Abstract
A miniaturized 2-h system for detection of lactosenegative potentially enteropathogenic bacteria on the basis of nine enzymatic tests was evaluated. The system was challenged with 210 strains of lactose-negative and lactose-positive species grown on MacConkey agar. Specific and constant patterns were found for Salmonella, Shigella A-C,Yersinia enterocolitica, Aeromonas spp. and Vibrio cholerae. Shigella sonnei and Hafnia alvei had an identical pattern, likewise Plesiomonas shigelloides,halophilic vibrios and Pseudomonas aeruginosa.
Similar content being viewed by others
References
Levine, M. M.:Escherichia coli that cause diarrhea: enterotoxigenic, enteropathogenic, enteroinvasive, enterohemorrhagic, and enteroadherent. Journal of Infectious Diseases 1987, 155: 377–389.
Haldane, D. J. M., Damm, M. A. S., Anderson, J. D.: Improved biochemical screening procedure for small clinical laboratories for Vero (Shiga-like)-toxin-producing strains ofEscherichia coli 0157: H7. Journal of Clinical Microbiology 1986, 24: 652–653.
Harvey, S. M.: Hippurate hydrolysis byCampylobacter fetus. Journal of Clinical Microbiology 1980, 11: 435–437.
Greene, L. C., Appelbaum, P. C., Kellogg, J. A.: Evaluation of a two-hour method for screening pathogens from stool specimens. Journal of Clinical Microbiology 1984, 20: 285–287.
Teti, G., Burdash, N. M., Zamboni. C., Fava, C., Tomasello, F., Mastroeni, P.: Evaluation of a rapid method to exclude the presence of certain enteric pathogens in stool specimens. Journal of Clinical Microbiology 1984, 20: 1049–1052.
James, A. L., Yeoman, P., Rasburn, J. W., Ng, M.: Sensitive reagents for detection of indole production by bacteria. Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene (A) 1986, 262: 195–202.
Kilian, M., Bülow, P.: Rapid diagnosis ofEnterobacteriaceae. I: Detection of bacterial glycosidases. Acta Pathologica et Immunologica Scandinavica (B) 1976, 84: 245–251.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
von Graevenitz, A., Zollinger-Iten, J. & Monget, D. Two-hour miniaturized system for detection of enteropathogenic bacteria in stool cultures. Eur. J, Clin. Microbiol. 6, 661–664 (1987). https://doi.org/10.1007/BF02013065
Issue Date:
DOI: https://doi.org/10.1007/BF02013065