Evaluation of three methods for typing herpes simpex viras
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Three methods of typing herpes simplex virus were compared. A total of 111 clinical isolates obtained from patients not treated with antivirals and seven resistant mutants selected in vitro were tested by immunofluorescence assay using antibodies against herpes simplex virus types 1 and 2. Twenty-nine isolates were also studied by restriction endonuclease analysis. The sensitivity of isolates and resistant mutants to (E)-5-(2-bromovinyl)-2′-deoxyuridine was determined. Although a clear difference between the 50 % inhibitory dose for type 1 and type 2 isolates was observed, some drug-resistant mutants might be misidentified by this method.
KeywordsInternal Medicine Restriction Endonuclease Herpes Simplex Virus Type Clinical Isolate
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- 2.Lonsdale, D. M.: A rapid technique for distinguishing herpes simplex virus type 1 from type 2 by restriction enzyme technology. Lancet 1979, i: 849–852.Google Scholar
- 6.Mayo, D. R.: Differentiation of herpes simplex virus types 1 and 2 by sensitivity to (E)-5-(2-bromovinyl)-2′-deoxyuridine. Journal of Clinical Microbiology 1980, 15: 733–736.Google Scholar
- 8.Salmerón, F., Hernáez, R.: In vitro sensitivity of herpes simplex virus types 1 and 2 isolates against 9-β-D-arabinofuranosyladenine, 9-((2-hydroxy-1-(hydroxy-methyl) ethoxy) methyl) guanine and 9-(2-hydroxyethoxymethyl) guanine. Microbiologia 1986, 2: 11–16.Google Scholar
- 9.Gardner, P. S., McQuillin, J.: Rapid virus diagnosis. Application of immunofluorescence. Butterworths, London, 1980, p. 106–109.Google Scholar
- 11.Swierkosz, E. M., Arens, M. Q., Rivetna, K. A.: Problems associated with (E)-5-(2-bromovinyl)-2′-deoxyuridine for typing herpes simplex virus. Journal of Clinical Microbiology 1985, 3: 459–461.Google Scholar
- 12.Reno, J. M., Lee, L. F., Boezi, J. A.: Inhibition of herpesvirus replication and herpesvirus induced deoxyribonucleic acid polymerase by phosphonophormate. Antimicrobial Agents and Chemotherapy 1986, 2: 188–192.Google Scholar