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Dynamic interactions ofPseudomonas aeruginosa and bacteriophages in lake water

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Abstract

The persistence and interaction between newly isolated strains ofPseudomonas aeruginosa and resident bacteriophages indigenous to a freshwater environment was monitored over 45 days in lake water microcosms. The interaction between susceptible and resistant bacteria with pure phage (UT1) particles or a mixed phage population (M1) was investigated by following temporal changes in host density, phage-to-bacteria ratio (PBR), and the appearance of apparent prophage carriers within the host population. Decay rates of the phage (UT1) ranged from 0.054 hour−1 in natural water to 0.027 hour−1 in filtered lake water. About 45% of sensitive bacteria incubated with phase UT1 were pseudolysogenic within 12 hours of incubation in natural lake water. This process was delayed until 72 hours in the steile lake water control, suggesting that host-phage interaction is promoted in the presence of a viable natural microbial community. Phage UT1 appeared to stabilize the density of host bacteria in lake water at a level of 104 colony-forming units (cfu) ml−1. Bacterial coexistence with the mixed phage (M1) population resulted in an oscillating equilibrium with the PBR stabilizing at about 3. The presence of extraneous homoimmune phages appeared to be detrimental to the stability of the pseudolysogens, which were maintained at a lower population density than prophage-free cells in lake water containing the mixed phage (M1) population.

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Author notes

  1. Oladele A. Ogunseitan

    Present address: Program in Social Ecology, University of California, 92717, Irvine, California, USA

Authors and Affiliations

  1. Department of Microbiology and The Graduate Program in Ecology, Center for Environmental Biotechnology, University of Tennessee, 37932-2567, Knoxville, Tennessee

    Oladele A. Ogunseitan & Gary S. Sayler

  2. Department of Biochemistry and Biophysics, The Program in Molecular Biology, Stritch School of Medicine, Loyola University of Chicago, 60153, Maywood, Illinois, USA

    Robert V. Miller

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  1. Oladele A. Ogunseitan
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  2. Gary S. Sayler
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  3. Robert V. Miller
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Ogunseitan, O.A., Sayler, G.S. & Miller, R.V. Dynamic interactions ofPseudomonas aeruginosa and bacteriophages in lake water. Microb Ecol 19, 171–185 (1990). https://doi.org/10.1007/BF02012098

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