Skip to main content
SpringerLink
Log in

Effect of ciprofloxacin on phagocytosis

  • Original Articles
  • Published:
European Journal of Clinical Microbiology Aims and scope Submit manuscript

Abstract

Certain aspects of the relationship between host defence mechanisms and the new quinoline derivative ciprofloxacin in comparison to norfloxacin and ofloxacin were studied. Ciprofloxacin did not affect chemotaxis of human polymophonuclear leucocytes in agarose. In leucocytes exposed to ciprofloxacin, norfloxacin and ofloxacin neither the chemiluminescent response to opsonized zymosan and formyl-methionyl-leucyl-phenylalanine nor the phagocytic or bactericidal activity was affected. However, killing ofStaphylococcus aureus by human polymorphonuclear leucocytes exposed to subinhibitory concentrations of ciprofloxacin was enhanced. The results show that the quinolines tested do not directly influence phagocytic cells, but a subinhibitory concentration can make bacteria more susceptible to phagocytosis and killing.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Milatovic, D.: Antibiotics and phagocytosis. European Journal of Clinical Microbiology 1983, 2: 414–425.

    PubMed  Google Scholar 

  2. Forsgren, A.: Comparative in vitro activity of three new quinolone antibiotics against recent clinical isolates. Scandinavian Journal of Infectious Diseases 1985, 17: 91–94.

    PubMed  Google Scholar 

  3. Traub, W. H.: Intraphagocytic bactericidal activity of bacterial DNA gyrase inhibitors againstSerratia marcescens. Chemotherapy 1984, 30: 379–386.

    PubMed  Google Scholar 

  4. Duncker, D., Ullmann, U.: Einfluß von Ciprofloxacin, Enoxacin und Norfloxacin auf die Phagozytose humaner neutrophiler Granulozyten, gemessen mit der Chemilumineszenz. Fortschritte der antimikrobiellen und antineoplastischen Chemotherapie 1984, 3–5: 633–635.

    Google Scholar 

  5. Bøyum, A.: Isolation of mononuclear cells and granulocytes from human blood. Scandinavian Journal of Clinical and Laboratory Investigation 1968, 21, Supplement 97: 77–89.

    PubMed  Google Scholar 

  6. Nelson, R. D., Quie, P. G., Simmons, R. L.: Chemotaxis under agarose: a new and simple method for measuring chemotaxis and spontaneous migration of human polymorphonuclear leukocytes and monocytes. Journal of Immunology 1975, 115: 1650–1656.

    Google Scholar 

  7. Forsgren, A., Schmeling, D.: Effect of antibiotics on chemotaxis of human leucocytes. Antimicrobial Agents and Chemotherapy 1977, 11: 580–584.

    PubMed  Google Scholar 

  8. Dahlgren, C., Stendahl, O.: Effect of in vitro preincubation of polymorphonuclear leucocytes on formylmethionylleucyl-phenyl-alanine induced chemiluminescence. Infection and Immunity 1982, 37: 34–39.

    PubMed  Google Scholar 

  9. Allred, C. D., Hill, H. R.: Effect of chemoattractants on chemiluminescence. Infection and Immunity 1978, 19: 833–838.

    PubMed  Google Scholar 

  10. Maaløe, O.: On the relation between alexin and opsonin. Einar Munksgaard, Copenhagen, 1946.

    Google Scholar 

  11. Hirsch, J. G., Strauss, B.: Studies on heat-labile opsonin in rabbit serum. Journal of Immunology 1964, 92: 145–154.

    Google Scholar 

  12. Forsgren, A., Schmeling, D.: Effect of antibiotics on chemotaxis of human leucocytes. Antimicrobial Agents and Chemotherapy 1977, 11: 580–584.

    PubMed  Google Scholar 

  13. Ohnishi, H., Kosuzume, H., Inaba, H., Okura, M., Mochizuki, H., Suzuki, Y., Fujii, R.: Effects of AC-1370, a new semisynthetic cephalosporin on phagocyte functions. Antimicrobial Agents and Chemotherapy 1983, 23: 874–880.

    PubMed  Google Scholar 

  14. Forsgren, A., Schmeling, D., Quie, P. G.: Effect of tetracycline on the phagocytic function of human leucocytes. Journal of Infectious Diseases 1974, 130: 412–415.

    PubMed  Google Scholar 

  15. Alien, R. C., Losse, L. D.: Phagocytic activation of a luminol-dependent chemiluminescence in rabbit alveolar and peritoneal macrophages. Biochemical and Biophysical Research Communications 1976, 69: 245–252.

    PubMed  Google Scholar 

  16. Hatch, G. E., Gardner, D. E., Menzel, D. B.: Chemiluminescence of phagocytic cells caused by N-formylmethionylpeptides. Journal of Experimental Medicine 1978, 147: 182–195.

    PubMed  Google Scholar 

  17. Douglas, S. D., Quie, P. G. (ed.): Investigation of phagocytes in disease. Churchill Livingstone, Edinburgh, 1981, p. 53–60.

    Google Scholar 

  18. De Chatelet, L. R., Long, G. D., Shirley, P. S., Bass, D. A., Thomas, M. J., Henderson, F. W., Cohen, M. S.: Mechanism of the luminol-dependent chemiluminescence of human neutrophils. Journal of Immunology 1982, 129: 1589–1593.

    Google Scholar 

  19. McDonald, P. J., Wetherall, B. L., Pruul, H.: Post-antibiotic leucocyte enhancement. Increased susceptibility of bacteria pretreated with antibiotics to activity of leucocytes. Reviews of Infectious Diseases 1981, 3: 38–44.

    PubMed  Google Scholar 

  20. Horne, D., Tomasz, A.: Hypersusceptibility of penicillin-treated group B streptococci to bactericidal activity of human polymorphonuclear leucocytes. Antimicrobial Agents and Chemotherapy 1981, 19: 745–753.

    PubMed  Google Scholar 

  21. Alexander, J. W., Good, R. A.: Effect of antibiotics on the bactericidal activity of human leucocytes. Journal of Laboratory and Clinical Medicine 1968, 71: 971–983.

    PubMed  Google Scholar 

  22. Milatovic, D.: Effect of subinhibitory antibiotic concentrations on the phagocytosis ofStaphylococcus aureus. European Journal of Clinical Microbiology 1982, 1: 97–101.

    PubMed  Google Scholar 

  23. Elliot, G. R., Peterson, P. K., Verbrugh, H. A., Freiberg, M. R., Hoidal, J. R., Quie, P. C.: Influence of subinhibitory concentrations of penicillin, cephalothin, and clindamycin onStaphylococcus aureus growth in human phagocytic cells. Antimicrobial Agents and Chemotherapy 1982, 22: 781–784.

    PubMed  Google Scholar 

  24. Michel, J., Ferner, M., Borinski, R., Kornberg, Z., Bergner-Rabinowitz, S., Ginsbutg, I.: Effects of subminimal inhibitory concentrations of chloramphenicol, erythromycin and penicillin on group A streptococci. European Journal of Clinical Microbiology 1982, 1: 375–380.

    PubMed  Google Scholar 

  25. Gemmel, C. G., Peterson, P. K., Schmeling, D., Mathews, J., Quie, P. G.: Antibiotic-induced modification ofBacterioides fragilis and its susceptibility to phagocytosis by human polymorphonuclear leucocytes. European Journal of Clinical Microbiology 1983, 2: 327–334.

    PubMed  Google Scholar 

  26. Milatovic, D., Braveny, I., Verhoef, J.: Clindamycin enhances opsonization ofStaphylococcus aureus. Antimicrobial Agents and Chemotherapy 1983, 24: 413–417.

    PubMed  Google Scholar 

  27. Andreana, A., Perna, P., Utili, R., Dilillo, M., Ruggiero, G.: Increased phagocytosis and killing ofEscherichia coli treated with subinhibitory concentrations of cefamandole and gentamicin in isolated rat livers. Antimicrobial Agents and Chemotherapy 1984, 25: 182–186.

    PubMed  Google Scholar 

  28. Bassaris, H. P., Lianou, P. E., Votta, E. G., Skoutelis, A. T., Papavassiliou, J. T.: Enhancement of polymorphonuclear leucocyte function against gram-positive aerobic organisms grown in the presence of lincomycin. Infection 1984, 12: 369–371.

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Medical Microbiology, University of Lund, Malmö General Hospital, S-21401, Malmö, Sweden

    A. Forsgren & P. -I. Bergkvist

Authors
  1. A. Forsgren
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. P. -I. Bergkvist
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Forsgren, A., Bergkvist, P.I. Effect of ciprofloxacin on phagocytosis. Eur. J, Clin. Microbiol. 4, 575–578 (1985). https://doi.org/10.1007/BF02013398

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02013398

Keywords

Search

Navigation