European Journal of Clinical Microbiology

, Volume 6, Issue 6, pp 659–661 | Cite as

Enzyme immunoassay and enzyme-linked fluoroimmunoassay for detection ofChlamydia trachomatis antigen

  • M. Leinonen
  • P. Saikku
  • M. Nurminen
  • A. Lassus
Notes

Abstract

An enzyme immunoassay (EIA) and an enzymelinked fluoroimmunoassay (ELFIA) utilizing monoclonal antibody to major outer membrane protein ofChlamydia trachomatisL2 were developed for rapid detection of chlamydial antigen in clinical samples. The EIA and ELFIA could detect levels of purified chlamydial outer membrane protein as low as 1.0 and 0.2 ng/ml respectively. However, when EIA and ELFIA were compared to chlamydial isolation using 160 patient samples, the sensitivity rate was 68 % and 85% respectively. The sensitivity of the antigen detection method might be increased by simply using less diluted samples than in the present study. Chlamydial antigen was also demonstrated by EIA and ELFIA in 15% and 23% of culture-negative samples. The reason for these false-positive findings remains undetermined.

Keywords

Monoclonal Antibody Detection Method Clinical Sample Patient Sample Enzyme Immunoassay 

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Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1987

Authors and Affiliations

  • M. Leinonen
    • 1
  • P. Saikku
    • 2
  • M. Nurminen
    • 1
  • A. Lassus
    • 3
  1. 1.National Public Health InstituteHelsinkiFinland
  2. 2.Department of VirologyUniversity of HelsinkiHelsinkiFinland
  3. 3.Department of DermatovenerologyUniversity Central HospitalHelsinkiFinland

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