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Growth kinetics ofPseudomonas fluorescens microcolonies within the hydrodynamic boundary layers of surface microenvironments

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Abstract

Computer-enhanced microscopy (CEM) was used to study the growth kinetics of bacterial microcolonies attached to the wall of a continuous-flow slide culture. Image processing increased effective microscope resolution and quantitated colony growth at 10 min intervals. Three growth parameters were used to determine growth rate: the time required for cell fission, the specific rate of increase in cell number, and the specific rate of increase in cell area. Growth rate was initially constant regardless of colony size, as assumed previously in deriving colonization kinetics. However, at low substrate concentrations growth rate varied depending on laminar flow velocity. Growth was flow-dependent at a glucose concentration of 100 mg/liter and flow-independent at a concentration of 1 g/liter. This indicated that the surface microenvironment became substrate-depleted in the absence of sufficient laminar flow velocities and that glucose rather than oxygen was rate limiting.

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References

  1. Berg HC, Block SM (1984) A miniature flow cell designed for rapid exchange of media under high-power microscope objectives. J Gen Microbiol 130:2915–2920

    Google Scholar 

  2. Brannan DK, Caldwell DE (1984) Evaluation of a proposed surface colonization equation usingThermothrix thiopara as a model organism. Microb Ecol 8:14–21

    Google Scholar 

  3. Caldwell DE (1984) Surface colonization parameters from cell density and distribution. In: Marshall KC (ed) Microbial adhesion and aggregation. Springer-Verlag, New York, PP 125–136

    Google Scholar 

  4. Caldwell DE (1985) New developments in computer-enhanced microscopy. J Microbiol Methods 4:117–125

    Google Scholar 

  5. Caldwell DE, Germida JJ (1985) Evaluation of difference imagery for visualizing and quantitating microbial growth. Can J Microbiol 31:35–44

    Google Scholar 

  6. Caldwell DE, Lawrence JR (1986) Study of attached cells in continuous-flow slide culture. In: Wimpenny J (ed) A handbook of laboratory model systems for microbial ecosystem research. CRC Press, Chapter 6. In press

  7. Caldwell DE, Brannan DK, Morris ME, Betlach MR (1981) Quantitation of microbial growth on surfaces. Microb Ecol 7:1–12

    Google Scholar 

  8. Caldwell DE, Kieft TL, Brannan DK (1984) Colonization of sulfide-oxygen interfaces on hot spring tufa byThermothrix thiopara. Geomicrobiol J 3:181–200

    Google Scholar 

  9. Caldwell DE, Malone JA, Kieft TL (1983) Derivation of a growth rate equation describing microbial surface colonization. Microb Ecol 9:1–6

    Google Scholar 

  10. Edwards C (1981) The microbial cell cycle. American Society for Microbiology, Washington, DC

    Google Scholar 

  11. Hirsch P (1984) Microcolony formation and consortia. In: Marshall KC (ed) Microbial adhesion and aggregation. Springer-Verlag, New York, pp 373–394

    Google Scholar 

  12. Kieft TL, Caldwell DE (1983) A computer simulation of surface microcolony formation during microbial colonization. Microb Ecol 9:7–13

    Google Scholar 

  13. Kieft TL, Caldwell DE (1984) Weathering of calcite, pyrite, and sulfur byThermothrix thiopara in a thermal spring. Geomicrobiol J 3:201–216

    Google Scholar 

  14. Kieft TL, Caldwell DE (1984) Chemostat and in situ colonization kinetics ofThermothrix thiopara on calcite and pyrite surfaces. Geomicrobiol J 3:217–229

    Google Scholar 

  15. Kjelleberg S, Hermansson M (1984) Starvation-induced effects on bacterial surface characteristics. Appl Environ Microbiol 48:497–503

    Google Scholar 

  16. Kuhn DA, Starr MP (1970) Effects of microscope illumination on bacterial development. Arch Microbiol 74:282–300

    Google Scholar 

  17. Malone JA, Caldwell DE (1983) Evaluation of two surface colonization equations in continuous culture. Microb Ecol 9:1–6

    Google Scholar 

  18. Marr AG, Painter PR, Nilson EH (1969) Growth and division of individual bacteria. In: Meadow PM, Pirt SJP (eds) Microbial growth. 19th Symposium of the Society for General Microbiology. Cambridge University Press, pp 237–268

  19. Monod J (1950) La technique de culture continui: theorie et applications. Ann Inst Pasteur 79:390–410

    Google Scholar 

  20. Paul JH (1984) Effects of antimetabolites on the adhesion of an estuarineVibrio sp. to polystyrene. Appl Environ Microbiol 48:924–929

    PubMed  Google Scholar 

  21. Perfilev BV, Gabe DR (1969) Capillary methods of investigating microorganisms. Translated by J. M. Shewan. University of Toronto Press

  22. Vogel S (1983) Life in moving fluids: the physical biology of flow. Princeton University Press

  23. Wardell JN, Brown CM, Ellwood DE (1980) A continuous culture study of the attachment of bacteria to surfaces. In: Berkeley RCW, Lynch JM, Melling J, Rutter PR, Vincent B (eds) Microbial adhesion to surfaces. Ellis Horwood Publishers, Chichester, UK, pp 221–230

    Google Scholar 

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Authors and Affiliations

  1. Department of Applied Microbiology and Food Science, Department of Soil Science, University of Saskatchewan, S7N 0W0, Saskatoon, Saskatchewan, Canada

    Douglas E. Caldwell & John R. Lawrence

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  1. Douglas E. Caldwell
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  2. John R. Lawrence
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Caldwell, D.E., Lawrence, J.R. Growth kinetics ofPseudomonas fluorescens microcolonies within the hydrodynamic boundary layers of surface microenvironments. Microb Ecol 12, 299–312 (1986). https://doi.org/10.1007/BF02011173

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  • DOI: https://doi.org/10.1007/BF02011173

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