Abstract
Diel changes in the specific growth rates of natural bacterial communities as a whole and of different groups within the communities were followed over 2 days during July 1982, in stratified waters in the vicinity of a shallow sea tidal mixing front in the Irish Sea. Waters well above (4 m) and below (60 m) the thermocline were enclosed in dialysis bags and incubated in situ. The results show that there were periods of altered growth rates of the whole bacterial community and synchronous cell division of morphological groups. An increase in mean cell volume within both 4 and 60 m communities preceded an increase in specific growth rates, with a resultant decrease in the mean cell volume. Above the thermocline the whole bacterial community, as well as the rod and coccoid forms, doubled in number once a day. The doubling time of the whole bacterial community at 60 m was 2 days and slower than that at 4 m. This was due to a slower doubling time (3 days) for the coccoid forms. Rod forms at the two depths had a similar doubling time (1 day). The time of day when maximum division rate occurred was also different in the two water masses. At 4 m more coccoid forms divided during the night, whereas at 60 m more divided during the day. Conversely, at 4 m more rod forms divided during the day, whereas at 60 m more divided at night.
These data indicate that the bacterial community and members of the community may be adapted to exploit the diurnal rhythms of dissolved organic carbon (DOC) release by other organisms and that portions of the bacterial community may therefore be more active at certain times of the day. The diurnal growth of the bacterial community may thus vary between different water masses and largely reflects the differences in the chemical and biological characteristics of the two water masses investigated.
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References
Burney CM, Johnson KM, Sieburth J McN (1981) Diel flux of dissolved carbohydrate in a salt marsh and a simulated estuarine ecosystem. Mar Biol 63:175–187
Burney CM, Davis PG, Johnson KM, Sieburth J McN (1981) Dependence of dissolved carbohydrate concentrations upon small scale nanoplankton and bacterioplankton distributions in the western Sargasso Sea. Mar Biol 65:289–296
Burney CM, Davis PG, Johnson KM, Sieburth J McN (1982) Diel relationships of microbial trophic groups andin situ dissolved carbohydrate dynamics in the Caribbean Sea. Mar Biol 67:311–322
Carlucci AF, Craven DB, Henricks SM (1984) Diel production and microheterotrophic utilization of dissolved free amino acids in waters off South California. Appl Environ Microbiol 48:165–170
Daley RJ, Hobbie JE (1975) Direct counts of aquatic bacteria by a modified epifluorescence technique. Limnol Oceanogr 20:875–882
Egan B, Floodgate GD (1985) Biological studies in the vicinity of a shallow sea tidal mixing front. II. The distribution of bacteria. Phil Trans R Soc Lond B 310:435–444
Fogg GE, Egan B, Hoy S, Lochte K, Scrope-Howe SOV, Turley CM (1985) Biological studies in the vicinity of a shallow sea tidal mixing front. I. Physical and chemical background. Phil Trans R Soc Lond B 310:407–433
Fogg GE, Floodgate GE, Jones DA, Kassab JY, Lochte K, Rees EIS, Turley CM (1985) Biological studies in the vicinity of a shallow sea tidal mixing front. VII. The frontal ecosystem. Phil Trans R Soc Lond B 310:555–571
Hagström A, Larsson U, Hörstedt P, Normark S (1979) Frequency of dividing cells, a new approach to the determination of bacterial growth rates in aquatic environments. Appl Environ Microbiol 33:805–812
Hobbie JE, Daley RJ, Jasper S (1977) Use of Nuclepore filters for counting bacteria by epifluorescence microscopy. Appl Environ Microbiol 33:1225–1228
Hoppe H-G (1976) Determination and properties of actively metabolizing heterotrophic bacteria in the sea, investigated by means of micro-autoradiography. Mar Biol 36:291–302
Jones JG, Simon BM (1980) An investigation of errors in direct counts of aquatic bacteria by epifluorescence microscopy, with reference to a new method for dyeing filters. J Appl Bacteriol 39:317–329
Krambeck C (1978) Changes in planktonic microbial populations—an analysis by scanning electron microcsopy. Verh Int Verein Limnol 20:2255–2259
Krambeck C (1984) Diurnal responses of microbial activity and biomass in aquatic ecosystems. In: Klug MJ, Reddy CA (eds) Current perspectives in microbial ecology. Proc 3rd Int Symp on Microb Ecol, Michigan State University, 7–12 August 1983. Am Soc Microbiol, Washington DC, pp 502–508
Krambeck C, Krambeck H-J, Overbeck J (1981) Microcomputer-assisted biomass determination of planktonic bacteria on scanning electron micrographs. Appl Environ Microbiol 42:142–149
Larsson U, Hagström A (1982) Fractionated phytoplankton primary production, exudate release and bacterial production in a Baltic eutrophication gradient. Mar Biol 67:57–70
Lochte K (1985) Biological studies in the vicinity of a shallow sea tidal mixing front. III. Seasonal and spatial distribution of heterotrophic uptake of glucose. Phil Trans R Soc Lond B 310:445–469
Lochte K, Turley CM (1985) Heterotrophic activity and carbon flow via bacteria in waters associated with a tidal mixing front. Proc 19th European Marine Biol Symp, Plymouth, U.K., 16–21 September 1984. Cambridge University Press pp 73–85
Meyer-Reil L-A, Bölter M, Liebezeit G, Schramm W (1979) Short-term variations in microbiological and chemical parameters. Mar Ecol Prog Ser 1:1–6
Mopper K, Lindroth P (1982) Diel and depth variations in dissolved free amino acids and ammonium in the Baltic Sea determined by shipboard HPLC analysis. Limnol Oceanogr 27:336–347
Riemann B, Nielson P, Jeppesen M, Marcussen B, Fuhrman JA (1984) Diel changes in bacterial biomass and growth rates in coastal environments, determined by means of thymidine incorporation into DNA, frequency of dividing cells (FDC), and microautoradiography. Mar Ecol Prog Ser 17:227–235
Riemann B, Sondergaard M (1984) Measurements of diel rates of bacterial secondary production in aquatic environments. Appl Environ Microbiol 47:632–638
Scrope-Howe SOV, Jones DA (1985) Biological studies in the vicinity of a shallow sea tidal mixing front. V. The vertical distribution of zooplankton. Phil Trans R Soc Lond B 310:510–519
Sellner KG (1981) Primary productivity and the flux of dissolved organic matter in several marine environments. Mar Biol 65:101–112
Sieburth J McN, Johnson KM, Burney CM, Lavoie DM (1977) Estimation ofin situ rates of heterotrophy using diurnal changes in dissolved organic matter and growth rates of picoplankton in diffusion culture. Helg wiss Meeresunters 30:565–574
Straškrabová V, Fuksa J (1982) Diel changes in numbers and activities of bacterioplankton in a reservoir in relation to algal production. Limnol Oceanogr 27:660–672
Strickland JDH, Parsons TR (1972) A practical handbook of seawater analysis. 2nd ed. Fish Res Bd Can Bull 167:1–310
Turley CM (1985) Biological studies in the vicinity of a shallow sea tidal mixing front. IV. Seasonal and spatial distribution of urea and its uptake by phytoplankton. Phil Trans R Soc Lond B 310:471–500
Turley CM, Lochte K (1985) A direct measurement of bacterial productivity in stratified waters close to a front in the Irish Sea. Mar Ecol Prog Ser 23:209–219
Turley CM, Lochte K (in press) Growth patterns of natural seawater communities incubatedin situ. Proc 2nd Int Colloquium Marine Bacteriol, 1–5 October 1984, Brest, France
van Es FB, Meyer-Reil L-A (1982) Biomass and metabolic activity of heterotrophic bacteria. Adv Microb Ecol 6:111–170
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Turley, C., Lochte, K. Diel changes in the specific growth rate and mean cell volume of natural bacterial communities in two different water masses in the Irish sea. Microb Ecol 12, 271–282 (1986). https://doi.org/10.1007/BF02011170
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DOI: https://doi.org/10.1007/BF02011170