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Trypanosoma cruzi: metabolic labeling of trypomastigote sialoglycolipids

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  • Biochemistry and Biophysics, Metabolism, Neurobiology, Pharmacology
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Summary

Trypomastigote forms (infective) ofTrypanosoma cruzi incorporate (3H)-palmitic acid and D-(3H)-galactose into glycolipids. Palmitic acid-labeled acidic glycolipids were partially hydrolyzed with neuraminidase. The labeling of these compounds when the intact cell surface was labeled with galactose oxidase plus NaB3H4 indicates the membrane location of the sialoglycolipids.

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Authors and Affiliations

  1. Departamento de Química Orgánica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, 1428, Buenos Aires, Argentina

    A. S. Couto & R. M. de Lederkremer

  2. Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, C.P. 20780, São Paulo, Brazil

    B. Zingales & W. Colli

Authors
  1. A. S. Couto
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  2. B. Zingales
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  3. R. M. de Lederkremer
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  4. W. Colli
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Additional information

This investigation received financial support from SECYT and CONICET to RML; CNPq, FINEP, and UNDP/World Bank/ WHOSSpecial Programme for Research and Training in Tropical Diseases to WC; and FAPESP to BZ and WC. ASC is a fellow from FAPESP.

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Couto, A.S., Zingales, B., de Lederkremer, R.M. et al. Trypanosoma cruzi: metabolic labeling of trypomastigote sialoglycolipids. Experientia 41, 736–738 (1985). https://doi.org/10.1007/BF02012572

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  • DOI: https://doi.org/10.1007/BF02012572

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