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Quantitative histochemistry of cartilage

Alkaline phosphatase and glucose-6-phosphate dehydrogenase activity in different zones of rachitic rat cartilage during healing

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Abstract

1. The quantitative distribution of alkaline phosphatase and glucose-6-phosphate dehydrogenase in proliferating and hypertrophic zones of rachitic rat cartilage during healing induced by vitamin D administration or starvation is presented. 2. Alkaline phosphatase activity in the hypertrophic zone is about three times greater than that of the proliferating zone of the normal weanling rat and approximately four times greater in the rachitic rats. 3. Following a single dose of vitamin D (8000 I. U.) there was a significant elevation in alkaline phosphatase activity of the proliferating zone. A less significant increase was observed in the proliferating zone of rats which had breen fasted 48 hours. 4. There was a significant decrease in the alkaline phosphatase activity of the central region of hypertrophic zone of cartilage after vitamin D treatment. No such decrease was observed in the fasted rats. 5. In the normal weanling rat glucose-6-phosphate dehydrogenase (G-6-PDH) activity was greater in the proliferating zone as compared to the hypertrophic zone. In the untreated rachitic rat the enzyme content was about the same in the two zones. 6. Following vitamin D treatment there was an appreciable decrease in the G-6-PDH activity. No such decrease was observed in the fasted rat.

Résumé

  1. 1.

    La distribution quantitative de phosphatase alcaline et de déshydrogénas eglucose-6-phosphate dans les zones hypertrophiques, en voie de prolifération du cartilage de rats rachitiques, pendant la cicatrisation induite par l'administration de vitamin D ou par jeun prolongé, est étudiée.

  2. 2.

    L'activité en phosphatase alcaline de la zone hypertrophique est environ triple de celle de la zone de prolifération du rat sevré normal et environ quadruple de celle des rats rachitiques.

  3. 3.

    Apres administration d'une seule dose de vitamine D (8000 unités), il se produit une augmentation significative de l'activité en phosphatase alcaline de la a zone en voie de prolifération. Une augmentation moins nette est observée dans la zone de prolifération de rats ayant jeuné 48 heures.

  4. 4.

    On observe une diminution significative de l'activité en phosphatase alcaline de la partie centrale de la zone hypertrophique de cartilage aprés traitement a la vitamine D. Une telle diminution n'a pas été observée chez le rat qui jeûne.

  5. 5.

    Chez le rat sevré normal, l'activité en déshydrogénase glucose-6-phosphate (G-6-PDH) est plus élevée dans la zone de prolifération que dans la zone hypertrophique. Chez le rat rachitique non traité, le contenu en enzyme est à peu pres identique dans les deux zones.

  6. 6.

    Apres traitement a la vitamine D, on note une décroissance nette de l'activité en G-6-PDH. Une telle décroissance n'est pas observée chez le rat qui jeûne.

Zusammenfassung

  1. 1.

    Diese Arbeit beschreibt die quantitative Verteilung der alkalischen Phosphatase und der Glucose-6-Phosphat-Dehydrogenase in proliferierenden und hypertrophischen Zonen von rachitischem Rattenknorpel während der durch Vitamin D-Gabe hervorgerufenen Heilung oder während der Mangeldiät.

  2. 2.

    Die Aktivität der alkalischen Phosphatase in der hypertrophischen Zone war etwa dreimal so groß wie in der proliferierende Zone der normalen entwöhnten Ratte und etwa viermal so groß bei den rachitischen Ratten.

  3. 3.

    Nach einer einmaligen Vitamin D-Gabe (8000 i.E) erfolgte eine signifikante Erhöhung der alkalischen Phosphatasen-Aktivität in der proliferierenden Zone. Eine weniger signifikante Erhöhung wurde in der proliferierenden Zone von Ratten festgestellt, welche 48 Std gefastet hatten.

  4. 4.

    Nach Vitamin D-Behandlung erfolgte eine signifikante Abnahme der alkalischen Phosphatasen-Aktivität in der mittleren Region der hypertrophischen Zone des Knorpels. Bei den fastenden Ratten wurde keine solche Abnahme beobachtet.

  5. 5.

    Bei der normalen entwöhnten Ratte war die Aktivität der Glucose-6-Phosphat-Dehydrogenase (G-6-PDH) größer in der proliferierenden als in der hypertrophischen Zone. Bei der unbehandelten rachitischen Ratte war der Enzymgehalt in beiden Zonen etwa gleich.

  6. 6.

    Nach Vitamin D-Behandlung erfolgte eine merkliche Abnahme der G-6-PDH-Aktivität. Bei der fastenden Ratte wurde keine solche Abnahme beobachtet.

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Authors and Affiliations

  1. Institute of Origin, Department of Anatomy, University of Minnesota, Medical School, 262 Jackson Hall, 55455, Minneapolis, Minnesota, USA

    Padmakar K. Dixit (Professor of Anatomy)

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  1. Padmakar K. Dixit
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Dixit, P.K. Quantitative histochemistry of cartilage. Calc. Tis Res. 10, 49–57 (1972). https://doi.org/10.1007/BF02012535

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  • DOI: https://doi.org/10.1007/BF02012535

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