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European Journal of Clinical Microbiology

, Volume 5, Issue 6, pp 639–642 | Cite as

Diagnosis of pneumococcal pneumonia by detection of antigen in saliva

  • A. Krook
  • H. Fredlund
  • H. Holmberg
Article

Abstract

A sandwich enzyme-linked immunosorbent assay was used for the detection of pneumococcal C polysaccharide in saliva samples from patients with radiologically verified pneumonia. In 16 of 29 patients (55 %) with pneumococcal pneumonia, as verified by conventional culture methods, the antigen was present in saliva specimens. The test was negative in 35 of 36 patients (97 %) with non-pneumococcal pneumonia and in all saliva samples from a control group, consisting of 25 patients with no signs of respiratory disease. It is concluded that the detection of pneumococcal C polysaccharide in saliva offers a valuable complement to conventional diagnostic methods for pneumonoccal pneumonia.

Keywords

Internal Medicine Pneumonia Polysaccharide Immunosorbent Assay Respiratory Disease 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Schieiner, A., Digranes, A., Myking, O., Sohlberg, C. O.: Wert der transtrachealen Aspiration bei bronchopulmonalen Infektionen im Erwachsenenalter. Infection 1973, 1: 137–143.CrossRefPubMedGoogle Scholar
  2. 2.
    Davidson, M., Tempest, B., Palmer, D. L.: Comparison of sputum, transtracheal aspiration and lung puncture. Journal of the American Medical Association 1976, 235: 158–163.CrossRefPubMedGoogle Scholar
  3. 3.
    Kalin, M.: Bacteremic pneumococcal pneumonia: value of culture of nasopharyngeal specimens and examination of washed sputum specimens. European Journal of Clinical Microbiology 1982, 1: 394–395.CrossRefPubMedGoogle Scholar
  4. 4.
    Gardner, P. S.: Rapid virus diagnosis. Journal of General Virology 1977, 36: 1–29.PubMedCrossRefGoogle Scholar
  5. 5.
    Hendley, J. O., Sande, M. A., Stewart, P. M., Gwaltney, J. M.: Spread ofStreptococcus pneumoniae in families. I: Carriage rates and distribution of types. Journal of Infectious Diseases 1975, 132: 55–61.PubMedGoogle Scholar
  6. 6.
    Dowling, J. N., Sheehe, P. R., Feldman, H. A.: Pharyngeal pneumococcal acquisitions in normal families: a longitudinal study. Journal of Infectious Diseases 1971, 124: 9–17.PubMedGoogle Scholar
  7. 7.
    Webster, L. T., Hughes, T. P.: The epidemiology of pneumococcus infection. The incidence and spreads of pneumococci in the nasal passage and throats of healthy persons. Journal of Experimental Medicine 1931, 53: 535–552.CrossRefGoogle Scholar
  8. 8.
    Murray, P. R., Washington, J. A.: Microscopic and bacteriologic analysis of expectorated sputum. Mayo Clinic Proceedings 1975, 50: 339–344.PubMedGoogle Scholar
  9. 9.
    Bartlett, J. G., Finegold, S. M.: Bacteriology of expectorated sputum with quantitative culture and wash technique compared to transtracheal aspirates. American Review of Respiratory Disease 1978, 117: 1019–1027.PubMedGoogle Scholar
  10. 10.
    Monroe, P. W., Muchmore, H. G., Felton, F. G., Pirtle, J. K.: Quantitation of microorganisms in sputum. Applied Microbiology 1969, 18: 214–220.PubMedGoogle Scholar
  11. 11.
    Holmberg, H., Holme, T., Krook, A., Olsson, T., Sjöberg, L., Sjögren, A.-M.: Detection of C polysaccharide inStreptococcus pneumoniae in the sputa of pneumonia patients by an enzyme-linked immunosorbent assay. Journal of Clinical Microbiology 1985, 22: 111–115.PubMedGoogle Scholar
  12. 12.
    Brundish, D. E., Baddiley, J.: Pneumococcal C-substance, a ribitol teichoic acid containing choline phosphate. Biochemistry Journal 1968, 110: 573–582.Google Scholar
  13. 13.
    Jennings, H. J., Lugowski, C., Young, N. M.: Structure of the complex polysaccharide C-substance fromStreptococcus pneumoniae type 1. Biochemistry 1980, 19: 4712–4719.CrossRefPubMedGoogle Scholar
  14. 14.
    Holmberg, H., Krook, A., Sjögren, A.-M.: Determination of antibodies to pneumococcal C polysaccharide in patients with community-acquired pneumonia. Journal of Clinical Microbiology 1985, 202: 808–814.Google Scholar
  15. 15.
    Bornstein, D. L., Schiffman, G., Bernheimer, H. P., Austrian, R.: Capsulation of pneumococcus with soluble C-like polysaccharide. Journal of Experimental Medicine 1968, 128: 1385–1400.CrossRefPubMedGoogle Scholar
  16. 16.
    Pedersen, K. P., Henrichsen, J., Sörensen, U. S., Nielsen, J. L.: Anti-C-carbohydrate antibodies after pneumococcal vaccination. Acta Pathologica Microbiologica et Immunologica Scandinavica (C) 1982, 90: 353–355.Google Scholar
  17. 17.
    Bowden, G. H., Ellwood, D. C., Hamilton, J. R.: Microbial ecology of the oral cavity, In: Alexander, M. (ed.): Advances in microbial ecology. Plenum Publishing, London, 1979, p. 135–217.Google Scholar
  18. 18.
    Zepp, H. D., Hodes, H. L.: Antigenic relation of type bHaemophilus influenzae to type 29 and type 6 pneumococci. Proceedings of the Society for Experimental Biology and Medicine 1943, 52: 315–317.Google Scholar

Copyright information

© Vieweg Publishing 1986

Authors and Affiliations

  • A. Krook
    • 1
  • H. Fredlund
    • 2
  • H. Holmberg
    • 3
  1. 1.Department of Infectious Diseases, Roslagstull HospitalKarolinska InstituteStockholmSweden
  2. 2.Department of Infectious DiseasesÖrebro Medical Center HospitalÖrebroSweden
  3. 3.Department of Clinical Microbiology and ImmunologyÖrebro Medical Center HospitalÖrebroSweden

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