European Journal of Clinical Microbiology

, Volume 6, Issue 6, pp 724–728 | Cite as

Gas chromatographic comparison of peptone yeast glucose and gas liquid chromatography growth media for anaerobic bacteria

  • E. Holst
  • L. Larsson
Current Topic: Article


The growth media peptone yeast extract glucose broth and gas liquid chromatography broth were compared with respect to their suitability for anaerobic bacteria, using 63 strains belonging to 12 species. After incubation for 24 and 48 h the broth cultures were subjected to viable counts and gas Chromatographic analysis of short-chain alcohols and fatty acids in head-space vapours and ether extracts. Head-space analysis was superior to the ether extraction method for detecting the rapidly eluting short-chain alcohols. The gas liquid chromatography medium provided superior growth and contained fewer compounds that interfered with the Chromatographic analyses. Whereas incubation in gas liquid chromatography medium for 24 h gave characteristic chromatograms for all but one of the studied species, most species required 48 h of incubation in peptone yeast extract glucose medium. Gas liquid chromatography broth is the preferred substrate for use in gas Chromatographic identification of anaerobic bacteria.


Anaerobic Bacterium Medium Peptone Chromatographic Comparison Peptone Yeast Extract Glucose Peptone Yeast Glucose 
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  1. 1.
    Holdeman, L. V., Cato, E. P., Moore, W.E.C.: Anaerobe laboratory manual. Virginia Polytechnic Institute and State University, Blacksburg, Virginia, 1977.Google Scholar
  2. 2.
    Mayhew, J.W., Gorbach, S.L.: Internal standards for gas chromatographic analysis of metabolic end products from anaerobic bacteria. Applied and Environmental Microbiology 1977, 33: 1002–1003.PubMedGoogle Scholar
  3. 3.
    Bohannon, T.E., Manius, G., Mamaril, F., Li Wen, L.-F.: Quantitative methods for the gas chromatographic characterization of acidic fermentation by-products of anaerobic bacteria. Journal of Chromatographic Science 1978, 16: 28–35.PubMedGoogle Scholar
  4. 4.
    Bricknell, K. S., Finegold, S.M.: A simple, rapid method to process and assay fatty acids and alcohols by gas chromatography. Analytical Biochemistry 1973, 51: 23–31.PubMedGoogle Scholar
  5. 5.
    Larsson, L., Holst, E.: Feasibility of automated headspace gas chromatography in identification of anaerobic bacteria. Acta Pathologica, Microbiologica et Immunologica Scandinavica (B) 1982, 90: 125–130.Google Scholar
  6. 6.
    Larsson, L., Holst, E., Gemmell, C. G., Mårdh, P.-A.: Characterization ofClostridium difficile and its differentiation fromClostridium sporogenes by automatic headspace-gas-chromatography. Scandinavian Journal of Infectious Diseases 1980, Supplement 22: 37–40.Google Scholar
  7. 7.
    Larsson, L., M⇘rdh, P.-A., Odham, G.: Analysis of amines and other bacterial products by head-space gas chromatography. Acta Pathologica, Microbiologica et Immunologica (B) 1978, 86: 207–213.Google Scholar
  8. 8.
    Larsson, L., Mårdh, P.-A., Odham, G.: Detection of alcohols and volatile fatty acids by head-space gas chromatography in identification of anaerobic bacteria. Journal of Clinical Microbiology 1978, 7: 23–27.PubMedGoogle Scholar
  9. 9.
    Drasar, B. S., Goddard, P., Heaton, S., Peach, S., West, B.: Clostridia isolated from faeces. Journal of Medical Microbiology 1976, 9: 63–71.PubMedGoogle Scholar
  10. 10.
    Moss, C. W., Nunez-Montiel, O. L.: Analysis of short chain acids from bacteria by gas-liquid chromatography with a fused-silica capillary column. Journal of Clinical Microbiology 1982, 15: 308–311.PubMedGoogle Scholar

Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1987

Authors and Affiliations

  • E. Holst
    • 1
  • L. Larsson
    • 1
  1. 1.Department of Medical MicrobiologyUniversity of LundLundSweden

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