google Scholar 

  5. Machka, K., Baierl, W., Braveny, I.: Comparative synergistic activity of netilmicin-piperacillin versus gentamicin-piperacillin. European Journal of Clinical Microbiology 1982, 1: 238–242.

    PubMed  Google Scholar 

  6. Reller, L. B., Stratton, C. W.: Serum dilution test for bactericidal activity. II Standardization and correlation with antimicrobial assays and susceptibility tests. Journal of Infectious Diseases 1977, 136: 196–204.

    PubMed  Google Scholar 

  7. Pennington, J. E., Stone, R. M.: Comparison of antibiotic regimens for treatment of experimental pneumonia due toPseudomonas. Journal of Infectious Diseases 1979, 140: 881–889.

    PubMed  Google Scholar 

  8. Lumisch, R. M., Norden, C. W.: Therapy of neutropenic rats infected withPseudomonas aeruginosa. Journal of Infectious Diseases 1976, 133: 538–547.

    PubMed  Google Scholar 

  9. Rusnak, M. G., Drake, T. A., Hackbarth, C. J., Sande, M. A.: Single versus combination antibiotic therapy for pneumonia due toPseudomonas aeruginosa in neutropenic guinea pigs. Journal of Infectious Diseases 1984, 149: 980–985.

    PubMed  Google Scholar 

  10. Anderson, E. T., Young, L. S., Hewitt, W. L.: Antimicrobial synergism in the therapy of gram-negative rod bacteremia. Chemotherapy 1978, 14: 45–54.

    Google Scholar 

  11. Klastersky, J., Meunier-Carpentier, F., Prevost, J. M.: Significance of antimicrobial synergism for the outcome of gram-negative sepsis. American Journal of Medical Science 1977, 273: 157–167.

    Google Scholar 

  12. Lagast, H., Zinner, S. H., Klastersky, J.: Serum bactericidal activity of moxalactam and cefotaxime with and without tobramycin againstPseudomonas aeruginosa andStaphylococcus aureus. Antimicrobial Agents and Chemotherapy 1981, 20: 539–541.

    PubMed  Google Scholar 

  13. Standiford, H. C., Drusano, G. L., Fitzpatrick, B., Tatem, B., Schimpff, S. C.: Bactericidal activity of ceftazidime in serum compared with that of ticarcillin combined with amikacin. Antimicrobial Agents and Chemotherapy 1984, 26: 339–342.

    PubMed  Google Scholar 

  14. Drake, T. A., Hackbarth, C. J., Sande, M. A.: Value of serum tests in combined drug therapy of endocarditis. Antimicrobial Agents and Chemotherapy 1983, 24: 653–657.

    PubMed  Google Scholar 

  15. Weinstein, M. P., Stratton, C. W., Ackley, A., Hawley, H. B., Robinson, P. A., Fisher, B. D., Alcid, D. V., Stephens, D. S., Reller, L. B.: Multicenter collaborative evaluation of a standardized serum bactericidal test as a prognostic indicator in infective endocarditis. American Journal of Medicine 1985, 78: 262–269.

    PubMed  Google Scholar 

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Authors and Affiliations

  1. Institute of Medical Microbiology, Technical University, Ismaningerstr. 22, 8000, Munich 80, FRG

    I. Braveny, K. Machka & D. Milatovic

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  1. I. Braveny
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  2. K. Machka
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  3. D. Milatovic
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Braveny, I., Machka, K. & Milatovic, D. Evaluation of novel antipseudomonal drugs using the serum bactericidal activity test. Eur. J, Clin. Microbiol. 5, 119–123 (1986). https://doi.org/10.1007/BF02013481

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  • DOI: https://doi.org/10.1007/BF02013481

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