Abstract
Pigment extracts of phototrophic bacteria isolated from Lake Kinneret (Rhodopseudomonas palustris, Thiocapsa roseopersicina, Prosthecochloris aestuaris andChlorobium phaeobacteroides) were studied by means of high performance liquid chromatography (HPLC). An absorption wavelength of 360 nm provided the best resolution among the pigments of the species tested and between them and chlorophylla. Signature pigments were identified for each of these species, and their presence was thereby monitored in lake water samples.C. phaeobacteroides, which was observed in the anaerobic hypolimnion and predominated in the metalimnion, was recognized by a characteristic cluster of major chlorophyllous pigment peaks. The spectral qualities of these pigments were close but not identical to published data on bacteriochlorophylle, presumably due to the use of different solvents for extraction. The intensity of these pigment peaks was employed to determine the depth of the greatest phototrophic bacterial biomass, which was not related to that of algae.
Similar content being viewed by others
References
Bergstein T, Henis Y, Cavari BZ (1979) Investigations on the photosynthetic sulfur bacteriumChlorobium phaeobacteroides causing seasonal blooms in Lake Kinneret. Can J Microbiol 25:999–1007
Berman T, Elias Y (1973) Lake Kinneret: Synoptic studies of chlorophyll concentrations. In: Tadmor J (ed) Proc 4th Scientific Conference. Israel Ecol Soc Jerusalem, pp 19–27
Caldwell DE (1977) Accessory pigment fluorescence for quantitation of photosynthetic microbial populations. Can J Microbiol 23:1594–1597
Caraco N, Puccoon AH (1986) The measurement of bacterial chlorophyll and algal chlorophylla in natural waters. Limnol Oceanogr 31:889–893
Eckert W, Frevert T, Bergstein T, Cavari BZ (1986) Competitive development ofThiocapsa roseopersicina andChlorobium phaeobacteroides in Lake Kinneret. Can J Microbiol 32:917–921
Gieskes WW, Kraay GW (1983) Unknown chlorophylla derivatives in the North Sea and the tropical Atlantic Ocean revealed by HPLC analysis. Limnol Oceanogr 28:757–766
Gloe A, Pfennig N, Brockman H Jr, Trowitzsch W (1975) A new bacteriochlorophyll from brown-colored Chlorobiaceae. Arch Microbiol 102:103–109
Klein B, Sournia A (1987) A daily study of the diatom spring bloom at Roscoff (France) in 1985. II. Phytoplankton pigment composition studied by HPLC analysis. Mar Ecol Prog Ser 37:265–275
Korthals HJ, Steenbergen CLM (1985) Separation and quantification of pigments from natural phototrophic microbial populations. FEMS Microbiol Ecol 31:177–185
Mantoura RFC, Llewellyn CA (1983) The rapid determination of algal chlorophyll and carotenoid pigments and their breakdown products in natural waters by reverse phase high-performance liquid chromatography. Anal Chim Acta 151:297–314
Pfennig N (1965) Anreicherungskulturen für rote und grüne Schwefelbakterien. Zentrabl Bakteriol Parasitenkd Infektionskr Hyg Abt 1, Suppl 1, 179–189
Serruya C (1978) Lake Kinneret Monographiae Biologicae. Dr. Junk Publishers, The Hague, 502 p
Stal LJ, van Gemerden H, Krumbein WE (1978) The simultaneous assay of chlorophyll and bacteriochlorophyll in natural microbial communities. J Microbiol Methods 2:295–306
Steenbergen CLM, Korthals HJ (1982) Distribution of phototrophic microorganisms in the anaerobic and microaerophilic strata of Lake Vechten (The Netherlands). Pigment analysis and role in primary production. Limnol Oceanogr 27:883–895
Steenbergen CLM, Korthals HJ (1988) Vertical distribution of pigments in stratified Lake Vechten (The Netherlands), determined by HPLC. Arch Hydrobiol Beih Ergebn Limnol 31:45–53
Wright SW, Shearer JD (1984) Rapid extraction and high-performance liquid chromatography of chlorophylls and carotenoids from marine phytoplankton. J Chromatogr 294:281–295
Zapata M, Ayala AM, Franco JM, Garrido JL (1987) Separation of chlorophylls and their degradation products in marine phytoplankton by reverse-phase high-performance liquid chromatography. Chromatographia 23:26–30
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Yacobi, Y.Z., Eckert, W., Trüper, H.G. et al. High performance liquid chromatography detection of phototrophic bacterial pigments in aquatic environments. Microb Ecol 19, 127–136 (1990). https://doi.org/10.1007/BF02012094
Issue Date:
DOI: https://doi.org/10.1007/BF02012094