Summary
Vitamin D deficient and phosphate deficient states were produced in young rats by the use of synthetic diets. Whole blood ATP, serum calcium and phosphorus and the ash, phospholipid, hexosamine and hydroxyproline contents of metaphyseal bones of control and rachitic rats were measured after 4 weeks on the diets.
There was a decrease in whole blood ATP and serum phosphorus of the rachitic rats, and in the contents of ash, phospholipid and hexosamine of the rachitic metaphyses, while the hydroxyproline contents of the rachitic bones were higher than those of the controls. Subcutaneous injection of ATP or inorganic phosphate in rats, fed a low phosphate diet deficient in vitamin D, caused an increase in serum phosphorus and blood ATP levels and in metaphyseal ash contents. It is suggested that some of the metabolic disturbances in rickets are due to alteration in ATP production.
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References
Follis, R.H.: Deficiency Disease, p. 557. Ill. Thomas, Springfield, 1958
Harrison, H.E., Harrison, H.C.: The uptake of radiocalcium by the skeleton. The effect of vitamin D and calcium intake. J. Biol. Chem.185, 857–867 (1972)
Meyer, W.L., Kunin, A.S.: The inductive effect of rickets on glycolytic enzymes of rat epiphyseal cartilage, and its reversal by vitamin D phosphate. Arch. Biochem. Biophys.129, 438–446 (1969)
Folch, J., Lees, M., Stanley, G.H.: A simple method for the isolation of, and purification of total lipids from animal tissues. J. Biol. Chem.226, 497–509 (1957)
Marinetti, G.W.: Chromatographic separation, identification and analysis of phosphatides. J. Lipid Res.3, 1–20 (1962)
Stegemann, H.: Mikrobestimmung von hydroxyprolin mit chloramin-T und P-dimethylamino-benzaldehyd. Hoppe-Seyler's Z. Physiol. Chem.311, 41–45 (1958)
Boas, N.F.: Method for the determination of hexosamines in tissues. J. Biol. Chem.204, 553–563 (1953)
Adams, H.: Adenosine 5′-triphosphate, determination with phosphoglyceratekinase. In: Methods of Enzymatic Analysis (Gergmeyer, ed.), pp. 539–543. New York: Academic Press 1973
Bauer, J.D., Ackerman, P.G., Toro, G.: Bray's Clinical Laboratory Methods, p. 331, Seventh Ed. Saint Louis: The C.V. Mosby Co. 1968
Meyer, J.L., McCall, J.T., Smith, L.H.: Inhibition of calcium phosphate crystallization by nucleoside phosphates. Calc. Tiss. Res.15, 287–293 (1974)
Termine, J.D., Conn, K.M.: Inhibition of apatite formation by phosphorylated metabolites and macromolecules. Calcif. Tis. Res.22, 149–157 (1976)
Cartier, P., Picard, J.: La mineralization du cartilage ossifiable. I. La mineralization du cartilage in vitro. Bull. Soc. Chim. Biol.37, 485–494 (1955)
Cruess, R.L., Clark, I.: The effect of vitamin D on the lipids of bone matrix. Proc. Soc. Exp. Biol. Med.136, 415–519 (1971)
Balogh, K., Kunin, A.S.: The effects of vitamin D2 and dietary phosphorus on oxidative enzymes in the epiphyseal cartilage of rachitic rats. Lab. Invest.18, 782–788 (1968)
Travis, S.F., Sugarman, H.F., Ruberg, R.L., Dudrick, S.J., Delivoria-Papadopoulis, M., Miller, L.D., Oski, F.A.: Alterations of red cell glycolytic intermediates and oxygen transport as a consequence of hypophosphatemia in patients receiving intravenous hyperalimentation. N. Engl. J. Med.285, 763–768 (1971)
Hjertquist, S.O.: Autoradiographic study of the epiphyseal cartilage and bone tissue in normal and rachitic rats after administration of radiosulfate. Biochim. Biol. Sperimentale2, 126–140 (1961)
Rapoport, S., Guest, J.M.: Changes of organic acid-soluble phosphorus, diphosphoglycerate, adenosine triphosphate, and inorganic phosphorus in the blood cells of rats during the development and healing of rickets. J. Biol. Chem.,126, 749–761 (1938)
Lehninger, A.: Biochemistry, p. 308. Worth Publishers, New York: 1970
Parsons, V., Self, M.: Urinary hydroxyproline excretion in phosphate depleted rachitic rats. Nature217, 551–552 (1968)
Parsons, V., Hampton, C.: Phosphate deficiency and collagen metabolism. Nutritio et Dieta,13, 46–57 (1969)
Uitto, J., Prokop, D.J.: Hydroxylation of peptide-bond proline and lysine before and after chain completion of the polypeptide chains of procollagen. Arch. Biochem. Biophys.164, 210–217 (1974)
Comstock, J.P., Udenfriend, S.: Effect of lactate on collagen proline hydroxylase activity in cultured L-929 fibroblasts. Proc. Nat. Acad. Sci.66, 552–557 (1970)
Lindy, S., Pedersen, F.B., Turto, H., Uitto, J.: Lactate, lactate dehydrogenase and protocollagen proline hydroxylase in rat skin autograft. Hoppe-Seyler's Z. physiol. Chem. Bd.352, 1113–1118 (1971)
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Hong, K.C., Cruess, R.L. Changes in organic matrix of bone and of bone and blood ATP in rats fed rachitogenic diets. Calc. Tis Res. 25, 241–244 (1978). https://doi.org/10.1007/BF02010776
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DOI: https://doi.org/10.1007/BF02010776