European Journal of Clinical Microbiology

, Volume 6, Issue 6, pp 664–667 | Cite as

Evaluation of three methods for typing herpes simpex viras

  • F. Salmerón
  • P. Pérez Breña
  • J. M. Rojas
  • R. Hernáez Lobo
  • E. Tabarés


Three methods of typing herpes simplex virus were compared. A total of 111 clinical isolates obtained from patients not treated with antivirals and seven resistant mutants selected in vitro were tested by immunofluorescence assay using antibodies against herpes simplex virus types 1 and 2. Twenty-nine isolates were also studied by restriction endonuclease analysis. The sensitivity of isolates and resistant mutants to (E)-5-(2-bromovinyl)-2′-deoxyuridine was determined. Although a clear difference between the 50 % inhibitory dose for type 1 and type 2 isolates was observed, some drug-resistant mutants might be misidentified by this method.


Internal Medicine Restriction Endonuclease Herpes Simplex Virus Type Clinical Isolate 
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Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1987

Authors and Affiliations

  • F. Salmerón
    • 1
  • P. Pérez Breña
    • 2
  • J. M. Rojas
    • 2
  • R. Hernáez Lobo
    • 1
  • E. Tabarés
    • 3
  1. 1.Carretera de Majadahonda a PozueloCentro Nacional de FarmacobiologíaMajadahonda, MadridSpain
  2. 2.Virologia e Inmunología SanitariasCentro Nacional de MicrobiologíaMajadahonda, MadridSpain
  3. 3.Facultad de MedicinaUniversidad Autónoma de MadridMadridSpain

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