Abstract
To evaluate a non-marketed research prototype of a solid-phase enzyme immunoassay for detection of herpes simplex virus in genital lesions, 154 clinical specimens were collected from 127 men and 27 women with symptoms suggestive of herpes simplex virus infection (erythema, vesicles, ulcers and crustae). The samples were tested using the assay and cultures on four monolayers of human embryonic lung fibroblasts and Vero cells. When the culture was used as reference method, sensitivity was 76.9% and specificity 100% (prevalence 42.4%). Comparison of results by patient group showed that sensitivity was highest in material from patients with vesicles and ulcers. The highest sensitivity was obtained in specimens which developed a cytopathological effect within 48 h and in specimens with three or four positive cell cultures. These findings suggest that the assay is more successful in specimens with high virus titres. The enzyme immunoassay was found to be a rapid, moderately sensitive, highly specific test for detection of herpes simplex virus from genital lesions, but the usefulness of the assay is limited and culture methods should be preferred.
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Moseley, R. C., Corey, L., Benjamin, D., Winter, C., Remington, M. L.: Comparison of viral isolation, direct immunofluorescence and indirect immunoperoxidase techniques for detection of genital herpes simplex virus infection. Journal of Clinical Microbiology 1981, 13: 913–918.
Vestergaard, B. F., Jensen, O.: Diagnosis and typing of herpes simplex virus in clinical specimens by the enzymelinked immunosorbent assay. In: Nahmias, A. J. (ed.): The human herpes viruses: an interdisciplinary perspective. Elsevier, New York, 1981, p. 391–394.
Land, S. A., Skurrie, I. J., Gilbert, G. L.: Rapid diagnosis of herpes simplex virus infections by enzyme-linked immunosorbent assay. Journal of Clinical Microbiology 1984, 19: 865–869.
Morgan, M. A., Smith, T. F.: Evaluation of an enzymelinked immunosorbent assay for the detection of herpes simplex virus antigen. Journal of Clinical Microbiology 1984, 19: 730–732.
Warford, A. L., Levy, R. A., Rekrut, K. A.: Evaluation of a commercial enzyme-linked immunosorbent assay for detection of herpes simplex virus antigen. Journal of Clinical Microbiology 1984, 20: 490–493.
Sewell, D. L., Horn, S. A.: Evaluation of a commercial enzyme-linked immunosorbent assay for the detection of herpes simplex virus. Journal of Clinical Microbiology 1985, 21: 457–458.
Vecchio, T. J.: Predictive value of a single diagnostic test in unselected populations. New England Journal of Medicine 1966, 274: 1171–1173.
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van Ulsen, J., Dumas, A.M., Wagenvoort, J.H.T. et al. Evaluation of an enzyme immunoassay for detection of herpes simplex viras antigen in genital lesions. Eur. J, Clin. Microbiol. 6, 410–413 (1987). https://doi.org/10.1007/BF02013096
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DOI: https://doi.org/10.1007/BF02013096