Abstract
Pentafluorobenzyl and pentafluoropropionyl/pentafluorobenzyl esters of bacterial fatty acids were analysed by capillary gas chromatography using both flame ionization and electron capture detection. No differences between the relative peak areas of the various fatty acids were observed as regards the two detectors used except that response of the electron capture detector to the hydroxy acid derivatives exceeded that to the non-hydroxy acid derivatives by 20%. Use of the described derivatives in combination with electron capture detection yields chromatograms comparable with those obtained by analysis of methyl esters using flame ionization detection but with superior sensitivity.
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References
Larsson, L., Odham, G.: Injection principles in capillary gas Chromatographic analysis of bacterial fatty acids. Journal of Microbiological Methods 1984, 3: 77–82.
Alley, C. C., Brooks, J. B., Kellogg, D. S.: Electron capture gas-liquid chromatographic-mass spectral identification of acids produced byNeisseria meningitidis in a defined medium. Journal of Clinical Microbiology 1979, 9: 97–102.
Rosenfeld, J. M., Hammerberg, O., Orvidas, M. C.: Simplified methods for preparation of microbial fatty acids for analysis by gas chromatography with electron capture detection. Journal of Chromatography 1986, 378: 9–16.
Gilbart, J.: Detection ofLegionella spp. in water samples by electron capture gas chromatography. In: Thornsberry, C. (ed.): Proceedings, 2nd International Symposium on Legionella. American Society for Microbiology, Washington, DC, 1984, p. 296–298.
Lambert, M. A., Moss, C.W.: Comparison of the effects of acid and base hydrolyses on hydroxy and cyclopropane fatty acids in bacteria. Journal of Clinical Microbiology 1983,18: 1370–1377.
Fox, A., Morgan, S. L., Hudson, J. R., Zhu, Z. T., Lau, P. Y.: Capillary gas Chromatographic analysis of alditol acetates of neutral and amino sugars in bacterial cell walls. Journal of Chromatography 1983, 256: 429–438.
Sonesson, A., Larsson, L., Jimenez, J.: Use of pentafluorobenzyl and pentafluoropropionyl — pentafluoro- benzyl esters of bacterial fatty acids for gas chromatographic analysis with electron capture detection. Journal of Chromatography 1987, 417: 366–370.
Brondz, I., Olsen, I.: Chemotaxonomy at a cross-roads? Gas chromatographic analyses of a single colony from the bacteriumHaemophilus aphrophilus. Journal of Chromatography 1986, 374: 119–124.
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Larsson, L., Sonesson, A. & Jimenez, J. Ultrasensitive analysis of microbial fatty acids using gas chromatography with electron capture detection. Eur. J, Clin. Microbiol. 6, 729–731 (1987). https://doi.org/10.1007/BF02013086
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DOI: https://doi.org/10.1007/BF02013086