Abstract
The purpose of this investigation was to develop a serological procedure for rapid identification of the following fiveBacteroides species:Bacteroides distasonis, Bacteroides fragilis, Bacteroides ovatus, Bacteroides uniformis, andBacteroides vulgatus. The outer membrane fractions were assayed using SDS-polyacrylamide slab gel electrophoretic techniques. The species-specific protein band from each species as well as the group-specific protein were purified and used to immunize rabbits. The species-specific and the group-specific antisera produced were used to develop an indirect enzyme-linked immunosorbent assay to detect the species-specific and group-specific protein from the outer membrane of five reference species. The sensitivity and specificity of the procedure were evaluated by indirect ELIS A methodology using 506 clinical isolates of organisms in this group, ten other species of anaerobic gram-negative bacilli, and three species of aerobic gram-negative bacilli. Each species evaluated yielded unique outer membrane protein patterns, suggesting the potential for development of a rapid, species-specific diagnostic procedure.
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References
Finegold, S. M.: Anaerobic bacteria in human disease. Academic Press, New York, 1977.
Willis, A. T.: Aerobic bacteriology: clinical and laboratory practice. Butterworths, London, 1977.
Wren, M. W. D., Baldwin, A. W. F., Eldon, C. P., Sanderson, P. J.: The anaerobic culture of clinical specimens: a 14-month study. Journal of Medical Microbiology 1977, 10: 49–61.
Holdeman, L. V., Cato, E. P., Moore, W. E. C.: Anaerobic laboratory manual. Virginia Polytechnic Institute and State University, Blacksburg, Va., 1977.
Duerden, B. I.: The isolation and identification ofBacteroides species from the normal human faecal flora. Journal of Medical Microbiology 1980, 13: 69–78.
Finegold, S. M., Flora, D. J., Atterbery, H. R., Sutler, V. L.: Faecal bacteriology of colonie polyp patients and control patients. Cancer Research 1975, 35: 3407–3417.
Collee, J. G., Brown, R., Poxton, I. R., Fraser, A. G.: Current approaches to the classification, chracterization and typing of pathogenic anaerobic bacteria. Scandinavian Journal of Infectious Disease Supplement 1982, 35: 17–21.
Lennette, E. H., Balows, A., Hausler, W. J., Truant, J. P. (ed.): Manual of clinical microbiology. American Society for Microbiology, Washington, D.C., 1980.
Poxton, I. R., Ip, M. K. Y.: The cell-surface antigens ofBacteriodes vulgatus. Journal of General Microbiology 1981, 126: 102–109.
Laemmli, U. K.: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970, 227: 680–685.
Lowry, O. H., Rosebrough, N. J., Farr, A. L., Randall, R. J.: Protein measurement with the folin phenol reagent. Journal of Biological Chemistry 1951, 193: 265–266.
Hames, B. D.: Introduction to polyacrylamide gel electrophoresis. In: Hames, B. D., Rickwood, D. (ed.): Gel electrophoresis of proteins: a practical approach. IRL Press Limited, London, 1981.
Osterman, L. A.: Electrophoresis of proteins. In: Osterman, L. A. (ed.): Methods of protein and nucleic acid research. Volume 1. Springer-Verlag, Berlin, 1984, p. 30–102.
Osterman, L. A.: Fundamentals of immunochemistry. In: Osterman, L. A. (ed.): Methods of protein and nucleic acid research. Volume 2. Springer-Verlag, Berlin, 1984, p. 4–38.
Weir, D. M.: Handbook of experimental immunology. Blackwell Scientific, Oxford, 1978.
Voller, A.: Heterogenous enzyme-immunoassay and their applications. In: Maggio, E. T. (ed.): Enzymeimmunoassay. CRC Press Inc., Boca Raton, Florida, 1980, p. 181–196.
Eng vall, E., Perlmann, P.: Enzyme-linked immunosorbent assay, ELISA. III. Quantitation of specific antibody by enzyme-labelled anti-immunoglobulin in antigen-coated tubes. Journal of Immunology 1972, 109: 129–135.
Johnson, J. L.: Taxonomy ofBacteroides. I. Deoxyribonucleic acid homologies amongBacteroides Fragilis and other saccharolyticBacteroides species. International Journal of Systemic Bacteriology 1978, 28: 245–256.
Poxton, I. R., Brown, R.: Sodium dodecyl sulphatepolyacrylamide gel electrophoresis of cell-surface proteins as an aid to the identification ofBacteroides fragilis group. Journal of General Microbiology 1979, 12: 211–217.
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Marshall, R., Pongparit, S. & Kaufman, A.K. Serological evaluation of the outer membrane protein complexes of five saccharolytic intestinalBacteroides species. Eur. J, Clin. Microbiol. 6, 395–401 (1987). https://doi.org/10.1007/BF02013093
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DOI: https://doi.org/10.1007/BF02013093