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Nitrous oxide production by nitrogen-fixing, fast-growing Rhizobia

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Abstract

Rhizobium trifolii, R. leguminosarum, andR. “hedysarum”, grownex planta under anoxic conditions in a chemically defined medium, evolve N2O from NO3 −, NO2 −, and (NH4)2NO3. The amount of nitrous oxide formed after 96 hours is about 0.2μM×mg−1 cells d.w. Large availability of organic matter enhances the production of N2O from nitrate by free-livingR. trifolii in peat/sand mixtures. Denitrification of the above species andR. meliloti was detected also in planta. Nitrous oxide production increases almost linearly from 10–45μM×mg−1 nodules d.w. when nitrogen-fixing plants are exposed to increasing concentrations of nitrate (1–12μM).

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Authors and Affiliations

  1. Istituto di Microbiologia agraria, Università di Pisa, 56100, Pisa

    S. Casella & C. Leporini

  2. Istituto di Chimica e Industrie agrarie, Università di Padova, 35100, Padova, Italy

    M. P. Nuti

Authors
  1. S. Casella
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  2. C. Leporini
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  3. M. P. Nuti
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Casella, S., Leporini, C. & Nuti, M.P. Nitrous oxide production by nitrogen-fixing, fast-growing Rhizobia. Microb Ecol 10, 107–114 (1984). https://doi.org/10.1007/BF02011418

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