Abstract
Extracts of representative mycobacteria were examined by thin-layer chromatography for glycosylphenolphthiocerol dimycocerosates. The glycolipid typical of Mycobacterium bovis was also found in Mycobacterium africanum and Mycobacterium microti,but it was absent in Mycobacterium bovis AN 5.Mycobacterium gastri strains contained a glycolipid which was chromatographically similar to that in Mycobacterium kansasii.Representatives of Mycobacterium marinum produced a distinct glycolipid type, and one strain had major amounts of a more polar variant. The sugar moieties of purified lipids, including that from Mycobacterium leprae,were identified by thin-layer chromatography of methyl glycosides in acid methanolysates.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Minnikin, D.E., Goodfellow, M.: Lipid composition in the classification and identification of acid-fast bacteria. In: Goodfellow, M., Board, R.G. (ed.): Microbiological classification and identification. Academic Press, London, 1980, p. 189–256
Dobson, G., Minnikin, D.E., Minnikin, S.M., Parlett, J. H., Goodfellow, M., Ridell, M., Magnusson, M.: Systematic analysis of complex mycobacterial lipids. In: Goodfellow, M., Minnikin, D.E. (ed.): Chemical methods in bacterial systematics. Academic Press, London, 1985, p. 237–265.
Minnikin, D. E., Minnikin, S.M., Parlett, J.H., Goodfellow, M.: Mycolic acid patterns of some rapidlygrowing species ofMycobacterium. Zentralblatt für Bakteriologie. Mikrobiologie und Hygiene (A) 1985, 259: 446–460.
Tisdall, P.A., Roberts, G.D., Anhalt, J.P.: Identification of clinical isolates of mycobacteria with gas-liquid chromatography alone. Journal of Clinical Microbiology 1979, 10:506–514.
Daffé, M., Lanéelle, M.A., Asselineau, C., Lévy-Frébault, V., David, H.: Intérêt taxonomique des acides gras des mycobactéries: proposition d'une methode d'analyse. Annales de Microbiologie (B) 1983, 134: 241–256.
Minnikin, D.E., Dobson, G., Goodfellow, M., Magnusson, M., Ridell, M.: Distribution of some mycobacterial waxes based on the phthiocerol family. Journal of General Microbiology 1985, 131: 1375–1381.
Minnikin, D.E., Dobson, G., Parlett, J. H.: Extraction and chromatographic analysis of characteristic mycobacterial lipids. In: Habermehl, K.-O. (ed.): Rapid methods and automation in microbiology and immunology. Springer, Berlin, 1985, p. 274–282.
Davidson, L.A., Draper, P., Minnikin, D.E.: Studies on the mycolic acids from the walls ofMycobaterium microti. Journal of General Microbiology 1982, 128: 823–828.
Jacin, H., Mishkin, A.R.: Separation of carbohydrates on borate-impregnated silica gel G plates. Journal of Chromatography 1965, 18: 170–173.
Gastambide-Odier, M.: Variantes de mycosides caractérisées par des résidus glycosidiques substitués par des chaînes acyles- I: Spectres de masse des mycosides G′ et A′ peracetyles. Organic Mass Spectrometry 1973, 7: 845–860.
Gastambide-Odier, M.: Variantes de mycosides caractérisées par des résidues glycosidiques substitués par des chaînes acyles. Nature mycolique des mycosides G′. European Journal of Biochemistry 1973, 33: 81–86.
Demarteau-Ginsburg, H., Lederer, E.: Sur la structure chimique du mycoside B. Biochimica et Biophysica Acta 1963,70:442–451.
Villé, C., Gastambide-Odier, M.: Le 3-O-méthyl-Lrhamnose, sucre isolé du mycoside G deMycobacterium marinum. Carbohydrate Research 1970, 12: 97–107.
Gastambide-Odier, M., Villé, C.: Desoxysucres isolés du mycoside A: Identification des dérivés acétylés des méthyl 2,4-di-O-méthyl-rhamnopyranoside, 2-O-méthyl rhamnofuranoside, 3-O-méthyl-rhamnofuranoside, 2-O-méthyl-fucopyranoside et 3-O-méthyl-fucofuranoside. Bulletin de la Société de Chimie Biologique 1970, 47: 2047–2065.
Hunter, S.W., Fuji wara, T., Brennan, P.J.: Structure and antigenicity of the major specific glycolipid antigen ofMycobacterium leprae. Journal of Biological Chemistry 1982, 257: 15072–15078.
Goodfellow, M., Wayne, L.G.: Taxonomy and nomenclature. In: Ratledge, C., Stanford, J.L. (ed.): The biology of the mycobacteria. Volume 1. Academic Press, London, 1982, p. 471–521.
Wayne, L.G.: Mycobacterial speciation. In: Kubica, G. P., Wayne, L.G. (ed.): The mycobacteria: a sourcebook. Marcel Dekker, New York, 1984, p. 25–65.
Magnusson, M.: A comparative study ofMycobacterium gastri andMycobacterium kansasii by delayed type skin reactions in guinea pigs. American Review of Respiratory Disease 1971, 104: 377–384.
Norlin, M., Lind, A., Ouchterlony, O.: A serologically based taxonomic study ofMycobacterium gastri. Zeitschrift für Immunitätsforschung. Allergie und Klinische Immunologie 1969, 137: 241–248.
Stanford, J.L., Grange, J.M.: The meaning and structure of species as applied to mycobacteria. Tubercle 1974, 55: 143–152.
Minnikin, D.E., Parlett, J.H., Dobson, G., Goodfellow, M., Magnusson, M., Ridell, M.: Lipid profiles of members of theMycobacterium tuberculosis complex. In: Casai, M. (ed.): Mycobacteria of clinical interest. Elsevier, Amsterdam, 1986, p. 75–78.
Richards, W.D.: Phage susceptibilities ofMycobacterium bovis isolates. Annali Sclavo, Sienna, 1975, 17: 618–622.
Minnikin, D.E., Parlett, J.H., Magnusson, M., Ridell, M., Lind, A.: Mycolic acid patterns of representatives ofMycobacterium bovis BCG. Journal of General Microbiology 1984, 130: 2733–2736.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Minnikin, D.E., Dobson, G., Parlett, J.H. et al. Analysis of dimycocerosates of glycosylphenolphthiocerols in the identification of some clinically significant mycobacteria. Eur. J, Clin. Microbiol. 6, 703–707 (1987). https://doi.org/10.1007/BF02013082
Issue Date:
DOI: https://doi.org/10.1007/BF02013082