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Calcified Tissue Research

, Volume 26, Issue 1, pp 51–59 | Cite as

Effects of fluoride on in vitro calcification of tendon matrix

  • C. L. Wadkins
  • R. A. Luben
Article

Summary

Ca2+ and Pi uptake induced in vitro by a collagenous matrix derived from bovine tendon is inhibited by 1×10−6 to 2×10−5M NaF and stimulated by 2×10−5 to 2×10−3M NaF. Fluoride uptake occurs only over the latter concentrtion range. The uptake of Ca2+, Pi, and F−1 progresses toward a limiting extent at which the molar Ca/P and Ca/F values are 1.6 to 1.7 and 4.5 to 5.7, respectively. Although the matrix-bound mineral, previously formed in the absence of NaF, readily undergoes dissolution when exposed to a Ca2+- and P-free medium of pH<7.4, the bound mineral phase formed in the presence of NaF does not. We conclude that fluoroapatite is the primary matrix-bound mineral. The uptake of fluoride, Ca2+. and Pi by both uncalcified and previously calcified matrices is inhibited by methylenediphosphonate and by phosphonoacetate as is calcification in the absence of NaF. Kinetic studies indicate that formation of a CaP complex precedes the uptake of F−1 and suggest that F−1 and OH−1 compete for interation with the CaP complex during the calcification process. We concluded that fluoroapatite formation induced by the collagenous matrix occurs by a multistep pathway comparable to that proposed previously for hydroxyapatite formation.

Key words

Collagen Calcification Fluoroapatite Nucleation Inhibitors 

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References

  1. 1.
    Rich, C., Feist, E.: The action of fluoride on bone. In: Fluoride in medicine, p. 70. Hans Huber Publishers, Bern 1970Google Scholar
  2. 2.
    Eanes, E.D., Zipkin, I., Harper, R.A., Posner, A.S.: Smallangle X-ray diffraction analysis of the effect of fluoride on human bone apatite. Arch. Oral Biol.10, 1961–1973 (1965)Google Scholar
  3. 3.
    Gedalia, I., Zipkin, I.: The role of fluoride in bone structure. St. Louis: W.H. Green, Inc. 1973Google Scholar
  4. 4.
    Hicks, E.P., Ramp, W.K.: The effects of fluoride on the mineralization of embryonic chick tibiae in organ culture. Calcif. Tissue Res.17, 205–217 (1975)PubMedGoogle Scholar
  5. 5.
    Messer, H.H., Armstrong, W.D., Singer, L.: Fluoride, parathyroid homrone, and calcitonin: Inter-relationships in bone calcium metabolism. Calcif. Tissue Res.13, 217–225 (1973)PubMedGoogle Scholar
  6. 6.
    Messer, H.H., Armstrong, W.D., Singer, L.: Fluoride, parathyroid homrone, and calcitonin: Effects on metabolic processes involved in bone resorption. Calcif. Tissue Res.13, 227–233 (1973)PubMedGoogle Scholar
  7. 7.
    Neuman, W.F., Neuman, M.W., Main, E.R., Leary, J.O., Smith, F.A.: The surface chemistry of bone. II. Fluoride deposition. J. Biol. Chem.187, 655–661 (1950)PubMedGoogle Scholar
  8. 8.
    Simpson, D.R.: Problems of the composition and structure of the bone minerals. Clin. Orthop.86, 260–286 (1972)PubMedGoogle Scholar
  9. 9.
    Brown, W.E., Smith, J.P., Lehr, J.R., Frazier, A.W.: Crystallographic and chemical relations between octacalcium phosphate and hydroxyapatite. Nature196, 1050–1055 (1962)Google Scholar
  10. 10.
    Taves, D.R., Neuman, W.F.: Factors controlling calcification in vitro: Fluoride and magnesium. Arch. Biochem. Biophys.108, 390–397 (1964)PubMedGoogle Scholar
  11. 11.
    Meyer, J.L., Nancollas, G.H.: Effects of stannous and fluoride ions on the rate of crystal growth of hydroxyapatite. J. Dent. Res.51, 1444–1450 (1972)Google Scholar
  12. 12.
    Wadkins, C.L., Luben, R., Thomas, M., Humphrey, R.: Physical biochemistry of calcification. Clin. Orthop.99, 246–266 (1974)PubMedGoogle Scholar
  13. 13.
    Thomas, W.C., Jr., Tomita, A.: Mineralization of human and bovine tissues in vitro. Am. J. Pathol.51, 621–628 (1967)PubMedGoogle Scholar
  14. 14.
    Luben, R.A., Sherman, J.K., Wadkins, C.L.: Studies of the mechanism of biological calcification. IV. Ultrastructural analysis of calcifying tendon matrix. Calcif. Tissue Res.11, 39–55 (1973)PubMedGoogle Scholar
  15. 15.
    Luben, R.A., Wadkins, C.L.: Studies of the relationship of proton production and calcification of tendon matrix in vitro. Biochemistry10, 2183–2189 (1971)PubMedGoogle Scholar
  16. 16.
    Gomori, G.: A modification of the colorimetric phosphorus determination for use with the phosphoelectric colorimeter. J. Lab. Clin. Med.27, 955–960 (1941–42)Google Scholar
  17. 17.
    Jethi, R.K., Inlow, C.W., Wadkins, C.L.: Studies of the mechanism of biological calcification I. Kinetic properties of the in vitro calcification of collagen-containing matrix. Calcif. Tissue Res.6, 81–92 (1970)PubMedGoogle Scholar
  18. 18.
    Wadkins, C.L.: Experimental factors that influence collagen calcification in vitro. Calcif. Tissue Res.2, 214–228 (1968)PubMedGoogle Scholar
  19. 19.
    Megregian, S.: Rapid spectrophotometric determination of fluoride with Zirconium-Eriochrome Cyanine R Lake. Anal. Chem.26, 1161–1166 (1954)Google Scholar
  20. 20.
    Francis, M.D., Russell, R.G.G., Fleisch, H.: Diphosphonates inhibit formation of calcium phosphate crystals in vitro and pathological calcification in vivo. Science165, 1264–1266 (1969)PubMedGoogle Scholar
  21. 21.
    Posner, A.S.: The structure, properties, and formation of calcium phosphates in hard tissue. In: Cellular mechanisms for calcium transfer and homeostatis (Nicholas, G., Jr., Wasserman, R.H., eds.), pp. 3–23. New York: Academic Press 1971Google Scholar
  22. 22.
    Termine, J.D., Posner, A.S.: Calcium phosphate formation in vitro. I. Factors affecting initial phase separation. Arch. Biochem. Biophys.140, 307–317 (1970)PubMedGoogle Scholar
  23. 23.
    Termine, J.D., Peckauskas, R.A., Posner, A.S.: Calcium phosphate formation in vitro. II. Effects of environment of amorphous-crystalline transformation. Arch. Biochem. Biophys.140, 318–325 (1970)PubMedGoogle Scholar
  24. 24.
    West, V.C., Storey, E.: The in vitro effect of fluoride and tetracycline on phase transformation of a calcium phosphate. Calcif. Tissue Res.9, 207–215 (1972)PubMedGoogle Scholar
  25. 25.
    Hasvold, O., Dahm, S.: The effect of fluoride on the transformation of amorphous calcium phosphate into crystalline apatite. Calcif. Tissue Res. [Suppl.],22, 425–427 (1977)PubMedGoogle Scholar

Copyright information

© Springer-Verlag 1978

Authors and Affiliations

  • C. L. Wadkins
    • 1
  • R. A. Luben
    • 1
  1. 1.Department of BiochemistryUniversity of Arkansas College of MedicineLittle Rock

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