Calcified Tissue Research

, Volume 10, Issue 1, pp 128–135 | Cite as

Mineralization in the chick embryo

I. Monohydrogen phosphate and carbonate relationships during maturation of the bone crystal complex
  • Edmund D. Pellegrino
  • Robert M. Biltz
Original Papers

Abstract

The chemical and physical maturation of the bone salt was studied by serial observations on its stoichiometric and infrared characteristics in avian bone from early embryonic mineral deposition to full maturity after hatching. The sequence of chemical transformations in the developing bone showed most predominantly an inverse relationship between acid phosphate and carbonate, coincident with the formation of CO3-apatite. The data are consistent with the view that CO 3 2− is substituted for HPO 4 2− in the synthesis of CO3-apatite in bone.

Key words

Bone Embryo Calcification Phosphate Pyrophosphate Carbonate Apatite 

Résumé

La maturation chimique et physique du sel osseux est étudiée par l'obervation des caractéristiques stoichiométriques et aux infra-rouges d'os embryonnaire d'oiseaux, du début jusqu'à la fin de la croissance adulte après éclosion. La succession des transformations chimiques de l'os en voie de développement montre surtout un rapport inverse entre le phosphate acide et le carbonate, ainsi que la formation de CO3-apatite. Ces résultats semblent indiquer que le CO 3 2− est substitué au HPO 4 2− au cours de la synthèse du CO3-apatite de l'os.

Zusammenfassung

Die chemischen und physikalischen Umwandlungen zum stabilen Knochensalz in Hühnerknochen wurden anhand serienmäßiger Beobachtungen seiner stöchiometrischen Zusammensetzung und seiner infraroten Charakteristika untersucht; diese Beobachtungen erstreckten sich über die Zeit der frühen embryonalen Mineralablagerung bis zur vollständigen Reife nach dem Ausbrüten. Die Sequenz der chemischen Umwandlungen im sich entwickelnden Knochen zeigte hauptsächlich ein entgegengesetztes Verhältnis zwischen saurem Phosphat und Carbonat, das mit der Bildung von Carbonatapatit zusammenfällt. Diese Resultate weisen darauf hin, daß HPO 4 2− bei der Synthese von Carbonatapatit im Knochen durch CO 3 2− substituiert wird.

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Copyright information

© Springer-Verlag 1972

Authors and Affiliations

  • Edmund D. Pellegrino
    • 1
  • Robert M. Biltz
    • 1
  1. 1.Department of Medicine, Health Sciences CenterState University of New YorkStony BrookUSA

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