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An in vitro and in vivo investigation of mellitate and ethane-1-hydroxy-1, 1-diphosphonate in calcium phosphate systems

Calcified Tissue Research volume 23, pages 53–60 (1977)Cite this article

Summary

Comparisons of mellitate (MA) and ethane-1-hydroxy-1, 1-diphosphonate (EHDP) have been carried out in studies of enamel etching, calcium phosphate crystal growth and animal calculus deposition. In enamel etch studies at pH 5, 6, or 7 and after treatment times of 5 or 170 min, EHDP was less damaging to enamel surfaces than MA as determined by scanning electron microscopy, grazing angle electron diffraction, and quantitative etch solution analyses. Both MA and EHDP inhibited hydroxyapatite crystal growth, although EHDP was more effective than MA. The formation of a tricalcium mellitate surface phase is suggested as the basis of the MA crystal growth effect on apatite. Both MA and EHDP also inhibited rat calculus formation, but EHDP was more effective than MA. The relation between crystal growth inhibition, surface phase solubility, and anti-calculus activity is discussed and a generalized principal for determining an effective inhibitor of calculus is suggested.

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Affiliations

  1. Miami Valley Laboratories 2N142, USA

    Marion D. Francis, Candice L. Slough & Richard P. Oertel

  2. Winton Hill Technical Center, The Procter & Gamble Company, P.O. Box 39175, 45247, Cincinnati, Ohio, USA

    William W. Briner

Authors
  1. Marion D. Francis
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  2. Candice L. Slough
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  3. William W. Briner
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  4. Richard P. Oertel
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Francis, M.D., Slough, C.L., Briner, W.W. et al. An in vitro and in vivo investigation of mellitate and ethane-1-hydroxy-1, 1-diphosphonate in calcium phosphate systems. Calc. Tis Res. 23, 53–60 (1977). https://doi.org/10.1007/BF02012766

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  • DOI: https://doi.org/10.1007/BF02012766

Key words

  • Mellitic acid
  • Ethane-1-hydroxy-1,1-diphosphonate
  • Hydroxyapatite
  • Calculus
  • Instrumentation