European Journal of Clinical Microbiology

, Volume 5, Issue 6, pp 639–642 | Cite as

Diagnosis of pneumococcal pneumonia by detection of antigen in saliva

  • A. Krook
  • H. Fredlund
  • H. Holmberg


A sandwich enzyme-linked immunosorbent assay was used for the detection of pneumococcal C polysaccharide in saliva samples from patients with radiologically verified pneumonia. In 16 of 29 patients (55 %) with pneumococcal pneumonia, as verified by conventional culture methods, the antigen was present in saliva specimens. The test was negative in 35 of 36 patients (97 %) with non-pneumococcal pneumonia and in all saliva samples from a control group, consisting of 25 patients with no signs of respiratory disease. It is concluded that the detection of pneumococcal C polysaccharide in saliva offers a valuable complement to conventional diagnostic methods for pneumonoccal pneumonia.


Internal Medicine Pneumonia Polysaccharide Immunosorbent Assay Respiratory Disease 
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  1. 1.
    Schieiner, A., Digranes, A., Myking, O., Sohlberg, C. O.: Wert der transtrachealen Aspiration bei bronchopulmonalen Infektionen im Erwachsenenalter. Infection 1973, 1: 137–143.CrossRefPubMedGoogle Scholar
  2. 2.
    Davidson, M., Tempest, B., Palmer, D. L.: Comparison of sputum, transtracheal aspiration and lung puncture. Journal of the American Medical Association 1976, 235: 158–163.CrossRefPubMedGoogle Scholar
  3. 3.
    Kalin, M.: Bacteremic pneumococcal pneumonia: value of culture of nasopharyngeal specimens and examination of washed sputum specimens. European Journal of Clinical Microbiology 1982, 1: 394–395.CrossRefPubMedGoogle Scholar
  4. 4.
    Gardner, P. S.: Rapid virus diagnosis. Journal of General Virology 1977, 36: 1–29.PubMedCrossRefGoogle Scholar
  5. 5.
    Hendley, J. O., Sande, M. A., Stewart, P. M., Gwaltney, J. M.: Spread ofStreptococcus pneumoniae in families. I: Carriage rates and distribution of types. Journal of Infectious Diseases 1975, 132: 55–61.PubMedGoogle Scholar
  6. 6.
    Dowling, J. N., Sheehe, P. R., Feldman, H. A.: Pharyngeal pneumococcal acquisitions in normal families: a longitudinal study. Journal of Infectious Diseases 1971, 124: 9–17.PubMedGoogle Scholar
  7. 7.
    Webster, L. T., Hughes, T. P.: The epidemiology of pneumococcus infection. The incidence and spreads of pneumococci in the nasal passage and throats of healthy persons. Journal of Experimental Medicine 1931, 53: 535–552.CrossRefGoogle Scholar
  8. 8.
    Murray, P. R., Washington, J. A.: Microscopic and bacteriologic analysis of expectorated sputum. Mayo Clinic Proceedings 1975, 50: 339–344.PubMedGoogle Scholar
  9. 9.
    Bartlett, J. G., Finegold, S. M.: Bacteriology of expectorated sputum with quantitative culture and wash technique compared to transtracheal aspirates. American Review of Respiratory Disease 1978, 117: 1019–1027.PubMedGoogle Scholar
  10. 10.
    Monroe, P. W., Muchmore, H. G., Felton, F. G., Pirtle, J. K.: Quantitation of microorganisms in sputum. Applied Microbiology 1969, 18: 214–220.PubMedGoogle Scholar
  11. 11.
    Holmberg, H., Holme, T., Krook, A., Olsson, T., Sjöberg, L., Sjögren, A.-M.: Detection of C polysaccharide inStreptococcus pneumoniae in the sputa of pneumonia patients by an enzyme-linked immunosorbent assay. Journal of Clinical Microbiology 1985, 22: 111–115.PubMedGoogle Scholar
  12. 12.
    Brundish, D. E., Baddiley, J.: Pneumococcal C-substance, a ribitol teichoic acid containing choline phosphate. Biochemistry Journal 1968, 110: 573–582.Google Scholar
  13. 13.
    Jennings, H. J., Lugowski, C., Young, N. M.: Structure of the complex polysaccharide C-substance fromStreptococcus pneumoniae type 1. Biochemistry 1980, 19: 4712–4719.CrossRefPubMedGoogle Scholar
  14. 14.
    Holmberg, H., Krook, A., Sjögren, A.-M.: Determination of antibodies to pneumococcal C polysaccharide in patients with community-acquired pneumonia. Journal of Clinical Microbiology 1985, 202: 808–814.Google Scholar
  15. 15.
    Bornstein, D. L., Schiffman, G., Bernheimer, H. P., Austrian, R.: Capsulation of pneumococcus with soluble C-like polysaccharide. Journal of Experimental Medicine 1968, 128: 1385–1400.CrossRefPubMedGoogle Scholar
  16. 16.
    Pedersen, K. P., Henrichsen, J., Sörensen, U. S., Nielsen, J. L.: Anti-C-carbohydrate antibodies after pneumococcal vaccination. Acta Pathologica Microbiologica et Immunologica Scandinavica (C) 1982, 90: 353–355.Google Scholar
  17. 17.
    Bowden, G. H., Ellwood, D. C., Hamilton, J. R.: Microbial ecology of the oral cavity, In: Alexander, M. (ed.): Advances in microbial ecology. Plenum Publishing, London, 1979, p. 135–217.Google Scholar
  18. 18.
    Zepp, H. D., Hodes, H. L.: Antigenic relation of type bHaemophilus influenzae to type 29 and type 6 pneumococci. Proceedings of the Society for Experimental Biology and Medicine 1943, 52: 315–317.Google Scholar

Copyright information

© Vieweg Publishing 1986

Authors and Affiliations

  • A. Krook
    • 1
  • H. Fredlund
    • 2
  • H. Holmberg
    • 3
  1. 1.Department of Infectious Diseases, Roslagstull HospitalKarolinska InstituteStockholmSweden
  2. 2.Department of Infectious DiseasesÖrebro Medical Center HospitalÖrebroSweden
  3. 3.Department of Clinical Microbiology and ImmunologyÖrebro Medical Center HospitalÖrebroSweden

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